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2A protein (NC_001428) Virus Tagged ORF Clone
CAT＃: VC100744
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for 2A protein [Human enterovirus C], codon optimized for human cell expression, NP_740587
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CNY 3,800.00
| Cited in 1 publication. |
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC100744 represents NCBI reference of NP_740587 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTTGGTCACCAAAATAAAGCCGTATATGTCGCTGGGTATAAAATCTGCAATTACCACCTTGCTA CACCCTCTGACCACCTCAATGCTATATCCGTGCTCTGGGATCGCGATCTGATGGTCGTTGAGTCAAGGGC ACAAGGCACTGATACCATAGCCAGGTGCAGCTGCAGGTGCGGAGTCTACTATTGCGAGTCACGGAGGAAA TACTATCTGGTGACTTTCACCGGACCAACATTTAGGTTTATGGAGGCCAACGATTATTACCCCGCACGCT ACCAATCCCATATGTTGATTGGGTGTGGGTTCGCGGAACCTGGTGATTGCGGGGGGATTCTGAGGTGCAC ACACGGTGTGATCGGCATTATCACAGCTGGGGGCGAAGGTATCGTGGCCTTTGCTGATATCAGGGATTTG TGGGTATACGAAGAGGAGGCGATGGAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100744 representing NP_740587
Red=Cloning sites Green=Tags MGFGHQNKAVYVAGYKICNYHLATPSDHLNAISVLWDRDLMVVESRAQGTDTIARCSCRCGVYYCESRRK YYLVTFTGPTFRFMEANDYYPARYQSHMLIGCGFAEPGDCGGILRCTHGVIGIITAGGEGIVAFADIRDL WVYEEEAMEQ TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001428 |
| ORF Size | 450 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001428.1, NP_740587 |
| RefSeq ORF | 450 bp |
| MW | 16.8 kDa |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Lysosome-associated membrane proteins-1 and -2 (LAMP-1 and LAMP-2) assemble via distinct modes.
,null,
Biochemical and biophysical research communications
,PubMed ID 27663661
[2A protein]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC100740 | Myc-DDK-tagged ORF clone of viral ORF for VP4 protein [Human enterovirus C], codon optimized for human cell expression, NP_740583 |
CNY 3,800.00 |
|
| VC100741 | Myc-DDK-tagged ORF clone of viral ORF for VP2 protein [Human enterovirus C], codon optimized for human cell expression, NP_740584 |
CNY 3,800.00 |
|
| VC100742 | Myc-DDK-tagged ORF clone of viral ORF for VP3 protein [Human enterovirus C], codon optimized for human cell expression, NP_740585 |
CNY 3,800.00 |
|
| VC100743 | Myc-DDK-tagged ORF clone of viral ORF for VP1 protein [Human enterovirus C], codon optimized for human cell expression, NP_740586 |
CNY 3,800.00 |
|
| VC100745 | Myc-DDK-tagged ORF clone of viral ORF for 2B protein [Human enterovirus C], codon optimized for human cell expression, NP_740588 |
CNY 3,800.00 |
|
| VC100746 | Myc-DDK-tagged ORF clone of viral ORF for 2C protein [Human enterovirus C], codon optimized for human cell expression, NP_740589 |
CNY 3,800.00 |
|
| VC100747 | Myc-DDK-tagged ORF clone of viral ORF for 3A protein [Human enterovirus C], codon optimized for human cell expression, NP_740590 |
CNY 3,800.00 |
|
| VC100748 | Myc-DDK-tagged ORF clone of viral ORF for VPg (3B) protein [Human enterovirus C], codon optimized for human cell expression, NP_740591 |
CNY 3,800.00 |
|
| VC100749 | Myc-DDK-tagged ORF clone of viral ORF for protease (3C) protein [Human enterovirus C], codon optimized for human cell expression, NP_740592 |
CNY 3,600.00 |
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