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E2 (NC_014955) Virus Tagged ORF Clone
CAT＃: VC101879
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for E2 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169287
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CNY 4,660.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101879 represents NCBI reference of YP_004169287 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGATGGAGACACAAGAGACTCTGACGGCCCGCTTTGTCGCTCAGCAGGAAATCCAGCTGGCCCTGA TCGAAAAGGAAAGCACAAACCTGCAGGATCATATCCAGTACTGGTCAGCTGTTCGGAAAGAACACGTCCT TGCCTACTACGCGCGACAGGAGGGGTATACTCGGCTTGGGCTGCAGCCTCTGGCAAGCACAGCCGTCTCC GAATACCGGGCCAAAGAAGCCATTCACATTGTGATTCTCCTCAAATCCCTGCTGAAATCCGAGTATGGCC CAGAACTCTGGACTCTCGCTGAAGTAAGTGCCGAAATTTTGAATACTCCTCCTAAGAACTGTTTCAAAAA AGGTCCATTCACCGTCACCGTCTACTTCGACAATGACACAAACAACAGCTTCCCCTATACATGTTGGAAC TACATTTATTATCAGGATGATAACAACCGGTGGCACAAGACCGAAGGGCAAGTTGACGCCAACGGTCTCT ATTTTAAGGAAAAGAACGGCGACGTCACTTATTTTACCGTCTTCCAGCCAGACGCCGAAAGATACGGACA TACTGGCCAGTGGACCATCAAGTATAAGTCTCAGACCATCTCCGCATCTGTTTCTTCATTTAGCCGGTCT GTCCCTGAACCTCCTCCGGAGAGCCCGGAACCATCCCCCGATACCCCATCAACATCAAAAGTTTCCGGTA AGCGCAGAAGAGAGACAGAGGAAGATTCACACCCGGAGAGCCCAAGTTCAAGCAATATAGAACTCAGGAG AAGAAGGGGCAACCAGCAGAGAAAAAGCACCACCAGACTGAGAGGAGGGGCAGCGGGTCGCTCCAGCAAC AGCGCACCAACACCTGAAGAGGTTGGTGCAGGAACTAGATCCGTCCCCAAGCACGGACTTAGCGGACTGC GGAGGCTCCAGGAGGAAGCTCGCGACCCGCCTCTCATCCTTCTGAAAGGGCATCCTAATACCTTGAAATG TTGGAGGTGGCGGTGTAAAAACAAGTACTCACACCTGTTTCTCGCGTGCACTACCGTTTTCCACTGGGTC GGCGATGACACAAATAAATCTACCAGCGCCCGGGTGCTGCTGGCGTTCGCCACGGTCTGCGAGCGCCAGA CATTTCTTAATACAGTCCCCCTGCCCAAAGGAACCTCCTATGTACTGGGGAATCTGGATTCACTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101879 representing YP_004169287
Red=Cloning sites Green=Tags MQMETQETLTARFVAQQEIQLALIEKESTNLQDHIQYWSAVRKEHVLAYYARQEGYTRLGLQPLASTAVS EYRAKEAIHIVILLKSLLKSEYGPELWTLAEVSAEILNTPPKNCFKKGPFTVTVYFDNDTNNSFPYTCWN YIYYQDDNNRWHKTEGQVDANGLYFKEKNGDVTYFTVFQPDAERYGHTGQWTIKYKSQTISASVSSFSRS VPEPPPESPEPSPDTPSTSKVSGKRRRETEEDSHPESPSSSNIELRRRRGNQQRKSTTRLRGGAAGRSSN SAPTPEEVGAGTRSVPKHGLSGLRRLQEEARDPPLILLKGHPNTLKCWRWRCKNKYSHLFLACTTVFHWV GDDTNKSTSARVLLAFATVCERQTFLNTVPLPKGTSYVLGNLDSL TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_014955 |
| ORF Size | 1185 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_014955.1, YP_004169287 |
| RefSeq ORF | 1185 bp |
| Locus ID | 10146746 |
| MW | 44.8 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101878 | Myc-DDK-tagged ORF clone of viral ORF for E1 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169286 |
CNY 7,220.00 |
|
| VC101880 | Myc-DDK-tagged ORF clone of viral ORF for E4, partial [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169288 |
CNY 3,800.00 |
|
| VC101881 | Myc-DDK-tagged ORF clone of viral ORF for E6 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169284 |
CNY 3,800.00 |
|
| VC101882 | Myc-DDK-tagged ORF clone of viral ORF for E7 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169285 |
CNY 3,800.00 |
|
| VC101883 | Myc-DDK-tagged ORF clone of viral ORF for L1 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169290 |
CNY 6,180.00 |
|
| VC101884 | Myc-DDK-tagged ORF clone of viral ORF for L2 [Human papillomavirus type 132], codon optimized for human cell expression, YP_004169289 |
CNY 6,180.00 |
