CD101 (NM_001256111) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	EWI-101; IGSF2; V7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239827 representing NM_001256111 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGCATCTCATATGTGGCATCTTTCTTTCTCCTTCTGACTAAGCTCAGCATTGGCCAGAGAGAAG TAACAGTTCAGAAAGGACCACTGTTTAGAGCTGAAGGTTACCCAGTCAGCATTGGCTGCAATGTAACTGG CCACCAGGGACCTTCTGAGCAGCATTTCCAGTGGTCTGTTTACCTGCCGACAAACCCGACCCAGGAAGTC CAGATCATTAGCACCAAGGATGCTGCCTTCTCTTACGCAGTATATACGCAGCGGGTGCGAAGCGGAGACG TCTACGTGGAGAGGGTCCAGGGCAACTCAGTCTTGTTGCACATCTCAAAACTCCAGATGAAGGATGCTGG CGAGTATGAGTGTCACACACCAAACACTGATGAGAAATACTATGGAAGTTACAGTGCAAAGACTAATCTA ATTGATTTTATATTGGTCCCTGGGCCCTTGTATACAGAGCGGTTTGCAGCCAGTGACGTACAGCTCAACA AACTGGGACCCACTACATTCAGGCTGTCCATAGAGAGGCTCCAGTCCTCAGATCAGGGTCAGCTGTTCTG TGAGGCAACGGAATGGATTCAGGATCCAGATGAAACTTGGATGTTCATCACCAAAAAGCAGACCGATCAA ACCACTCTGAGGATCCAGCCAGCAGTGAAAGATTTTCAAGTCAACATTACAGCTGACAGCTTGTTTGCTG AAGGGAAACCCTTAGAACTGGTTTGCCTGGTTGTAAGCAGTGGCCGTGACCCACAGCTTCAAGGCATTTG GTTCTTCAATGGGACTGAAATTGCTCACATTGATGCTGGTGGAGTCCTGGGCCTGAAGAATGACTACAAA GAGAGAGCAAGTCAAGGAGAGCTCCAGGTTTCAAAGTTAGGCCCCAAGGCTTTCTCTCTCAAGATCTTCT CTCTGGGCCCAGAGGATGAAGGCGCCTACAGATGTGTGGTAGCAGAGGTCATGAAAACACGCACAGGTTC CTGGCAGGTGCTTCAGAGAAAGCAGTCACCAGACAGCCACGTGCACCTGAGGAAGCCAGCAGCAAGAAGT GTGGTCATGTCTACCAAGAACAAGCAGCAAGTTGTGTGGGAAGGAGAGACACTCGCCTTTCTCTGTAAGG CTGGTGGAGCTGAAAGTCCCCTGTCTGTGAGCTGGTGGCACATCCCACGGGACCAGACACAGCCCGAGTT TGTGGCTGGCATGGGGCAGGATGGCATTGTGCAGCTGGGTGCCTCCTATGGGGTACCCAGTTACCATGGC AACACAAGGCTGGAGAAAATGGACTGGGCCACCTTCCAGCTGGAGATCACCTTCACTGCCATCACAGACA GTGGCACATATGAGTGCAGAGTATCTGAGAAGTCTCGGAACCAGGCCAGAGATCTGAGCTGGACTCAGAA GATTTCAGTTACTGTAAAGTCTCTGGAGTCAAGTTTACAAGTTAGTCTGATGAGCCGTCAGCCGCAAGTG ATGTTAACCAACACCTTTGACCTGTCCTGTGTCGTGAGGGCCGGTTACTCTGACCTCAAGGTGCCACTCA CTGTGACGTGGCAGTTCCAGCCAGCTAGCTCTCACATCTTCCACCAGCTTATTCGAATCACCCACAATGG CACTATTGAATGGGGGAATTTCCTATCCCGGTTCCAAAAGAAGACGAAAGTGTCGCAGTCTTTATTTCGT TCACAACTCCTAGTCCATGATGCCACTGAGGAAGAGACAGGAGTGTATCAGTGTGAAGTAGAAGTTTATG ACAGAAATTCCCTATACAACAACCGCCCCCCGAGGGCTTCTGCCATCTCTCACCCACTGAGGATAGCCGT CACTTTACCAGAGAGCAAGCTAAAAGTGAATTCAAGGAGTCAAGTCCAAGAGCTCTCCATCAACTCCAAC ACTGATATAGAATGTAGCATCTTGTCCCGGTCCAATGGAAACCTTCAGTTAGCCATTATTTGGTATTTTT CTCCTGTTTCCACTAATGCCTCTTGGCTAAAGATCCTGGAGATGGACCAAACCAATGTTATAAAAACTGG GGATGAGTTTCACACCCCACAGAGAAAACAAAAATTTCATACTGAGAAGGTTTCCCAAGACTTATTTCAG CTGCACATTCTGAATGTGGAAGACAGCGATCGGGGCAAATATCACTGTGCTGTGGAGGAATGGCTCCTGT CTACAAATGGCACTTGGCACAAGCTTGGAGAAAAGAAGTCAGGACTAACAGAATTGAAACTCAAGCCCAC AGGAAGTAAGGTACGTGTCTCCAAAGTGTACTGGACCGAAAATGTGACTGAGCACAGAGAAGTGGCCATC CGCTGCAGCCTGGAGAGTGTAGGCAGCTCAGCCACTCTGTACTCTGTGATGTGGTACTGGAACAGAGAAA ACTCTGGAAGTAAATTGCTGGTGCACTTGCAACATGATGGCTTGCTGGAGTATGGGGAAGAGGGGCTCAG GAGGCACCTGCACTGTTACCGTTCATCCTCTACAGACTTTGTCCTGAAGCTTCATCAGGTGGAGATGGAG GATGCAGGAATGTACTGGTGTAGGGTGGCAGAGTGGCAGCTCCATGGACACCCAAGCAAGTGGATTAATC AAGCATCCGATGAGTCACAGCGGATGGTGCTCACGGTGCTGCCTTCAGAGCCCACGCTTCCTTCCAGGAT CTGCTCCTCGGCCCCTTTACTCTATTTCCTGTTCATCTGTCCCTTCGTCCTGCTCCTCCTTCTGCTCATC TCCCTCCTCTGCTTATACTGGAAGGCCAGGAAGTTGTCAACACTGCGTTCCAACACACGGAAAGAAAAAG CTCTCTGGGTGGACTTGAAAGAGGCTGGAGGTGTGACCACAAATAGGAGGGAAGACGAGGAGGAAGATGA AGGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239827 representing NM_001256111 Red=Cloning site Green=Tags(s) MAGISYVASFFLLLTKLSIGQREVTVQKGPLFRAEGYPVSIGCNVTGHQGPSEQHFQWSVYLPTNPTQEV QIISTKDAAFSYAVYTQRVRSGDVYVERVQGNSVLLHISKLQMKDAGEYECHTPNTDEKYYGSYSAKTNL IDFILVPGPLYTERFAASDVQLNKLGPTTFRLSIERLQSSDQGQLFCEATEWIQDPDETWMFITKKQTDQ TTLRIQPAVKDFQVNITADSLFAEGKPLELVCLVVSSGRDPQLQGIWFFNGTEIAHIDAGGVLGLKNDYK ERASQGELQVSKLGPKAFSLKIFSLGPEDEGAYRCVVAEVMKTRTGSWQVLQRKQSPDSHVHLRKPAARS VVMSTKNKQQVVWEGETLAFLCKAGGAESPLSVSWWHIPRDQTQPEFVAGMGQDGIVQLGASYGVPSYHG NTRLEKMDWATFQLEITFTAITDSGTYECRVSEKSRNQARDLSWTQKISVTVKSLESSLQVSLMSRQPQV MLTNTFDLSCVVRAGYSDLKVPLTVTWQFQPASSHIFHQLIRITHNGTIEWGNFLSRFQKKTKVSQSLFR SQLLVHDATEEETGVYQCEVEVYDRNSLYNNRPPRASAISHPLRIAVTLPESKLKVNSRSQVQELSINSN TDIECSILSRSNGNLQLAIIWYFSPVSTNASWLKILEMDQTNVIKTGDEFHTPQRKQKFHTEKVSQDLFQ LHILNVEDSDRGKYHCAVEEWLLSTNGTWHKLGEKKSGLTELKLKPTGSKVRVSKVYWTENVTEHREVAI RCSLESVGSSATLYSVMWYWNRENSGSKLLVHLQHDGLLEYGEEGLRRHLHCYRSSSTDFVLKLHQVEME DAGMYWCRVAEWQLHGHPSKWINQASDESQRMVLTVLPSEPTLPSRICSSAPLLYFLFICPFVLLLLLLI SLLCLYWKARKLSTLRSNTRKEKALWVDLKEAGGVTTNRREDEEEDEGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256111
ORF Size	2877 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256111.2, NP_001243040.1
RefSeq Size	3334 bp
RefSeq ORF	2880 bp
Locus ID	9398
Protein Families	Transmembrane
MW	109.1 kDa
Gene Summary	Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells. May be a marker of CD4(+) CD56(+) leukemic tumor cells.[UniProtKB/Swiss-Prot Function]

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