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E2 (NC_014185) Virus Tagged ORF Clone
CAT＃: VC101799
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for regulatory protein E2 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668028
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CNY 4,660.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101799 represents NCBI reference of YP_003668028 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGCTGCGCGAACGCTTCGATGCTGTGCAAGAGGCGCTCCTGACACTTTATGAAAACGCCGCCA AAGACATTGATACCCAGATCCAGCATTGGGAGCTGCTCAGACAGGAGCACGTCATCATGTACTATGCTAG ACAGAACGGAATAAAGCGCATCGGATTCCACCAGGTGCCAAGCTTGGCAACATCCGAACACAAAGCCAAG GAGGCCATCCGGATGACAATCCTGCTCAAATCTTTGAAAGACTCTCCTTTCGGGTCTGAGACATGGAGTC TTACGGAAACATCCCTGGAAATTGTCAACGCCGCACCGAGTAACTGCTTTAAGAAGGAAGCATATATCGT GGATGTGTATTATGATCGCGATGCGGATAAAGTTTATCCATACACAGGGTGGAAGCATATTTATTTTCAG GGCGAAGACAATATGTGGCACAAAACCCCTGGCCGCGTGGACTACTATGGTCTGTACTACATCGATGACT ATGGTGACAAAGTCTATTATGTGAACTTCGACGAGAAAGCCCAGACGCTTTCCGTCACAGGAGAGTGGGA GGTAAAATACCAAAATAAGACTATCTCTCCCTCCGTTACTTCTAGCTCTAGCCCCACCGCCTGGAAACCC GCTCAGGGCCACACTTCTCACTCCCCCTGGAATAGTGAAACAATAGAGGAGGTCAGAAGAAAACAAGAAG GCCCAAAGAACACCTCTGTTCCCTCCTCCGAAGAGACACCCGGACTGCGGCATAACAGAAGAAGACGAGA GGGTGAGAGCGGAATCACCAGGTCTCCCAAGCGGCGGCGGACCGATATTCCAGACAGCACCGGCCCTGCG TACCCAACTCCAGCCCAGGTAGGTAGTAGCCATCGGTCCGCGTCCGGGCACTATAGTTCCCGGCTTGAAC GACTGCAGGCGGAAGCCAGAGACCCGCCCATAATTATTGTTAAGGGATGCGCTAACACACTTAAATGCTG GAGGTTCCGGTTCAAGAAACAGAATAGAAAACTGTTCGAGCAGGCGTCAACCGTGTTTAAATGGGTGGAT TATGACAGTTCCAGCCGGATGCTGCTTTCATTCAAGGATACAGCACAGAGACAGCTGTTTCTCGACACCG TTACTTTGCCTAAAGGAACCCAGGTTGCTCTGGGGAGTCTTAACAGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101799 representing YP_003668028
Red=Cloning sites Green=Tags MEKLRERFDAVQEALLTLYENAAKDIDTQIQHWELLRQEHVIMYYARQNGIKRIGFHQVPSLATSEHKAK EAIRMTILLKSLKDSPFGSETWSLTETSLEIVNAAPSNCFKKEAYIVDVYYDRDADKVYPYTGWKHIYFQ GEDNMWHKTPGRVDYYGLYYIDDYGDKVYYVNFDEKAQTLSVTGEWEVKYQNKTISPSVTSSSSPTAWKP AQGHTSHSPWNSETIEEVRRKQEGPKNTSVPSSEETPGLRHNRRRREGESGITRSPKRRRTDIPDSTGPA YPTPAQVGSSHRSASGHYSSRLERLQAEARDPPIIIVKGCANTLKCWRFRFKKQNRKLFEQASTVFKWVD YDSSSRMLLSFKDTAQRQLFLDTVTLPKGTQVALGSLNSL TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_014185 |
| ORF Size | 1170 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_014185.1, YP_003668028 |
| RefSeq ORF | 1170 bp |
| Locus ID | 9231101 |
| MW | 44.9 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101796 | Myc-DDK-tagged ORF clone of viral ORF for transforming protein E6 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668025 |
CNY 3,800.00 |
|
| VC101797 | Myc-DDK-tagged ORF clone of viral ORF for transforming protein E7 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668026 |
CNY 3,800.00 |
|
| VC101798 | Myc-DDK-tagged ORF clone of viral ORF for replication protein E1 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668027 |
CNY 7,320.00 |
|
| VC101800 | Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein, partial [Human papillomavirus 121], codon optimized for human cell expression, YP_003668029 |
CNY 3,800.00 |
|
| VC101801 | Myc-DDK-tagged ORF clone of viral ORF for minor capsid protein L2 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668030 |
CNY 6,370.00 |
|
| VC101802 | Myc-DDK-tagged ORF clone of viral ORF for major capsid protein L1 [Human papillomavirus 121], codon optimized for human cell expression, YP_003668031 |
CNY 6,270.00 |
