GPRC6A (NM_001286355) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bA86F4.3; GPCR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239691 representing NM_001286355 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATTCTTAATTATACTAATTACCTGCTTTGTGATTATTCTTGCTACTTCACAGCCTTGCCAGACCC CTGATGACTTTGTGGCTGCCACTTCTCCGGGACATATCATAATTGGAGGTTTGTTTGCTATTCATGAAAA AATGTTGTCCTCAGAAGACTCTCCCAGACGACCACAAATCCAGGAGTGTGTTGGCTTTGAAATATCAGTT TTTCTTCAAACTCTTGCCATGATACACAGCATTGAGATGATCAACAATTCAACACTCTTACCTGGAGTCA AACTGGGGTATGAAATCTATGACACTTGTACAGAAGTCACAGTGGCAATGGCAGCCACTCTGAGGTTTCT TTCTAAATTCAACTGCTCCAGAGAAACTGTGGAGTTTAAGTGTGACTATTCCAGCTACATGCCAAGAGTT AAGGCTGTCATAGGTTCTGGGTACTCAGAAATAACTATGGCTGTCTCCAGGATGTTGAATTTACAGCTCA TGCCACAGGTGGGTTATGAATCAACTGCAGAAATCCTGAGTGACAAAATTCGCTTTCCTTCATTTTTACG GACTGTGCCCAGTGACTTCCATCAAATTAAAGCAATGGCTCACCTGATTCAGAAATCTGGTTGGAACTGG ATTGGCATCATAACCACAGATGATGACTATGGACGATTGGCTCTTAACACTTTTATAATTCAGGCTGAAG CAAATAACGTGTGCATAGCCTTCAAAGAGGTTCTTCCAGCCTTTCTTTCAGATAATACCATTGAAGTCAG AATCAATCGGACACTGAAGAAAATCATTTTAGAAGCCCAGGTTAATGTCATTGTGGTATTTCTGAGGCAA TTCCATGTTTTTGATCTCTTCAATAAAGCCATTGAAATGAATATAAATAAGATGTGGATTGCTAGTGATA ATTGGTCAACTGCCACCAAGATTACCACCATTCCTAATGTTAAAAAGATTGGCAAAGTTGTAGGGTTTGC CTTTAGAAGAGGGAATATATCCTCTTTCCATTCCTTTCTTCAAAATCTGCACTTGCTTCCCAGTGACAGT CACAAACTCTTACATGAATATGCCATGCATTTATCTGCCTGCGCATATGTCAAGGACACTGATTTGAGTC AATGCATATTCAATCATTCTCAAAGGACTTTGGCCTACAAGGCTAACAAGGCTATAGAAAGGAACTTCGT CATGAGAAATGACTTCCTCTGGGACTATGCTGAGCCAGGACTCATTCATAGTATTCAGCTTGCAGTGTTT GCCCTTGGTTATGCCATTCGGGATCTGTGTCAAGCTCGTGACTGTCAGAACCCCAACGCCTTTCAACCAT GGGAGCAAATTCAATCTAAATGCTCCAAGGAATGCAGTCCTGGGCAAATGAAGAAAACTACAAGAAGTCA ACACATCTGTTGCTATGAATGTCAGAACTGTCCTGAAAATCATTACACTAATCAGACAGATATGCCTCAC TGCCTTTTATGCAACAACAAAACTCACTGGGCCCCTGTTAGGAGCACTATGTGCTTTGAAAAGGAAGTGG AATATCTCAACTGGAATGACTCCTTGGCCATCCTACTCCTGATTCTCTCCCTACTGGGAATCATATTTGT TCTGGTTGTTGGCATAATATTTACAAGAAACCTGAACACACCTGTTGTGAAATCATCCGGGGGATTAAGA GTCTGCTATGTGATCCTTCTCTGTCATTTCCTCAATTTTGCCAGCACGAGCTTTTTCATTGGAGAACCAC AAGACTTCACATGTAAAACCAGGCAGACAATGTTTGGAGTGAGCTTTACTCTTTGCATCTCCTGCATTTT GACGAAGTCTCTGAAAATTTTGCTAGCCTTCAGCTTTGATCCCAAATTACAGAAATTTCTGAAGTGCCTC TATAGACCGATCCTTATTATCTTCACTTGCACGGGCATCCAGGTTGTCATTTGCACACTCTGGCTAATCT TTGCAGCACCTACTGTAGAGGTGAATGTCTCCTTGCCCAGAGTCATCATCCTGGAGTGTGAGGAGGGATC CATACTTGCATTTGGCACCATGCTGGGCTACATTGCCATCCTGGCCTTCATTTGCTTCATATTTGCTTTC AAAGGCAAATATGAGAATTACAATGAAGCCAAATTCATTACATTTGGCATGCTCATTTACTTCATAGCTT GGATCACATTCATCCCTATCTATGCTACCACATTTGGCAAATATGTACCAGCTGTGGAGATTATTGTCAT ATTAATATCTAACTATGGAATCCTGTATTGCACATTCATCCCCAAATGCTATGTTATTATTTGTAAGCAA GAGATTAACACAAAGTCTGCCTTTCTCAAGATGATCTACAGTTATTCTTCCCATAGTGTGAGCAGCATTG CCCTGAGTCCTGCTTCACTGGACTCCATGAGCGGCAATGTCACAATGACCAATCCCAGCTCTAGTGGCAA GTCTGCAACCTGGCAGAAAAGCAAAGATCTTCAGGCACAAGCATTTGCACACATATGCAGGGAAAATGCC ACAAGTGTATCTAAAACTTTGCCTCGAAAAAGAATGTCAAGTATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239691 representing NM_001286355 Red=Cloning site Green=Tags(s) MAFLIILITCFVIILATSQPCQTPDDFVAATSPGHIIIGGLFAIHEKMLSSEDSPRRPQIQECVGFEISV FLQTLAMIHSIEMINNSTLLPGVKLGYEIYDTCTEVTVAMAATLRFLSKFNCSRETVEFKCDYSSYMPRV KAVIGSGYSEITMAVSRMLNLQLMPQVGYESTAEILSDKIRFPSFLRTVPSDFHQIKAMAHLIQKSGWNW IGIITTDDDYGRLALNTFIIQAEANNVCIAFKEVLPAFLSDNTIEVRINRTLKKIILEAQVNVIVVFLRQ FHVFDLFNKAIEMNINKMWIASDNWSTATKITTIPNVKKIGKVVGFAFRRGNISSFHSFLQNLHLLPSDS HKLLHEYAMHLSACAYVKDTDLSQCIFNHSQRTLAYKANKAIERNFVMRNDFLWDYAEPGLIHSIQLAVF ALGYAIRDLCQARDCQNPNAFQPWEQIQSKCSKECSPGQMKKTTRSQHICCYECQNCPENHYTNQTDMPH CLLCNNKTHWAPVRSTMCFEKEVEYLNWNDSLAILLLILSLLGIIFVLVVGIIFTRNLNTPVVKSSGGLR VCYVILLCHFLNFASTSFFIGEPQDFTCKTRQTMFGVSFTLCISCILTKSLKILLAFSFDPKLQKFLKCL YRPILIIFTCTGIQVVICTLWLIFAAPTVEVNVSLPRVIILECEEGSILAFGTMLGYIAILAFICFIFAF KGKYENYNEAKFITFGMLIYFIAWITFIPIYATTFGKYVPAVEIIVILISNYGILYCTFIPKCYVIICKQ EINTKSAFLKMIYSYSSHSVSSIALSPASLDSMSGNVTMTNPSSSGKSATWQKSKDLQAQAFAHICRENA TSVSKTLPRKRMSSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286355
ORF Size	2565 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286355.1, NP_001273284.1
RefSeq Size	2669 bp
RefSeq ORF	2568 bp
Locus ID	222545
UniProt ID	Q5T6X5
Protein Families	Druggable Genome, Transmembrane
MW	97 kDa
Gene Summary	Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]).[supplied by OMIM, Nov 2010]

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