BMRF2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101032

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BMRF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129455



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CNY 4,280.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

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Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101032 represents NCBI reference of YP_001129455 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTTCTTGCAAACAACATCTGTCCTTGGGGGCCTGCGTGTTCTGCTTGGGATTGTTGGCCTCAACTC
CCTTTATCTGGTGTTTCGTGTTTGCCAACCTCCTCAGCCTGGAAATTTTCAGTCCCTGGCAGACCCACGT
CTATCGCTTGGGCTTTCCTACAGCCTGCCTGATGGCCGTACTCTGGACCCTGGTCCCTGCTAAGCACGCC
GTGAGGGCAGTTACGCCAGCCATAATGCTTAACATCGCGTCAGCCCTTATCTTCTTCTCCCTGAGGGTCT
ACTCAACATCCACATGGGTTAGTGCACCCTGTCTGTTTCTGGCCAATCTCCCCCTGTTGTGCTTGTGGCC
CCGCCTGGCAATCGAGATAGTTTACATTTGTCCTGCTATACACCAGCGCTTCTTCGAACTGGGTCTGCTG
CTTGCTTGTACAATTTTTGCCTTGTCAGTGGTGAGCCGAGCACTGGAGGTCAGCGCAGTATTTATGAGCC
CCTTTTTTATTTTTCTGGCCCTTGGGTCCGGCTCACTGGCCGGGGCACGCCGGAATCAGATCTACACTAG
TGGATTGGAGCGACGCAGGTCCATCTTTTGTGCTCGAGGGGACCATAGCGTGGCATCCCTGAAAGAGACT
CTTCACAAATGCCCCTGGGATCTCCTTGCTATTTCCGCCCTCACAGTGCTCGTGGTGTGCGTGATGATCG
TGCTGCATGTCCACGCAGAAGTGTTCTTCGGCCTCTCACGGTATCTCCCGCTGTTCCTCTGTGGCGCCAT
GGCAAGCGGGGGACTGTATCTCGGTCACTCCTCCATCATCGCCTGCGTTATGGCCACTCTGTGCACCTTG
TCCAGCGTGGTTGTGTATTTTCTTCATGAAACGTTGGGTCCTCTGGGAAAGACCGTGCTTTTTATTTCAA
TCTTCGTGTATTACTTCAGTGGCGTGGCCGCTCTCAGCGCCGCCATGAGGTACAAGCTCAAGAAATTCGT
AAATGGACCTCTCGTGCATCTTAGAGTTGTATATATGTGTTGTTTCGTCTTCACCTTTTGCGAATACCTG
CTGGTTACTTTTATTAAAAGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101032 representing YP_001129455
Red=Cloning sites Green=Tags

MFSCKQHLSLGACVFCLGLLASTPFIWCFVFANLLSLEIFSPWQTHVYRLGFPTACLMAVLWTLVPAKHA
VRAVTPAIMLNIASALIFFSLRVYSTSTWVSAPCLFLANLPLLCLWPRLAIEIVYICPAIHQRFFELGLL
LACTIFALSVVSRALEVSAVFMSPFFIFLALGSGSLAGARRNQIYTSGLERRRSIFCARGDHSVASLKET
LHKCPWDLLAISALTVLVVCVMIVLHVHAEVFFGLSRYLPLFLCGAMASGGLYLGHSSIIACVMATLCTL
SSVVVYFLHETLGPLGKTVLFISIFVYYFSGVAALSAAMRYKLKKFVNGPLVHLRVVYMCCFVFTFCEYL
LVTFIKS

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_009334
ORF Size 1071 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_009334.1, YP_001129455
RefSeq ORF 1071 bp
Locus ID 5176179
MW 39.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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