N gene (NC_001652) Virus Tagged ORF Clone
CAT#: VC102269
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid protein [Infectious hematopoietic necrosis virus], codon optimized for human cell expression, NP_042676
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CNY 4,660.00
Cited in 1 publication. |
Product images
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CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC102269 represents NCBI reference of NP_042676 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAAGTGCTCTGAGGGAAACTTTTACTGGGTTGAGAGATATAAAGGGTGGAGTTCTCGAAGACCCTG AGACGGAATACCGCCCCAGCACCATTACTCTCCCACTTTTCTTCTCTAAAACCGACCTGGATCTGGAGAT GATCAAACGCGCCGTGTCTCAGGTCGGCGGGGAAGGCACACGGAAAGCGCTGTCACTGCTGTGCGCCTTC GTCATTGCTGAAACTGTGCCGTCAGAAGCAGGAACCGTGGCCGAGCTGCTGGAGGCTCTTGGCTTCGTGC TGGAATCTTTGGACACAGGAGCACCCCCTGACGCTACATTTGCAGACCCTAACAATAAACTGGCTGAGAC AATAGTTAAAGAGAATGTATTGGAGGTGGTTACCGGACTGCTGTTCACCTGCGCTCTGCTGACAAAATAT GACGTCGATAAGATGGCGACCTATTGTCAGAACAAACTCGAGAGGCTGGCTACAAGCCAGGGTATCGGCG AGCTCGTGAATTTTAACGCGAACAGGGGCGTGCTTGCTAAGATTGGGGCGGTTCTGCGCCCTGGACAGAA ACTGACAAAAGCCATCTACGGGATTATCCTTATCAACCTCAGTGATCCCGCCATAGCTGCGAGAGCTAAG GCCCTGTGCGCGATGAGACTTAGCGGCACCGGAATGACAATGGTGGGCCTCTTCAATCAGGCCGCTAAAA ATCTGGGTGCCCTTCCTGCCGACCTCTTGGAGGACCTTTGCATGAAAAGCGTGGTTGAGAGTGCTCGGCG GATCGTGCGGCTGATGAGGATCGTAGCGGAAGCCCCTGGAGTGGCCGCCAAATACGGCGTCATGATGTCA CGAATGCTGGGTGAGGGATATTTTAAGGCATACGGCATAAACGAGAACGCACGGATTACCTGTATCCTCA TGAACATCAACGATCGGTACGACGATGGCACAAGCAGGGGGTTGACTGGGATCAAGGTGAGTGATCCCTT TAGGAAGCTGGCAAGAGAGATCGCCAGGCTGCTCGTGTTGAAATACGATGGCGACGGCTCTACAGGGGAA GGAGCTTCCGAGCTCATCCGAAGAGCAGAGATGGCATCACGGGGTCCAGACATGGGTGAGGAAGAGGAAG AGGATGAGGAAGACGATGACTCATCTGAGCCAGGAGATTCCGATAGCTTTCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102269 representing NP_042676
Red=Cloning sites Green=Tags MTSALRETFTGLRDIKGGVLEDPETEYRPSTITLPLFFSKTDLDLEMIKRAVSQVGGEGTRKALSLLCAF VIAETVPSEAGTVAELLEALGFVLESLDTGAPPDATFADPNNKLAETIVKENVLEVVTGLLFTCALLTKY DVDKMATYCQNKLERLATSQGIGELVNFNANRGVLAKIGAVLRPGQKLTKAIYGIILINLSDPAIAARAK ALCAMRLSGTGMTMVGLFNQAAKNLGALPADLLEDLCMKSVVESARRIVRLMRIVAEAPGVAAKYGVMMS RMLGEGYFKAYGINENARITCILMNINDRYDDGTSRGLTGIKVSDPFRKLAREIARLLVLKYDGDGSTGE GASELIRRAEMASRGPDMGEEEEEDEEDDDSSEPGDSDSFH TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001652 |
ORF Size | 1173 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NC_001652.1, NP_042676 |
RefSeq ORF | 1173 bp |
Locus ID | 1489845 |
MW | 42.2 kDa |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Overexpression of the nucleocapsid protein of Middle East Respiratory Syndrome coronavirus up-regulates CXCL10
,Aboagye, JO;Yew, CW;Ng, OW;Monteil, VM;Mirazimi, A;Tan, YJ;,
Biosci. Rep.
,PubMed ID 30242057
[N gene]
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VC102271 | Myc-DDK-tagged ORF clone of viral ORF for matrix protein [Infectious hematopoietic necrosis virus], codon optimized for human cell expression, NP_042678 |
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VC102272 | Myc-DDK-tagged ORF clone of viral ORF for glycoprotein [Infectious hematopoietic necrosis virus], codon optimized for human cell expression, NP_042679 |
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VC102273 | Myc-DDK-tagged ORF clone of viral ORF for non-virion protein [Infectious hematopoietic necrosis virus], codon optimized for human cell expression, NP_042680 |
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