G (NC_001652) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102272 represents NCBI reference of NP_042679 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACAATGATCACTACTCCTCTGATCTTGATCCTGATCACCTGTGGTGCTAACTCACAGACTGTGA AGCCCGATACCGCCTCAGAGTCCGATCAGCCCACGTGGAGCAACCCGTTGTTCACGTACCCTGAAGGTTG TACTCTCGACAAACTGTCTAAGGTGAACGCAAGTCAGCTTCGCTGTCCCAGAATCTTTGATGACGAGAAC CGCGGTCTGATTGCTTATCCAACGAGCATCCGCTCCTTGAGCGTGGGTAATGACTTGGGGGATATACATA CCCAGGGGAATCACATACACAAAGTGCTGTACCGGACTATTTGCAGCACCGGGTTTTTCGGAGGACAGAC GATCGAAAAGGCCCTCGTGAAGATGAAGCTGTCAACAAAGGAAGCTGGAGCGTACGACACGACCACCGCC GCCGCCCTCTACTTCCCCGCTCCTCGCTGCCAGTGGTACACCGACAACGTCCAGAACGACTTGATCTTTT ACTACACCACCCAAAAATCTGTGCTGCGGGATCCTTATACCAGGGACTTCCTCGATTCTGACTTTATCGG CGGAAAGTGTACAAAATCCCCCTGCCAGACACATTGGTCTAATGTCGTGTGGATGGGAGATGCTGGGATC CCTGCTTGCGATTCATCACAGGAGATCAAAGCCCACCTTTTCGTGGACAAGATTTCTAACAGGGTTGTGA AGGCCACCTCTTACGGCCACCACCCGTGGGGACTGCACCAGGCATGTATGATAGAATTCTGCGGCCAGCA ATGGATTCGGACAGATCTGGGAGATTTGATATCCGTGGTGTATAATTCCGGCAGCGAAATTCTCTCATTC CCTAAGTGTGAAGATAAGACCGTTGGGATGAGGGGCAACCTGGACGATTTCGCCTATCTTGATGATCTGG TGAAGGCAAGTGAAAGTCGAGAAGAATGTCTGGAGGCCCATGCCGAAATCATCTCCACGAATTCAGTGAC TCCTTATCTGCTGTCAAAGTTCAGGTCTCCACATCCAGGAATCAACGATGTGTACGCAATGCACAAGGGT AGTATCTATCACGGCATGTGCATGACCGTTGCAGTCGACGAAGTTTCTAAAGACAGAACCACATACAGGG CACATAGGGCCACCAGCTTTACCAAATGGGAACGACCTTTCGGGGATGAATGGGAGGGGTTTCACGGCTT GCACGGAAATAATACCACGATTATTCCCGATCTGGAGAAATACGTCGCTCAGTACAAGATGTCCATGATG GAGCCCATGAGTATCAAGTCTGTGCCACACCCCTCTATCCTGGCCCTGTATAATGAGACCGATGTCAGCG GGATATCTATCCGGAAGCTGGACTCTTTTGATTTGCAGAGCCTGCACTGGAGTTTTTGGCCCACCATTTC TGCACTCGGTGGCATACCTTTTGTGCTGTTGCTTGCTGTTGCCGCCTGTTGCTGTTGGTCCGGTAGGCCC CCTACTCCTTCAGTCCCTCAGTCTATCCCTATGTACCATCTCGCAAACAGGAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102272 representing NP_042679 Red=Cloning sites Green=Tags MDTMITTPLILILITCGANSQTVKPDTASESDQPTWSNPLFTYPEGCTLDKLSKVNASQLRCPRIFDDEN RGLIAYPTSIRSLSVGNDLGDIHTQGNHIHKVLYRTICSTGFFGGQTIEKALVKMKLSTKEAGAYDTTTA AALYFPAPRCQWYTDNVQNDLIFYYTTQKSVLRDPYTRDFLDSDFIGGKCTKSPCQTHWSNVVWMGDAGI PACDSSQEIKAHLFVDKISNRVVKATSYGHHPWGLHQACMIEFCGQQWIRTDLGDLISVVYNSGSEILSF PKCEDKTVGMRGNLDDFAYLDDLVKASESREECLEAHAEIISTNSVTPYLLSKFRSPHPGINDVYAMHKG SIYHGMCMTVAVDEVSKDRTTYRAHRATSFTKWERPFGDEWEGFHGLHGNNTTIIPDLEKYVAQYKMSMM EPMSIKSVPHPSILALYNETDVSGISIRKLDSFDLQSLHWSFWPTISALGGIPFVLLLAVAACCCWSGRP PTPSVPQSIPMYHLANRS TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_001652
ORF Size	1524 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_001652.1, NP_042679
RefSeq ORF	1524 bp
Locus ID	1489848
MW	56.8 kDa

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