GPRC6A (NM_001286355) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA86F4.3; GPCR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286355, the custom clone sequence may differ by one or more nucleotides ATGGCATTCTTAATTATACTAATTACCTGCTTTGTGATTATTCTTGCTACTTCACAGCCTTGCCAGACCC CTGATGACTTTGTGGCTGCCACTTCTCCGGGACATATCATAATTGGAGGTTTGTTTGCTATTCATGAAAA AATGTTGTCCTCAGAAGACTCTCCCAGACGACCACAAATCCAGGAGTGTGTTGGCTTTGAAATATCAGTT TTTCTTCAAACTCTTGCCATGATACACAGCATTGAGATGATCAACAATTCAACACTCTTACCTGGAGTCA AACTGGGGTATGAAATCTATGACACTTGTACAGAAGTCACAGTGGCAATGGCAGCCACTCTGAGGTTTCT TTCTAAATTCAACTGCTCCAGAGAAACTGTGGAGTTTAAGTGTGACTATTCCAGCTACATGCCAAGAGTT AAGGCTGTCATAGGTTCTGGGTACTCAGAAATAACTATGGCTGTCTCCAGGATGTTGAATTTACAGCTCA TGCCACAGGTGGGTTATGAATCAACTGCAGAAATCCTGAGTGACAAAATTCGCTTTCCTTCATTTTTACG GACTGTGCCCAGTGACTTCCATCAAATTAAAGCAATGGCTCACCTGATTCAGAAATCTGGTTGGAACTGG ATTGGCATCATAACCACAGATGATGACTATGGACGATTGGCTCTTAACACTTTTATAATTCAGGCTGAAG CAAATAACGTGTGCATAGCCTTCAAAGAGGTTCTTCCAGCCTTTCTTTCAGATAATACCATTGAAGTCAG AATCAATCGGACACTGAAGAAAATCATTTTAGAAGCCCAGGTTAATGTCATTGTGGTATTTCTGAGGCAA TTCCATGTTTTTGATCTCTTCAATAAAGCCATTGAAATGAATATAAATAAGATGTGGATTGCTAGTGATA ATTGGTCAACTGCCACCAAGATTACCACCATTCCTAATGTTAAAAAGATTGGCAAAGTTGTAGGGTTTGC CTTTAGAAGAGGGAATATATCCTCTTTCCATTCCTTTCTTCAAAATCTGCACTTGCTTCCCAGTGACAGT CACAAACTCTTACATGAATATGCCATGCATTTATCTGCCTGCGCATATGTCAAGGACACTGATTTGAGTC AATGCATATTCAATCATTCTCAAAGGACTTTGGCCTACAAGGCTAACAAGGCTATAGAAAGGAACTTCGT CATGAGAAATGACTTCCTCTGGGACTATGCTGAGCCAGGACTCATTCATAGTATTCAGCTTGCAGTGTTT GCCCTTGGTTATGCCATTCGGGATCTGTGTCAAGCTCGTGACTGTCAGAACCCCAACGCCTTTCAACCAT GGGAGCAAATTCAATCTAAATGCTCCAAGGAATGCAGTCCTGGGCAAATGAAGAAAACTACAAGAAGTCA ACACATCTGTTGCTATGAATGTCAGAACTGTCCTGAAAATCATTACACTAATCAGACAGATATGCCTCAC TGCCTTTTATGCAACAACAAAACTCACTGGGCCCCTGTTAGGAGCACTATGTGCTTTGAAAAGGAAGTGG AATATCTCAACTGGAATGACTCCTTGGCCATCCTACTCCTGATTCTCTCCCTACTGGGAATCATATTTGT TCTGGTTGTTGGCATAATATTTACAAGAAACCTGAACACACCTGTTGTGAAATCATCCGGGGGATTAAGA GTCTGCTATGTGATCCTTCTCTGTCATTTCCTCAATTTTGCCAGCACGAGCTTTTTCATTGGAGAACCAC AAGACTTCACATGTAAAACCAGGCAGACAATGTTTGGAGTGAGCTTTACTCTTTGCATCTCCTGCATTTT GACGAAGTCTCTGAAAATTTTGCTAGCCTTCAGCTTTGATCCCAAATTACAGAAATTTCTGAAGTGCCTC TATAGACCGATCCTTATTATCTTCACTTGCACGGGCATCCAGGTTGTCATTTGCACACTCTGGCTAATCT TTGCAGCACCTACTGTAGAGGTGAATGTCTCCTTGCCCAGAGTCATCATCCTGGAGTGTGAGGAGGGATC CATACTTGCATTTGGCACCATGCTGGGCTACATTGCCATCCTGGCCTTCATTTGCTTCATATTTGCTTTC AAAGGCAAATATGAGAATTACAATGAAGCCAAATTCATTACATTTGGCATGCTCATTTACTTCATAGCTT GGATCACATTCATCCCTATCTATGCTACCACATTTGGCAAATATGTACCAGCTGTGGAGATTATTGTCAT ATTAATATCTAACTATGGAATCCTGTATTGCACATTCATCCCCAAATGCTATGTTATTATTTGTAAGCAA GAGATTAACACAAAGTCTGCCTTTCTCAAGATGATCTACAGTTATTCTTCCCATAGTGTGAGCAGCATTG CCCTGAGTCCTGCTTCACTGGACTCCATGAGCGGCAATGTCACAATGACCAATCCCAGCTCTAGTGGCAA GTCTGCAACCTGGCAGAAAAGCAAAGATCTTCAGGCACAAGCATTTGCACACATATGCAGGGAAAATGCC ACAAGTGTATCTAAAACTTTGCCTCGAAAAAGAATGTCAAGTATATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001286355
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286355.1, NP_001273284.1
RefSeq Size	2669 bp
RefSeq ORF	2568 bp
Locus ID	222545
UniProt ID	Q5T6X5
Protein Families	Druggable Genome, Transmembrane
Gene Summary	Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]).[supplied by OMIM, Nov 2010] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.