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E3 (NC_001460) Virus Tagged ORF Clone
CAT＃: VC100285
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640221
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CNY 3,800.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC100285 represents NCBI reference of YP_002640221 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAGTATCTTTCTCCTCTTCCTGTTCAGTCTCCCCAGTGGCCTGTATGCCCAGACTGCCGAGCGCC CCTTGAAGGTTGTTGTAGAGGCCGGGCACAATGTAACACTCCCCCACCTGTCAGGGAGTCACCAGACCGG CCACGTGACCTGGCTGGTCGAGACCAGCGACTATGGATCTGCGAGTCCTGATAATTTTATCTTTTCCGGA CAGAAGCTGTGTCAGTTTACAGATAGAACAATGGTTTGGCCGTATTACAATCTGCACTTTAACTGCGAGA ACTACGATCTGAACCTTTTCTGGCTGAAAGTAGAGAACTCCGCCATCTATAATGTGAAAAATACAGTGAA TGCCTCAGAAACGAACATCTATTATGACCTGAGGGTCGTCCAAATATTCCCTCCCAAGTGCATCATCACC TCTAAGTACCTGACGAACGATTACTGTCATATTACCATCAACTGCACCAACTCTGATTACCCCAATAAGG TAGTCTTCAATAACGTCTCACGGTGGTATTATGGGTACGGCAAAGGCAGCCCTACCCTGCCAAATTACTT CATCACAAACTTCAATGTGAGTGGCATTACAAAGAGCTTTAATCACACCTACCCGTTCAATGAACTCTGC GACTATCCTACCTCCCAATCACAACACTCTCTCACACACACGGTTTCTACTGTTATTTTTCTTGGAATTA TTGGCTTCTCCATTCTGATCATCATCGCGGCCTTTATCTATTTGTGTTGGCACCGAAAAAGCCTGTGCGT CAGCAAAACTGAGCCACTCATGCCAATCCCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100285 representing YP_002640221
Red=Cloning sites Green=Tags MLSIFLLFLFSLPSGLYAQTAERPLKVVVEAGHNVTLPHLSGSHQTGHVTWLVETSDYGSASPDNFIFSG QKLCQFTDRTMVWPYYNLHFNCENYDLNLFWLKVENSAIYNVKNTVNASETNIYYDLRVVQIFPPKCIIT SKYLTNDYCHITINCTNSDYPNKVVFNNVSRWYYGYGKGSPTLPNYFITNFNVSGITKSFNHTYPFNELC DYPTSQSQHSLTHTVSTVIFLGIIGFSILIIIAAFIYLCWHRKSLCVSKTEPLMPIPY TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001460 |
| ORF Size | 804 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001460.1, YP_002640221 |
| RefSeq ORF | 804 bp |
| Locus ID | 1460862 |
| MW | 30.7 kDa |
Documents
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Resources
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| VC100261 | Myc-DDK-tagged ORF clone of viral ORF for E2B gene product [Human adenovirus A], codon optimized for human cell expression, NP_040917 |
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| VC100262 | Myc-DDK-tagged ORF clone of viral ORF for L1 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040918 |
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| VC100263 | Myc-DDK-tagged ORF clone of viral ORF for L1 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040919 |
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| VC100264 | Myc-DDK-tagged ORF clone of viral ORF for L2 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040920 |
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| VC100265 | Myc-DDK-tagged ORF clone of viral ORF for L2 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040921 |
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| VC100266 | Myc-DDK-tagged ORF clone of viral ORF for L2 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040922 |
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| VC100267 | Myc-DDK-tagged ORF clone of viral ORF for L3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040923 |
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| VC100268 | Myc-DDK-tagged ORF clone of viral ORF for L3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040924 |
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| VC100269 | Myc-DDK-tagged ORF clone of viral ORF for L3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040925 |
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| VC100270 | Myc-DDK-tagged ORF clone of viral ORF for E2A-L gene product [Human adenovirus A], codon optimized for human cell expression, NP_040926 |
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| VC100271 | Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040927 |
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| VC100272 | Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040928 |
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| VC100273 | Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040929 |
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| VC100274 | Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040930 |
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| VC100275 | Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040932 |
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| VC100276 | Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040931 |
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| VC100277 | Myc-DDK-tagged ORF clone of viral ORF for L5 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040933 |
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| VC100278 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040934 |
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| VC100279 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040935 |
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| VC100280 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040936 |
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| VC100281 | Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_597783 |
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| VC100282 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, NP_597784 |
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| VC100283 | Myc-DDK-tagged ORF clone of viral ORF for L4 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640219 |
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| VC100284 | Myc-DDK-tagged ORF clone of viral ORF for E3 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640220 |
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| VC100286 | Myc-DDK-tagged ORF clone of viral ORF for U gene product, partial [Human adenovirus A], codon optimized for human cell expression, YP_002640222 |
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| VC100287 | Myc-DDK-tagged ORF clone of viral ORF for L2 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640218 |
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| VC100288 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640223 |
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| VC100289 | Myc-DDK-tagged ORF clone of viral ORF for E4 gene product [Human adenovirus A], codon optimized for human cell expression, YP_002640224 |
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