MLH1 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	MLH1
Locus ID	4292
Kit Components	KN201607G1, MLH1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GACAGTGGTGAACCGCATCG KN201607G2, MLH1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCGTGGCAGGGGTTATTCGG KN201607-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GGCGACAGAC CAGGCACAGG GCCCCATCGC CCTCCGGAGG CTCCACCACC AAATAACGCT GGGTCCACTC GGGCCGGAAA ACTAGAGCCT CGTCGACTTC CATCTTGCTT CTTTTGGGCG TCATCCACAT TCTGCGGGAG GCCACAAGAG CAGGGCCAAC GTTAGAAAGG CCGCAAGGGG AGAGGAGGAG CCTGAGAAGC GCCAAGCACC TCCTCCGCTC TGCGCCAGAT CACCTCAGCA GAGGCACACA AGCCCGGTTC CGGCATCTCT GCTCCTATTG GCTGGATATT TCGTATTCCC CGAGCTCCTA AAAACGAACC AATAGGAAGA GCGGACAGCG ATCTCTAACG CGCAAGCGCA TATCCTTCTA GGTAGCGGGC AGTAGCCGCT TCAGGGAGGG ACGAAGAGAC CCAGCAACCC ACAGAGTTGA GAAATTTGAC TGGCATTCAA GCTGTCCAAT CAATAGCTGC CGCTGAAGGG TGGGGCTGGA TGGCGTAAGC TACAGCTGAA GGAAGAACGT GAGCACGAGG CACTGAGGTG ATTGGCTGAA GGCACTTCCG TTGAGCATCT AGACGTTTCC TTGGCTCTTC TGGCGCCAAA GCCAGCTAAT GCTATCAAAG AGATGATTGA GAACTGGTAC GGAGGGAGTC GAGCCGGGCT CACTTAAGGG CTACGACTTA ACGGGCCGCG TCACTCAATG GCGCGGACAC GCCTCTTTGC CCGGGCAGAG GCATGTACAG CGCATGCCCA CAACGGCGGA GGCCGCCGGG TTCCCTGACG TGCCAGTCAG GCCTTCTCCT TTTCCGCAGA CCGTGTGTTT CTTTACCGCT CTCCCCCGAG ACCTTTTAAG GGTTGTTTGG AGTGTAAGTG GAGGAATATA CGTAGTGTTG TCTTAATGGT ACCGTTAACT AAGTAAGGAA GCCACTTAAT TTAAAATTAT GTATGCAGAA CATGCGAAGT TAAAAGATGT ATAAAAGCTT AAGATGGGGA GAAAAACCTT TTTTCAGAGG GTACTGTGTT ACTGTTTTCT TGCTTTTCAT TCATTCCAGA AATCATCTGT TCACATCCAA AGGCACAATT CATTTTGAGT TTCTTTCAAA ACAAATCGTT TGTAGTTTTA GGACAGGCTG ATGCACTTTG GGCTTGACTT CTGATTACCC TATTGTTAAA TTAGTGACCC CTCTTAGTGT TTTCCTGTCC GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_000249, NM_001167617, NM_001167618, NM_001167619, NM_001258271, NM_001258273, NM_001258274, NM_001354615, NM_001354616, NM_001354617, NM_001354618, NM_001354619, NM_001354620, NM_001354621, NM_001354622, NM_001354623, NM_001354624, NM_001354625, NM_001354626, NM_001354627, NM_001354628, NM_001354629, NM_001354630
Synonyms	COCA2; FCC2; hMLH1; HNPCC; HNPCC2
Summary	The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]

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