L2 (NC_014185) Virus Tagged ORF Clone

CAT#: VC101801

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for minor capsid protein L2 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668030



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CNY 6,370.00


货期*
4周

规格
    • 10 ug

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101801 represents NCBI reference of YP_003668030 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATACTAGTAAAAGAGTTAAGCGCGATTCTGTCAAGCACCTGTACGAGCAGTGTAAGATTACAGGTA
ATTGCCCGGACGACGTCAAAAATAAAGTCGAAGGAACCACCTTGGCGGACAGGCTGCTGCAATGGCTCGG
ATCAGTTATCTACCTTGGAGGTCTTGGAATCGGAACTGGCAAGGGATCTGGCGGATCAACCGGCTATACA
CCTCTCGGCCGGACACCCGGAGTTGGGTCCAGTGGGACCGTGGTACGCCCCACTATTCCCCTGGATCCGC
TGGGGCCTACCGATATCATACCCATTGATACGGTGGATCCTGCAGGCCCTGCACTGGTGCCCCTCAATGA
AGGCAACTTCCCAGATGTGACGGTGGTCGATGCTGGGGGAGCTACGGAGGACGTGACTGTAAGCGGCCCC
GACCTCGGGTCAGGGGAAATCGCAATTATAACGGAGTCAGACCCAATTAGTGATGTCACAACCACTGGGG
GGCACCCCACGGTTTCAACCACCGAGGACAATGTTGCTGTTATTGACGTCCAGACCGAGCCACCTCCTCC
TAAGCGGCTCGCCTTCGAGAGAGGACCGCGGACATCCACCCCAACGCACATATCTGTTACACAGTCTATA
GCAAACATAAGCGATCCCGGGAACATATTTGTAGATGCGCACTTCACTGGCCAGACAATCGGAGAAGAGA
TTCCATTGACTAACCTGGGTTCTTTCGCTGAATTCGAGATCGAAGAGGCAGCTTCTGCTCCCAAACATTC
AACCCCGAAAGCTTTCCAACGAGCCTATAGTAAGGCACGCGATTTGTACAACCGGAGGGTTCAGCAGGTT
CAGACAAGAAATGTTCATTTTTTGAATCAGCCATCACGGCTGGTCCAATTCGAGTTTACAAACCCCGCCT
TTGAGGAGGAGCAAATCTCCCTTCAGTTTCAACAGGACGTGCAACAGCTCGCAGCCGCACCCGACCCTGA
CTTCCAAGACATTATCACACTCGGACGACCCCGGTTTTCCGAGACTGCGGATAATAGGATTCGGGTTAGT
CGCCTGGGTCGACGGGGCACCATTCGCACCCGATCTGGCCTGCAGATTGGGCAGCAGGTGCACTTCTATC
TCGATCTGTCCACTATTGACAATTCCGAGGCAATCGAATTGGGTGTGCTTGGGGAACATACCGGCGATGC
TAGTATCATTAACCCCCAGGCCGAGTCCTCTTTTATTGATCTTCTGAGTGAGGAGCCAATCGCCCACACG
GAGGATGAACTGTTGGACGATGTAGTGGAAGATTTCGGGAACGCGCATCTGATTCTGACGAGCAACACTC
GCACACAGAGCATTACTATGCCGACCATTCCTCCGGGAGTCGCCCTGAAGGTATTCGTAACAGACATCGC
CCAGCCCCTTTTTGTCTCATATCCACAAACCAACATTATTCCGGGCATACTGCCGACGACACCGACCTCC
AACATCGAACCTGCAATCATTATCGACCTGGAGTCCGCTTCCGACTTCTTCATCCACCCAAGTCTCCTGA
AGCGAAAAAGGAAGAGACCACTGCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101801 representing YP_003668030
Red=Cloning sites Green=Tags

MNTSKRVKRDSVKHLYEQCKITGNCPDDVKNKVEGTTLADRLLQWLGSVIYLGGLGIGTGKGSGGSTGYT
PLGRTPGVGSSGTVVRPTIPLDPLGPTDIIPIDTVDPAGPALVPLNEGNFPDVTVVDAGGATEDVTVSGP
DLGSGEIAIITESDPISDVTTTGGHPTVSTTEDNVAVIDVQTEPPPPKRLAFERGPRTSTPTHISVTQSI
ANISDPGNIFVDAHFTGQTIGEEIPLTNLGSFAEFEIEEAASAPKHSTPKAFQRAYSKARDLYNRRVQQV
QTRNVHFLNQPSRLVQFEFTNPAFEEEQISLQFQQDVQQLAAAPDPDFQDIITLGRPRFSETADNRIRVS
RLGRRGTIRTRSGLQIGQQVHFYLDLSTIDNSEAIELGVLGEHTGDASIINPQAESSFIDLLSEEPIAHT
EDELLDDVVEDFGNAHLILTSNTRTQSITMPTIPPGVALKVFVTDIAQPLFVSYPQTNIIPGILPTTPTS
NIEPAIIIDLESASDFFIHPSLLKRKRKRPLL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_014185
ORF Size 1566 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_014185.1, YP_003668030
RefSeq ORF 1566 bp
Locus ID 9231103
MW 56.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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