Mouse Smad4 activation kit by CRISPRa

CAT#: GA202547

Smad4 CRISPRa kit - CRISPR gene activation of mouse SMAD family member 4



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CN¥ 12,255.00


货期*
4周

规格
    • 1 kit

Product images

经常一起买 (2)
Goat Polyclonal Antibody against SMAD4
    • 100 ug

CN¥ 5,371.00


SMAD4 Antibody - middle region
    • 50 ug

CN¥ 4,628.00

Specifications

Product Data
Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
Symbol Smad4
Locus ID 17128
Kit Components

GA202547G1, Smad4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

GA202547G2, Smad4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

GA202547G3, Smad4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

1 CRISPRa-Enhancer vector, SKU GE100056

1 CRISPRa scramble vector, SKU GE100077

Reference Data
RefSeq NM_008540, NM_001364967, NM_001364968
Synonyms AW743858; D18Wsu70e; DPC4; Madh4
Summary Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity). Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (PubMed:15329343). In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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