Uromucoid (UMOD) (NM_001278614) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ADMCKD2; ADTKD1; FJHN; HNFJ; HNFJ1; MCKD2; THGP; THP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337176 representing NM_001278614. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGCAGCCATCTCTGACTTGGATGCTGATGGTGGTGGTGGCCTCTTGGTTCATCACAACTGCAGCC ACTGACACCTCAGAAGCAAGAACAAAGTACAACTGCCCAGCCAGGTGGAGTTGGAGGACTCCGCAGAGA GGAGGCGACACTGAGCAGGGTCCTGATGAAGATTTCACCAGCCAGGGAAGATGGTGCTCTGAATGTCAC AGCAATGCCACCTGCACGGAGGATGAGGCCGTTACGACGTGCACCTGTCAGGAGGGCTTCACCGGCGAT GGCCTGACCTGCGTGGACCTGGATGAGTGCGCCATTCCTGGAGCTCACAACTGCTCCGCCAACAGCAGC TGCGTAAACACGCCAGGCTCCTTCTCCTGCGTCTGCCCCGAAGGCTTCCGCCTGTCGCCCGGTCTCGGC TGCACAGACGTGGATGAGTGCGCTGAGCCTGGGCTTAGCCACTGCCACGCCCTGGCCACATGTGTCAAT GTGGTGGGCAGCTACTTGTGCGTATGCCCCGCGGGCTACCGGGGGGATGGATGGCACTGTGAGTGCTCC CCGGGCTCCTGCGGGCCGGGGTTGGACTGCGTGCCCGAGGGCGACGCGCTCGTGTGCGCGGATCCGTGC CAGGCGCACCGCACCCTGGACGAGTACTGGCGCAGCACCGAGTACGGGGAGGGCTACGCCTGCGACACG GACCTGCGCGGCTGGTACCGCTTCGTGGGCCAGGGCGGTGCGCGCATGGCCGAGACCTGCGTGCCAGTC CTGCGCTGCAACACGGCCGCCCCCATGTGGCTCAATGGCACGCATCCGTCCAGCGACGAGGGCATCGTG AGCCGCAAGGCCTGCGCGCACTGGAGCGGCCACTGCTGCCTGTGGGATGCGTCCGTCCAGGTGAAGGCC TGTGCCGGCGGCTACTACGTCTACAACCTGACAGCGCCCCCCGAGTGTCACCTGGCGTACTGCACAGAC CCCAGCTCCGTGGAGGGGACGTGTGAGGAGTGCAGTATAGACGAGGACTGCAAATCGAATAATGGCAGA TGGCACTGCCAGTGCAAACAGGACTTCAACATCACTGATATCTCCCTCCTGGAGCACAGGCTGGAATGT GGGGCCAATGACATGAAGGTGTCGCTGGGCAAGTGCCAGCTGAAGAGTCTGGGCTTCGACAAGGTCTTC ATGTACCTGAGTGACAGCCGGTGCTCGGGCTTCAATGACAGAGACAACCGGGACTGGGTGTCTGTAGTG ACCCCAGCCCGGGATGGCCCCTGTGGGACAGTGTTGACGAGGAATGAAACCCATGCCACTTACAGCAAC ACCCTCTACCTGGCAGATGAGATCATCATCCGTGACCTCAACATCAAAATCAACTTTGCATGCTCCTAC CCCCTGGACATGAAAGTCAGCCTGAAGACCGCCCTACAGCCAATGGTCAGTGCTCTAAACATCAGAGTG GGCGGGACCGGCATGTTCACCGTGCGGATGGCGCTCTTCCAGACCCCTTCCTACACGCAGCCCTACCAA GGCTCCTCCGTGACACTGTCCACTGAGGCTTTTCTCTACGTGGGCACCATGTTGGATGGGGGCGACCTG TCCCGATTTGCACTGCTCATGACCAACTGCTATGCCACACCCAGTAGCAATGCCACGGACCCCCTGAAG TACTTCATCATCCAGGACAGATGCCCACACACTAGAGACTCAACTATCCAAGTGGTGGAGAATGGGGAG TCCTCCCAGGGCCGATTTTCCGTCCAGATGTTCCGGTTTGCTGGAAACTATGACCTAGTCTACCTGCAC TGTGAAGTCTATCTCTGTGACACCATGAATGAAAAGTGCAAGCCTACCTGCTCTGGGACCAGATTCCGA AGTGGGAGTGTCATAGATCAATCCCGTGTCCTGAACTTGGGTCCCATCACACGGAAAGGTGTCCAGGCC ACAGTCTCAAGGGCTTTTAGCAGCTTGGGGCTCCTGAAAGTCTGGCTGCCTCTGCTTCTCTCGGCCACC TTGACCCTGACTTTTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278614
Insert Size	2022 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278614.1
RefSeq Size	2589 bp
RefSeq ORF	2022 bp
Locus ID	7369
UniProt ID	P07911
MW	73.6 kDa
Gene Summary	The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (3) includes an additional in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is longer than isoform a.

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