MICAL1 (NM_001286613) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MICAL; MICAL-1; NICAL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286613, the custom clone sequence may differ by one or more nucleotides ATGTCTTGTCTAAGCCACTCATCTCTGCCCAGCTGCTGCCCTCCCCAGGAGGCCTCCATGGCTTCACCTA CCTCCACCAACCCAGCGCATGCCCACTTTGAGAGCTTCCTGCAGGCCCAGCTGTGCCAGGACGTGCTGAG CAGCTTCCAGGAGCTGTGTGGGGCCCTGGGGCTGGAACCCGGTGGGGGGCTGCCCCAGTACCACAAGATC AAGGACCAGCTCAACTACTGGAGCGCCAAGTCACTGTGGACCAAGCTGGACAAGCGAGCAGGCCAGCCTG TCTACCAGCAGGGCCGGGCCTGCACCAGCACCAAGTGCCTGGTGGTGGGTGCTGGACCTTGCGGGCTGCG GGTCGCTGTGGAGCTGGCGCTGCTGGGGGCCCGAGTGGTGCTGGTGGAAAAGCGCACCAAGTTCTCTCGC CACAACGTGCTCCACCTCTGGCCCTTCACCATCCACGACCTGCGGGCACTCGGTGCTAAGAAGTTCTACG GGCGCTTCTGCACCGGCACCCTGGACCACATCAGCATCAGGCAGCTCCAGCTGCTTCTGCTGAAGGTAGC ATTGCTGCTGGGGGTGGAAATTCACTGGGGTGTCACTTTCACTGGCCTCCAGCCCCCTCCTAGGAAGGGG AGTGGCTGGCGTGCCCAGCTCCAACCCAACCCCCCTGCCCAGCTGGCCAACTATGAATTTGACGTCCTTA TCTCGGCTGCAGGAGGTAAATTCGTCCCTGAAGGCTTCAAAGTTCGAGAAATGCGAGGCAAACTGGCCAT TGGCATCACAGCCAACTTTGTGAATGGACGCACCGTGGAGGAGACACAGGTGCCGGAGATCAGTGGTGTA GCCAGGATCTACAACCAGAGCTTCTTCCAGAGCCTTCTCAAAGCCACAGGCATTGATCTGGAGAACATTG TGTACTACAAGGACGACACCCACTACTTTGTGATGACAGCCAAGAAGCAGTGCCTGCTGCGGCTGGGGGT GCTGCGCCAGGACTGGCCAGACACCAATCGGCTGCTGGGCAGTGCCAATGTGGTGCCCGAGGCTCTGCAG CGCTTTACCCGGGCAGCTGCTGACTTTGCCACCCATGGCAAGCTCGGGAAACTAGAGTTTGCCCAGGATG CCCATGGGCAGCCTGATGTCTCTGCCTTTGACTTCACGAGCATGATGCGGGCAGAGAGTTCTGCTCGTGT GCAAGAGAAGCATGGCGCCCGCCTGCTGCTGGGACTGGTGGGGGACTGCCTGGTGGAGCCCTTCTGGCCC CTGGGCACTGGAGTGGCACGGGGCTTCCTGGCAGCCTTTGATGCAGCCTGGATGGTGAAGCGGTGGGCAG AGGGCGCTGAGTCCCTAGAGGTGTTGGCTGAGCGTGAGAGCCTGTACCAGCTTCTGTCACAGACATCCCC AGAAAACATGCATCGCAATGTGGCCCAGTATGGGCTGGACCCAGCCACCCGCTACCCCAACCTGAACCTC CGGGCAGTGACCCCCAATCAGGTACGAGACCTGTATGATGTGCTAGCCAAGGAGCCTGTGCAGAGGAACA ACGACAAGACAGATACAGGGATGCCAGCCACCGGGTCGGCAGGCACCCAGGAGGAGCTGCTACGCTGGTG CCAGGAGCAGACAGCTGGGTACCCGGGAGTCCACGTCTCCGATTTGTCTTCCTCCTGGGCTGATGGGCTA GCTCTGTGTGCCCTGGTGTACCGGCTGCAGCCTGGCCTGCTGGAACCCTCAGAGCTGCAGGGGCTGGGAG CTCTGGAAGCAACTGCTTGGGCACTAAAGGTGGCAGAGAATGAGCTGGGCATCACACCGGTGGTGTCTGC ACAGGCCGTGGTAGCAGGGAGTGACCCACTGGGCCTCATTGCCTACCTCAGCCACTTCCACAGTGCCTTC AAGAGCATGGCCCACAGCCCAGGCCCTGTCAGCCAGGCCTCCCCAGGGACCTCCAGTGCTGTATTATTCC TTAGTAAACTTCAGAGGACCCTGCAGCGATCCCGGGCCAAGGAAAATGCAGAGGATGCTGGTGGCAAGAA GCTGCGCTTGGAGATGGAGGCCGAGACCCCAAGTACTGAGGTGCCACCTGACCCAGAGCCTGGTGTACCC CTGACACCCCCATCCCAACACCAGGAGGCCGGTGCTGGGGACCTGTGTGCACTTTGTGGGGAACACCTCT ATGTCCTGGAACGCCTCTGTGTCAACGGCCATTTCTTCCACCGGAGCTGCTTCCGCTGCCATACCTGTGA GGCCACACTGTGGCCAGGTGGCTACGAGCAGCACCCAGGAGATGGACATTTCTACTGCCTCCAGCACCTG CCCCAGACAGACCACAAAGCGGAAGGCAGCGATAGAGGCCCTGAGAGTCCGGAGCTCCCCACACCAAGTG AGAATAGCATGCCACCAGGCCTCTCAACTCCCACAGCCTCGCAGGAGGGGGCCGGTCCTGTTCCAGATCC CAGCCAGCCCACCCGTCGGCAGATCCGCCTCTCCAGCCCGGAGCGCCAGCGGTTGTCCTCCCTTAACCTT ACCCCTGACCCGGAAATGGAGCCTCCACCCAAGCCTCCCCGCAGCTGCTCCGCCTTGGCCCGCCACGCCC TGGAGAGCAGCTTTGTGGGCTGGGGCCTGCCAGTCCAGAGCCCTCAAGCTCTTGTGGCCATGGAGAAGGA GGAAAAAGAGAGTCCCTTCTCCAGTGAAGAGGAAGAAGAAGATGTGCCTTTGGACTCAGATGTGGAACAG GCCCTGCAGACCTTTGCCAAGACCTCAGGCACCATGAATAACTACCCAACATGGCGTCGGACTCTGCTGC GCCGTGCGAAGGAGGAGGAGATGAAGAGGTTCTGCAAGGCCCAGACCATCCAACGGCGACTAAATGAGAT TGAGGCTGCCTTGAGGGAGCTAGAGGCCGAGGGCGTGAAGCTGGAGCTGGCCTTGAGGCGCCAGAGCAGT TCCCCAGAACAGCAAAAGAAACTATGGGTAGGACAGCTGCTACAGCTCGTTGACAAGAAAAACAGCCTGG TGGCTGAGGAGGCCGAGCTCATGATCACGGTGCAGGAATTGAATCTGGAGGAGAAACAGTGGCAGCTGGA CCAGGAGCTACGAGGCTACATGAACCGGGAAGAAAACCTAAAGACAGCTGCTGATCGGCAGGCTGAGGAC CAGGTCCTGAGGAAGCTGGTGGATTTGGTCAACCAGAGAGATGCCCTCATCCGCTTCCAGGAGGAGCGCA GGCTCAGCGAGCTGGCCTTGGGGACAGGGGCCCAGGGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001286613
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286613.1, NP_001273542.1
RefSeq Size	3697 bp
RefSeq ORF	3261 bp
Locus ID	64780
UniProt ID	Q8TDZ2
Gene Summary	This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (3) represents the longest transrcript and encodes the longest isoform (3).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.