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L1 (NC_014955) Virus Tagged ORF Clone
CAT＃: VC101883
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for L1 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169290
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CNY 6,180.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101883 represents NCBI reference of YP_004169290 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCTCTCTGGTTGCCATCTGCCGGACCTCTGTACCTCCCCCCTGCCAAACCCACCGCGCGGGTGC TTCGCACTGACGAATATGTAACCGGCACAGACGTCTATTTTCATGCCGGCTCTGATCGCCTTCTGATAGT TGGACACCCGTATTTTAACGTCTACACCGGCAACGATAACAATAAGAAGCTTAGTATCCCTAAAGTGTCT AGTTGTCAGTACCGGGTCTTCCACTGCAAACTCCCTGACCCTAATAGGTTCGCCCTCATAGACCAGAAAC TCTACAATCCTGACAGAGAGCGACTCGTTTGGAAGTTCACCGGCATGGAGATTGGAAGGGGTGGCCCTTT GGGTATTTCCAGCACTGGCCATCCATTGTATAATAAAATTCGCGACACGGAGAATCCTAATCAGTACCCA GGGGACGGGGCAGAGCAGCGCATGGATTTCTCCATGGATCCCAAGCAGGTCCAACTCTTCATCGTGGGGT GCGCCCCTGCCACAGGGGAATACTGGGACGTGGCAAAGAAGTGCGAAGAAGGCCAGAAGGGTGACTGTCC CGCTATTCAGCTTGTGAACAGCACAATACAAGACGGTGACATGGGCGACATCGGATTCGGTGCAGCCAAT TTTAAAGCCCTGCAGGAAGATAGGTCTTCTGTGTCCTTGGATCTCGTGGATACATTTTCAATCTGGCCAG ATTACCTGCGGATGACCAAGGACATCTACGGGGATTCCCTCTTCTTCCACGGCAGGCGCGAGCAACAGTT CGCTCGCCACTTGTGGGCTCGGGCAGGCACAATGGGTGACGCAATTCCTGAGAGCAACTATCTTCACCCG GCAGAAGGCTCTCCGCAAGAAACCAATCTCGGATCTCACATTTATTTTCCCACAGCATCCGGCTCCCTCG CTTCTTCTGACAACAATCTTTTTAATCGGCCCTACTGGCTTCGGAGAGCCCAGGGGACCAACAATGGAAT ATGCTGGGGCAATAGCCTCTTTCTGACGGTGTTGGACAATACAAGAGGAACAAATTTCACTATCTCAGTA CTCAAAGAGGATGGTAAAACACTCGACCAGAATTACAAATACAACGCGGCCGACTTTAAGCAGTACACCC GACACACCGAAGAGTACGAGTTGGAGTTTATTTTTCAGTTGTGTAAGGTCAAATTGGAGCCTGACGTACT TGCTCACATCAATGTGATGAACCCTAAAATTCTGGATCAGTGGCAGCTCGCATTTGTACCCCCCCCTCAG ACTGCCCTGGAGGACGCATATCGATATCTCAGATCCAATGCCACCCGATGCCACCTCCCTGATGAGAACG AGGAGAGCCCTGATCCATACGCCAAGATGAATTTTTGGACGGTGGATCTGCAAGAGAGATTCTCAAGCGA TCTTAGTCAGCACGCCCTGGGTCGGAAGTTTCTTTACCAGATAGGACTGCTGAATGGGAAACGCCCTCGG ACTGTGTATGCAGAGCAAAGCGGTACTAAGACTGTGAAAAGGAAACGCACAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101883 representing YP_004169290
Red=Cloning sites Green=Tags MSSLWLPSAGPLYLPPAKPTARVLRTDEYVTGTDVYFHAGSDRLLIVGHPYFNVYTGNDNNKKLSIPKVS SCQYRVFHCKLPDPNRFALIDQKLYNPDRERLVWKFTGMEIGRGGPLGISSTGHPLYNKIRDTENPNQYP GDGAEQRMDFSMDPKQVQLFIVGCAPATGEYWDVAKKCEEGQKGDCPAIQLVNSTIQDGDMGDIGFGAAN FKALQEDRSSVSLDLVDTFSIWPDYLRMTKDIYGDSLFFHGRREQQFARHLWARAGTMGDAIPESNYLHP AEGSPQETNLGSHIYFPTASGSLASSDNNLFNRPYWLRRAQGTNNGICWGNSLFLTVLDNTRGTNFTISV LKEDGKTLDQNYKYNAADFKQYTRHTEEYELEFIFQLCKVKLEPDVLAHINVMNPKILDQWQLAFVPPPQ TALEDAYRYLRSNATRCHLPDENEESPDPYAKMNFWTVDLQERFSSDLSQHALGRKFLYQIGLLNGKRPR TVYAEQSGTKTVKRKRTK TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_014955 |
| ORF Size | 1524 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_014955.1, YP_004169290 |
| RefSeq ORF | 1524 bp |
| Locus ID | 10146749 |
| MW | 57.7 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101878 | Myc-DDK-tagged ORF clone of viral ORF for E1 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169286 |
CNY 7,220.00 |
|
| VC101879 | Myc-DDK-tagged ORF clone of viral ORF for E2 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169287 |
CNY 4,660.00 |
|
| VC101880 | Myc-DDK-tagged ORF clone of viral ORF for E4, partial [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169288 |
CNY 3,800.00 |
|
| VC101881 | Myc-DDK-tagged ORF clone of viral ORF for E6 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169284 |
CNY 3,800.00 |
|
| VC101882 | Myc-DDK-tagged ORF clone of viral ORF for E7 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169285 |
CNY 3,800.00 |
|
| VC101884 | Myc-DDK-tagged ORF clone of viral ORF for L2 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169289 |
CNY 6,180.00 |
