Mouse Smad4 activation kit by CRISPRa
CAT#: GA202547
Smad4 CRISPRa kit - CRISPR gene activation of mouse SMAD family member 4
CN¥ 12,255.00
Specifications
Product Data | |
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Symbol | Smad4 |
Locus ID | 17128 |
Kit Components | GA202547G1, Smad4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa GA202547G2, Smad4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa GA202547G3, Smad4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa 1 CRISPRa-Enhancer vector, SKU GE100056 1 CRISPRa scramble vector, SKU GE100077 |
Reference Data | |
RefSeq | NM_008540, NM_001364967, NM_001364968 |
Synonyms | AW743858; D18Wsu70e; DPC4; Madh4 |
Summary | Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity). Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (PubMed:15329343). In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8.[UniProtKB/Swiss-Prot Function] |
Documents
Resources
基因表达相关资源 |
Other Versions
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KN316273 | Smad4 - mouse gene knockout kit via CRISPR, HDR mediated |
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KN316273BN | Smad4 - mouse gene knockout kit via CRISPR, HDR mediated |
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KN316273LP | Smad4 - mouse gene knockout kit via CRISPR, HDR mediated |
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KN316273RB | Smad4 - mouse gene knockout kit via CRISPR, HDR mediated |
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KN516273 | Smad4 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. |
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