MICAL1 (NM_001286613) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MICAL; MICAL-1; NICAL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239951 representing NM_001286613 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTGTCTAAGCCACTCATCTCTGCCCAGCTGCTGCCCTCCCCAGGAGGCCTCCATGGCTTCACCTA CCTCCACCAACCCAGCGCATGCCCACTTTGAGAGCTTCCTGCAGGCCCAGCTGTGCCAGGACGTGCTGAG CAGCTTCCAGGAGCTGTGTGGGGCCCTGGGGCTGGAACCCGGTGGGGGGCTGCCCCAGTACCACAAGATC AAGGACCAGCTCAACTACTGGAGCGCCAAGTCACTGTGGACCAAGCTGGACAAGCGAGCAGGCCAGCCTG TCTACCAGCAGGGCCGGGCCTGCACCAGCACCAAGTGCCTGGTGGTGGGTGCTGGACCTTGCGGGCTGCG GGTCGCTGTGGAGCTGGCGCTGCTGGGGGCCCGAGTGGTGCTGGTGGAAAAGCGCACCAAGTTCTCTCGC CACAACGTGCTCCACCTCTGGCCCTTCACCATCCACGACCTGCGGGCACTCGGTGCTAAGAAGTTCTACG GGCGCTTCTGCACCGGCACCCTGGACCACATCAGCATCAGGCAGCTCCAGCTGCTTCTGCTGAAGGTAGC ATTGCTGCTGGGGGTGGAAATTCACTGGGGTGTCACTTTCACTGGCCTCCAGCCCCCTCCTAGGAAGGGG AGTGGCTGGCGTGCCCAGCTCCAACCCAACCCCCCTGCCCAGCTGGCCAACTATGAATTTGACGTCCTTA TCTCGGCTGCAGGAGGTAAATTCGTCCCTGAAGGCTTCAAAGTTCGAGAAATGCGAGGCAAACTGGCCAT TGGCATCACAGCCAACTTTGTGAATGGACGCACCGTGGAGGAGACACAGGTGCCGGAGATCAGTGGTGTA GCCAGGATCTACAACCAGAGCTTCTTCCAGAGCCTTCTCAAAGCCACAGGCATTGATCTGGAGAACATTG TGTACTACAAGGACGACACCCACTACTTTGTGATGACAGCCAAGAAGCAGTGCCTGCTGCGGCTGGGGGT GCTGCGCCAGGACTGGCCAGACACCAATCGGCTGCTGGGCAGTGCCAATGTGGTGCCCGAGGCTCTGCAG CGCTTTACCCGGGCAGCTGCTGACTTTGCCACCCATGGCAAGCTCGGGAAACTAGAGTTTGCCCAGGATG CCCATGGGCAGCCTGATGTCTCTGCCTTTGACTTCACGAGCATGATGCGGGCAGAGAGTTCTGCTCGTGT GCAAGAGAAGCATGGCGCCCGCCTGCTGCTGGGACTGGTGGGGGACTGCCTGGTGGAGCCCTTCTGGCCC CTGGGCACTGGAGTGGCACGGGGCTTCCTGGCAGCCTTTGATGCAGCCTGGATGGTGAAGCGGTGGGCAG AGGGCGCTGAGTCCCTAGAGGTGTTGGCTGAGCGTGAGAGCCTGTACCAGCTTCTGTCACAGACATCCCC AGAAAACATGCATCGCAATGTGGCCCAGTATGGGCTGGACCCAGCCACCCGCTACCCCAACCTGAACCTC CGGGCAGTGACCCCCAATCAGGTACGAGACCTGTATGATGTGCTAGCCAAGGAGCCTGTGCAGAGGAACA ACGACAAGACAGATACAGGGATGCCAGCCACCGGGTCGGCAGGCACCCAGGAGGAGCTGCTACGCTGGTG CCAGGAGCAGACAGCTGGGTACCCGGGAGTCCACGTCTCCGATTTGTCTTCCTCCTGGGCTGATGGGCTA GCTCTGTGTGCCCTGGTGTACCGGCTGCAGCCTGGCCTGCTGGAACCCTCAGAGCTGCAGGGGCTGGGAG CTCTGGAAGCAACTGCTTGGGCACTAAAGGTGGCAGAGAATGAGCTGGGCATCACACCGGTGGTGTCTGC ACAGGCCGTGGTAGCAGGGAGTGACCCACTGGGCCTCATTGCCTACCTCAGCCACTTCCACAGTGCCTTC AAGAGCATGGCCCACAGCCCAGGCCCTGTCAGCCAGGCCTCCCCAGGGACCTCCAGTGCTGTATTATTCC TTAGTAAACTTCAGAGGACCCTGCAGCGATCCCGGGCCAAGGAAAATGCAGAGGATGCTGGTGGCAAGAA GCTGCGCTTGGAGATGGAGGCCGAGACCCCAAGTACTGAGGTGCCACCTGACCCAGAGCCTGGTGTACCC CTGACACCCCCATCCCAACACCAGGAGGCCGGTGCTGGGGACCTGTGTGCACTTTGTGGGGAACACCTCT ATGTCCTGGAACGCCTCTGTGTCAACGGCCATTTCTTCCACCGGAGCTGCTTCCGCTGCCATACCTGTGA GGCCACACTGTGGCCAGGTGGCTACGAGCAGCACCCAGGAGATGGACATTTCTACTGCCTCCAGCACCTG CCCCAGACAGACCACAAAGCGGAAGGCAGCGATAGAGGCCCTGAGAGTCCGGAGCTCCCCACACCAAGTG AGAATAGCATGCCACCAGGCCTCTCAACTCCCACAGCCTCGCAGGAGGGGGCCGGTCCTGTTCCAGATCC CAGCCAGCCCACCCGTCGGCAGATCCGCCTCTCCAGCCCGGAGCGCCAGCGGTTGTCCTCCCTTAACCTT ACCCCTGACCCGGAAATGGAGCCTCCACCCAAGCCTCCCCGCAGCTGCTCCGCCTTGGCCCGCCACGCCC TGGAGAGCAGCTTTGTGGGCTGGGGCCTGCCAGTCCAGAGCCCTCAAGCTCTTGTGGCCATGGAGAAGGA GGAAAAAGAGAGTCCCTTCTCCAGTGAAGAGGAAGAAGAAGATGTGCCTTTGGACTCAGATGTGGAACAG GCCCTGCAGACCTTTGCCAAGACCTCAGGCACCATGAATAACTACCCAACATGGCGTCGGACTCTGCTGC GCCGTGCGAAGGAGGAGGAGATGAAGAGGTTCTGCAAGGCCCAGACCATCCAACGGCGACTAAATGAGAT TGAGGCTGCCTTGAGGGAGCTAGAGGCCGAGGGCGTGAAGCTGGAGCTGGCCTTGAGGCGCCAGAGCAGT TCCCCAGAACAGCAAAAGAAACTATGGGTAGGACAGCTGCTACAGCTCGTTGACAAGAAAAACAGCCTGG TGGCTGAGGAGGCCGAGCTCATGATCACGGTGCAGGAATTGAATCTGGAGGAGAAACAGTGGCAGCTGGA CCAGGAGCTACGAGGCTACATGAACCGGGAAGAAAACCTAAAGACAGCTGCTGATCGGCAGGCTGAGGAC CAGGTCCTGAGGAAGCTGGTGGATTTGGTCAACCAGAGAGATGCCCTCATCCGCTTCCAGGAGGAGCGCA GGCTCAGCGAGCTGGCCTTGGGGACAGGGGCCCAGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239951 representing NM_001286613 Red=Cloning site Green=Tags(s) MSCLSHSSLPSCCPPQEASMASPTSTNPAHAHFESFLQAQLCQDVLSSFQELCGALGLEPGGGLPQYHKI KDQLNYWSAKSLWTKLDKRAGQPVYQQGRACTSTKCLVVGAGPCGLRVAVELALLGARVVLVEKRTKFSR HNVLHLWPFTIHDLRALGAKKFYGRFCTGTLDHISIRQLQLLLLKVALLLGVEIHWGVTFTGLQPPPRKG SGWRAQLQPNPPAQLANYEFDVLISAAGGKFVPEGFKVREMRGKLAIGITANFVNGRTVEETQVPEISGV ARIYNQSFFQSLLKATGIDLENIVYYKDDTHYFVMTAKKQCLLRLGVLRQDWPDTNRLLGSANVVPEALQ RFTRAAADFATHGKLGKLEFAQDAHGQPDVSAFDFTSMMRAESSARVQEKHGARLLLGLVGDCLVEPFWP LGTGVARGFLAAFDAAWMVKRWAEGAESLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNL RAVTPNQVRDLYDVLAKEPVQRNNDKTDTGMPATGSAGTQEELLRWCQEQTAGYPGVHVSDLSSSWADGL ALCALVYRLQPGLLEPSELQGLGALEATAWALKVAENELGITPVVSAQAVVAGSDPLGLIAYLSHFHSAF KSMAHSPGPVSQASPGTSSAVLFLSKLQRTLQRSRAKENAEDAGGKKLRLEMEAETPSTEVPPDPEPGVP LTPPSQHQEAGAGDLCALCGEHLYVLERLCVNGHFFHRSCFRCHTCEATLWPGGYEQHPGDGHFYCLQHL PQTDHKAEGSDRGPESPELPTPSENSMPPGLSTPTASQEGAGPVPDPSQPTRRQIRLSSPERQRLSSLNL TPDPEMEPPPKPPRSCSALARHALESSFVGWGLPVQSPQALVAMEKEEKESPFSSEEEEEDVPLDSDVEQ ALQTFAKTSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQTIQRRLNEIEAALRELEAEGVKLELALRRQSS SPEQQKKLWVGQLLQLVDKKNSLVAEEAELMITVQELNLEEKQWQLDQELRGYMNREENLKTAADRQAED QVLRKLVDLVNQRDALIRFQEERRLSELALGTGAQG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286613
ORF Size	3258 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286613.1, NP_001273542.1
RefSeq Size	3697 bp
RefSeq ORF	3261 bp
Locus ID	64780
UniProt ID	Q8TDZ2
MW	120.3 kDa
Gene Summary	This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

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