helicase-primase subunit BBLF2/3 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101055

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BBLF2/BBLF3 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129478



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CNY 10,640.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101055 represents NCBI reference of YP_001129478 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGAGACACCAGCGGAGTCAGTGAGAGCACGGGTCTCTAGTGTTACCTTCTATAACGTGACACAAA
CCGCAGGCCGGTGGTGGGCCATTTGGGTTGTGGGGATCGTGCCGATTAAACGAGAGGACGTTGAGACTCT
GATTGTTGTGCAGGCATGCCAGCCACCACTGGGAGGGAGTCTGGAACCCCCAGTGGTCAACGCGCCCAGC
ACAACCGAGCTGAATTTTCTGCGGTGGGAGAGGGAACTGCGCAGGTCCGGAGGACTGATCGCCATGCTGG
CTGACGCTGCTGAGAAAGACCTCTTTGATCTCTCCTTCAGAACTCGGGATCGGCGATTGCTCAGCGCCGC
TAGAGTTGAGGATGAGCAGGGCCTGATTTTCCAGCCACTGTTCCCCGCCCAGGTCGTGTGCCAGAGCTGC
AGTGGGGATGATGGCCGCGACCAACAGCCACCTCCAGTGGACGGATTCGGCAGCGAAATGGAGGGAGAGC
AGACTTGTCCCCACGCACAGAGACACTCTGAATCACCTGGCCAGCTTGATGTGTATATCCGGACCCCACG
CGGAGACGTTTTTACATATAGTACCGAAACCCCAGATGATCCCTCCCCTGTACCCTTTCGGGATATCCTG
AGGCCTGTTACATATGAAGTGGACCTGGTGAGCTCTGACGGTGCAACAGGGCGGGGGGGAGACGCTCGAC
GCCACAGAGTCTCCCTTAAGATCCTCGAGCCCGCCGGAGGCTTTGAGAGCTGGCTTGTCAACTCATGGAG
CATGGCAGGTGGAGGCTTGTATGCCTTTCTTAGATCCATCTATGCCTCATGCTACGCCAACCACCGCGGA
ACAAAACCGATTTTCTATCTCCTTGATCCTGAACTCTGCCCCGGTGGCAGCGACTTCCAGCCATACGTCC
CGGGATTCCCCTTTCTTCCTATACACTACGTGGGCCGCGCTCGCCCTGCCTTTTGGCATCGCGCACCTCA
CTCAGAGGGTCTGCTGCTGCTGGATCTGAATCTGGGCGTGAGCGGTACTCCCCTTGCCGACGCACTGCTG
GGGCTGGACGCGCGCAGCGGACAGCGCCGCGGATCTCTCCTCCTGCAGCAAATCTGGCCTCCTACCCGAA
AGGAAATAAACCCCAGGCATGTTTGCACCCGGGAAGGCGGTGAAGGTGGTGGCGAAGACGAGACGACCGT
AGTGGGCAGGGCCGAGGCCACGGCAATTTTGGAAGCAGATGCAACATGGTGGCTGTATGAACTGGCACGG
TGTCATCTGAGCGCGAGAGGAGCCCCCGTGGGGACACCCGATGGTGGGGGACAGGCCCGCGACGCTCAGA
CATGGCTCAGAGCCCTCCACAGATATGGGACATCCGACACTCGGAGAGCGCTCGGGGGATTGTATACCGC
AGTGACTCGGGTACTGTTGCACGCGGCCGCGGACCTCGGCCTGACATGGGCATATGCCGATGAGTTTATC
CTGGGATTTGTGGCTCCAACCTCTGCTCACCCATCTGAGGAGCCCCTCGCTCAGGCCTTCCTGCAGGGGG
TGAAGGATTCTGAGGACGCAAGCCGGCTGGATAGGGACGTAATGGGAGGCGAAGCTACCGTCGCAAGGCG
CCACATCCGAGTCAAAGCTCGAAGAGGCCCTGGGTGTCTCTTGATGGCCATCTTCCAAGGAGATCTGTAC
GTGGGCGGGTGTCGCGAGCACTCCGGTCCCTTCCTGGTATGGCACGAGGCATTTTCTTGGACGCTGGATC
AGCTGGCCGCGCGGCCCGAGGCAGATAAGGCCCCTCCATCTCATGACCATCTGCTGACGCTTGTTAGAGA
CCTGACTAGACGCCTGGCGCCAGGACGGAGGCGCAATCGATTTTGGGCGCTGCCTCGGGCTTGGCTGCAG
CGGCTGCGGAGAGCAGGACTGAGGCTGAGTGGCTCACATGTCTGCCTTCTGGACAAAGATGGAGCCCGCC
CCGCACCATGCCAGACTGCGACTGAGCACGGCTTGTCCCCTACCGCATATTTCCGGGAGATCATGGCCTT
TCTGCTGGATGTAATCAGCGCTCTGCACCCTGGCTATACAATTCCTATGGAGATTACTAGAGAGACAGAC
CTGCTCATGACAGTGCTGTCACTGTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101055 representing YP_001129478
Red=Cloning sites Green=Tags

MMETPAESVRARVSSVTFYNVTQTAGRWWAIWVVGIVPIKREDVETLIVVQACQPPLGGSLEPPVVNAPS
TTELNFLRWERELRRSGGLIAMLADAAEKDLFDLSFRTRDRRLLSAARVEDEQGLIFQPLFPAQVVCQSC
SGDDGRDQQPPPVDGFGSEMEGEQTCPHAQRHSESPGQLDVYIRTPRGDVFTYSTETPDDPSPVPFRDIL
RPVTYEVDLVSSDGATGRGGDARRHRVSLKILEPAGGFESWLVNSWSMAGGGLYAFLRSIYASCYANHRG
TKPIFYLLDPELCPGGSDFQPYVPGFPFLPIHYVGRARPAFWHRAPHSEGLLLLDLNLGVSGTPLADALL
GLDARSGQRRGSLLLQQIWPPTRKEINPRHVCTREGGEGGGEDETTVVGRAEATAILEADATWWLYELAR
CHLSARGAPVGTPDGGGQARDAQTWLRALHRYGTSDTRRALGGLYTAVTRVLLHAAADLGLTWAYADEFI
LGFVAPTSAHPSEEPLAQAFLQGVKDSEDASRLDRDVMGGEATVARRHIRVKARRGPGCLLMAIFQGDLY
VGGCREHSGPFLVWHEAFSWTLDQLAARPEADKAPPSHDHLLTLVRDLTRRLAPGRRRNRFWALPRAWLQ
RLRRAGLRLSGSHVCLLDKDGARPAPCQTATEHGLSPTAYFREIMAFLLDVISALHPGYTIPMEITRETD
LLMTVLSLF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_009334
ORF Size 2127 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_009334.1, YP_001129478
RefSeq ORF 2127 bp
Locus ID 5176202
MW 78.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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