L2 (NC_001460) Virus Tagged ORF Clone

CAT#: VC100264

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for L2 gene product [Human adenovirus A], codon optimized for human cell expression, NP_040920



  View other clones from "Virus" (35)

Need custom modification / cloning service?
Get a free quote

CNY 6,080.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100264 represents NCBI reference of NP_040920 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAGAGCAGTCGAACTTCAGACAGTGGCCTTCCCCGAAACACCCCCTCCTAGTTACGAAACAGTAA
TGGCTGCGGCCCCCCCTTATGTGCCGCCAAGATACCTCGGACCAACAGAGGGGAGGAATAGCATCCGATA
TTCTGAACTGTCACCTTTGTACGATACCACACGGGTCTACCTGGTGGATAACAAATCCAGCGATATCGCT
TCACTGAACTACCAGAACGATCACAGCAACTTTCTCACAACTGTGGTGCAGAATAATGATTACTCTCCGA
TCGAAGCTGGGACACAGACCATCAACTTCGATGAACGGAGCAGATGGGGCGGGGATTTGAAAACAATTCT
GCACACGAACATGCCTAATGTCAACGACTTCATGTTTACAACAAAGTTCAAGGCCAGGGTAATGGTCGCC
AGAAAAACTAACAATGAAGGGCAAACTATCCTCGAGTACGAATGGGCCGAATTTGTCTTGCCCGAGGGGA
ATTACAGTGAAACAATGACCATTGATCTTATGAATAATGCCATTATTGAACACTATCTCCGCGTGGGCAG
ACAGCATGGCGTTTTGGAAAGCGATATCGGTGTAAAGTTTGATACAAGAAACTTCAGACTGGGGTGGGAC
CCAGAGACCCAGCTCGTAACCCCTGGGGTATACACCAACGAAGCGTTTCATCCCGACATCGTATTGCTTC
CCGGTTGTGGGGTTGATTTTACAGAGTCCAGACTGTCTAATATACTCGGCATTAGGAAAAGGCAGCCCTT
TCAAGAGGGCTTCGTCATTATGTATGAACATCTCGAGGGCGGTAACATCCCCGCACTCCTCGACGTCAAG
AAGTACGAGAACAGCCTTCAGGACCAGAACACAGTACGAGGGGATAACTTCATCGCTTTGAACAAGGCTG
CAAGGATTGAACCAGTAGAGACAGACCCCAAGGGGAGGAGCTACAACCTTCTGCCAGACAAGAAGAATAC
GAAGTACAGGTCATGGTATCTGGCGTACAATTATGGGGATCCGGAAAAGGGAGTGAGGTCCTGGACTTTG
TTGACGACCCCTGATGTCACAGGGGGTAGTGAGCAGGTCTACTGGAGTCTGCCCGATATGATGCAGGACC
CAGTGACATTTCGCTCCTCCCGGCAGGTAAGTAACTACCCTGTTGTCGCCGCGGAGCTGCTGCCAGTTCA
CGCCAAATCCTTCTACAATGAGCAGGCTGTCTACTCACAGCTGATACGCCAGTCTACCGCCCTCACAAGA
GTGTTCAACCGCTTCCCAGAGAATCAAATCCTGGTCAGACCTCCGGCCGCTACAATTACTACAGTAAGTG
AGAACGTTCCAGCGCTGACAGATCACGGTACGCTTCCCCTTAGGTCTTCTATCTCTGGAGTTCAGAGGGT
GACAATCACGGACGCCCGGAGACGCACCTGTCCATACGTCTACAAGGCCCTCGGTATTGTTTCTCCCAGG
GTGCTGTCCAGTAGGACTTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100264 representing NP_040920
Red=Cloning sites Green=Tags

MRRAVELQTVAFPETPPPSYETVMAAAPPYVPPRYLGPTEGRNSIRYSELSPLYDTTRVYLVDNKSSDIA
SLNYQNDHSNFLTTVVQNNDYSPIEAGTQTINFDERSRWGGDLKTILHTNMPNVNDFMFTTKFKARVMVA
RKTNNEGQTILEYEWAEFVLPEGNYSETMTIDLMNNAIIEHYLRVGRQHGVLESDIGVKFDTRNFRLGWD
PETQLVTPGVYTNEAFHPDIVLLPGCGVDFTESRLSNILGIRKRQPFQEGFVIMYEHLEGGNIPALLDVK
KYENSLQDQNTVRGDNFIALNKAARIEPVETDPKGRSYNLLPDKKNTKYRSWYLAYNYGDPEKGVRSWTL
LTTPDVTGGSEQVYWSLPDMMQDPVTFRSSRQVSNYPVVAAELLPVHAKSFYNEQAVYSQLIRQSTALTR
VFNRFPENQILVRPPAATITTVSENVPALTDHGTLPLRSSISGVQRVTITDARRRTCPYVYKALGIVSPR
VLSSRTF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001460
ORF Size 1491 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001460.1, NP_040920
RefSeq ORF 1491 bp
Locus ID 1460868
MW 56.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...