CD101 (NM_001256111) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EWI-101; IGSF2; V7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001256111, the custom clone sequence may differ by one or more nucleotides ATGGCAGGCATCTCATATGTGGCATCTTTCTTTCTCCTTCTGACTAAGCTCAGCATTGGCCAGAGAGAAG TAACAGTTCAGAAAGGACCACTGTTTAGAGCTGAAGGTTACCCAGTCAGCATTGGCTGCAATGTAACTGG CCACCAGGGACCTTCTGAGCAGCATTTCCAGTGGTCTGTTTACCTGCCGACAAACCCGACCCAGGAAGTC CAGATCATTAGCACCAAGGATGCTGCCTTCTCTTACGCAGTATATACGCAGCGGGTGCGAAGCGGAGACG TCTACGTGGAGAGGGTCCAGGGCAACTCAGTCTTGTTGCACATCTCAAAACTCCAGATGAAGGATGCTGG CGAGTATGAGTGTCACACACCAAACACTGATGAGAAATACTATGGAAGTTACAGTGCAAAGACTAATCTA ATTGATTTTATATTGGTCCCTGGGCCCTTGTATACAGAGCGGTTTGCAGCCAGTGACGTACAGCTCAACA AACTGGGACCCACTACATTCAGGCTGTCCATAGAGAGGCTCCAGTCCTCAGATCAGGGTCAGCTGTTCTG TGAGGCAACGGAATGGATTCAGGATCCAGATGAAACTTGGATGTTCATCACCAAAAAGCAGACCGATCAA ACCACTCTGAGGATCCAGCCAGCAGTGAAAGATTTTCAAGTCAACATTACAGCTGACAGCTTGTTTGCTG AAGGGAAACCCTTAGAACTGGTTTGCCTGGTTGTAAGCAGTGGCCGTGACCCACAGCTTCAAGGCATTTG GTTCTTCAATGGGACTGAAATTGCTCACATTGATGCTGGTGGAGTCCTGGGCCTGAAGAATGACTACAAA GAGAGAGCAAGTCAAGGAGAGCTCCAGGTTTCAAAGTTAGGCCCCAAGGCTTTCTCTCTCAAGATCTTCT CTCTGGGCCCAGAGGATGAAGGCGCCTACAGATGTGTGGTAGCAGAGGTCATGAAAACACGCACAGGTTC CTGGCAGGTGCTTCAGAGAAAGCAGTCACCAGACAGCCACGTGCACCTGAGGAAGCCAGCAGCAAGAAGT GTGGTCATGTCTACCAAGAACAAGCAGCAAGTTGTGTGGGAAGGAGAGACACTCGCCTTTCTCTGTAAGG CTGGTGGAGCTGAAAGTCCCCTGTCTGTGAGCTGGTGGCACATCCCACGGGACCAGACACAGCCCGAGTT TGTGGCTGGCATGGGGCAGGATGGCATTGTGCAGCTGGGTGCCTCCTATGGGGTACCCAGTTACCATGGC AACACAAGGCTGGAGAAAATGGACTGGGCCACCTTCCAGCTGGAGATCACCTTCACTGCCATCACAGACA GTGGCACATATGAGTGCAGAGTATCTGAGAAGTCTCGGAACCAGGCCAGAGATCTGAGCTGGACTCAGAA GATTTCAGTTACTGTAAAGTCTCTGGAGTCAAGTTTACAAGTTAGTCTGATGAGCCGTCAGCCGCAAGTG ATGTTAACCAACACCTTTGACCTGTCCTGTGTCGTGAGGGCCGGTTACTCTGACCTCAAGGTGCCACTCA CTGTGACGTGGCAGTTCCAGCCAGCTAGCTCTCACATCTTCCACCAGCTTATTCGAATCACCCACAATGG CACTATTGAATGGGGGAATTTCCTATCCCGGTTCCAAAAGAAGACGAAAGTGTCGCAGTCTTTATTTCGT TCACAACTCCTAGTCCATGATGCCACTGAGGAAGAGACAGGAGTGTATCAGTGTGAAGTAGAAGTTTATG ACAGAAATTCCCTATACAACAACCGCCCCCCGAGGGCTTCTGCCATCTCTCACCCACTGAGGATAGCCGT CACTTTACCAGAGAGCAAGCTAAAAGTGAATTCAAGGAGTCAAGTCCAAGAGCTCTCCATCAACTCCAAC ACTGATATAGAATGTAGCATCTTGTCCCGGTCCAATGGAAACCTTCAGTTAGCCATTATTTGGTATTTTT CTCCTGTTTCCACTAATGCCTCTTGGCTAAAGATCCTGGAGATGGACCAAACCAATGTTATAAAAACTGG GGATGAGTTTCACACCCCACAGAGAAAACAAAAATTTCATACTGAGAAGGTTTCCCAAGACTTATTTCAG CTGCACATTCTGAATGTGGAAGACAGCGATCGGGGCAAATATCACTGTGCTGTGGAGGAATGGCTCCTGT CTACAAATGGCACTTGGCACAAGCTTGGAGAAAAGAAGTCAGGACTAACAGAATTGAAACTCAAGCCCAC AGGAAGTAAGGTACGTGTCTCCAAAGTGTACTGGACCGAAAATGTGACTGAGCACAGAGAAGTGGCCATC CGCTGCAGCCTGGAGAGTGTAGGCAGCTCAGCCACTCTGTACTCTGTGATGTGGTACTGGAACAGAGAAA ACTCTGGAAGTAAATTGCTGGTGCACTTGCAACATGATGGCTTGCTGGAGTATGGGGAAGAGGGGCTCAG GAGGCACCTGCACTGTTACCGTTCATCCTCTACAGACTTTGTCCTGAAGCTTCATCAGGTGGAGATGGAG GATGCAGGAATGTACTGGTGTAGGGTGGCAGAGTGGCAGCTCCATGGACACCCAAGCAAGTGGATTAATC AAGCATCCGATGAGTCACAGCGGATGGTGCTCACGGTGCTGCCTTCAGAGCCCACGCTTCCTTCCAGGAT CTGCTCCTCGGCCCCTTTACTCTATTTCCTGTTCATCTGTCCCTTCGTCCTGCTCCTCCTTCTGCTCATC TCCCTCCTCTGCTTATACTGGAAGGCCAGGAAGTTGTCAACACTGCGTTCCAACACACGGAAAGAAAAAG CTCTCTGGGTGGACTTGAAAGAGGCTGGAGGTGTGACCACAAATAGGAGGGAAGACGAGGAGGAAGATGA AGGCAACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256111
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256111.2, NP_001243040.1
RefSeq Size	3334 bp
RefSeq ORF	2880 bp
Locus ID	9398
Protein Families	Transmembrane
Gene Summary	Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells. May be a marker of CD4(+) CD56(+) leukemic tumor cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 5' coding region, and also an alternate splice site in the 3' UTR, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.