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protease (3C) protein (NC_001428) Virus Tagged ORF Clone
CAT＃: VC100749
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for protease (3C) protein [Human enterovirus C], codon optimized for human cell expression, NP_740592
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CNY 3,600.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC100749 represents NCBI reference of NP_740592 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACCTGGGTTCGACTACGCCGTGGCCATGGCGAAGAGAAACATTGTTACTGCCACTACCACCAAGG GAGAGTTCACCATGCTCGGCGTCCACGACAATGTGGCCATCCTCCCTACCCACGCTGCTCCAGGCGAGAC TATTATTGTAGACGGCAAGGAGGTCGAAATCCTGGATGCTCGGGCTCTGGAGGACCAAGCCGGGACAAAT CTGGAGATAACGATTATCACATTGAAACGGAACGAGAAATTCCGCGATATTAGACCGCACATCCCAACAC AGATCACAGAGACCAACGATGGCGTGCTCATCGTGAATACCAGTAAGTATCCCAATATGTATGTGCCCGT GGGGGCCGTCACAGAACAAGGATACCTGAATCTGAGTGGGCGACAGACGGCGCGCACCTTGATGTATAAC TTTCCTACCCGCGCTGGGCAATGCGGCGGAATCATCACTTGCACCGGGAAGGTCATCGGCATGCATGTGG GTGGAAATGGTTCTCACGGTTTCGCCGCAGCCCTGAAGAGGAGCTACTTCACCCAGAACCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100749 representing NP_740592
Red=Cloning sites Green=Tags MGPGFDYAVAMAKRNIVTATTTKGEFTMLGVHDNVAILPTHAAPGETIIVDGKEVEILDARALEDQAGTN LEITIITLKRNEKFRDIRPHIPTQITETNDGVLIVNTSKYPNMYVPVGAVTEQGYLNLSGRQTARTLMYN FPTRAGQCGGIITCTGKVIGMHVGGNGSHGFAAALKRSYFTQNQ TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001428 |
| ORF Size | 552 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001428.1, NP_740592 |
| RefSeq ORF | 552 bp |
| MW | 19.9 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC100740 | Myc-DDK-tagged ORF clone of viral ORF for VP4 protein [Human enterovirus C], codon optimized for human cell expression, NP_740583 |
CNY 3,800.00 |
|
| VC100741 | Myc-DDK-tagged ORF clone of viral ORF for VP2 protein [Human enterovirus C], codon optimized for human cell expression, NP_740584 |
CNY 3,800.00 |
|
| VC100742 | Myc-DDK-tagged ORF clone of viral ORF for VP3 protein [Human enterovirus C], codon optimized for human cell expression, NP_740585 |
CNY 3,800.00 |
|
| VC100743 | Myc-DDK-tagged ORF clone of viral ORF for VP1 protein [Human enterovirus C], codon optimized for human cell expression, NP_740586 |
CNY 3,800.00 |
|
| VC100744 | Myc-DDK-tagged ORF clone of viral ORF for 2A protein [Human enterovirus C], codon optimized for human cell expression, NP_740587 |
CNY 3,800.00 |
|
| VC100745 | Myc-DDK-tagged ORF clone of viral ORF for 2B protein [Human enterovirus C], codon optimized for human cell expression, NP_740588 |
CNY 3,800.00 |
|
| VC100746 | Myc-DDK-tagged ORF clone of viral ORF for 2C protein [Human enterovirus C], codon optimized for human cell expression, NP_740589 |
CNY 3,800.00 |
|
| VC100747 | Myc-DDK-tagged ORF clone of viral ORF for 3A protein [Human enterovirus C], codon optimized for human cell expression, NP_740590 |
CNY 3,800.00 |
|
| VC100748 | Myc-DDK-tagged ORF clone of viral ORF for VPg (3B) protein [Human enterovirus C], codon optimized for human cell expression, NP_740591 |
CNY 3,800.00 |
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