Uromucoid (UMOD) (NM_001278614) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ADMCKD2; ADTKD1; FJHN; HNFJ; HNFJ1; MCKD2; THGP; THP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239282 representing NM_001278614 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAGCCATCTCTGACTTGGATGCTGATGGTGGTGGTGGCCTCTTGGTTCATCACAACTGCAGCCA CTGACACCTCAGAAGCAAGAACAAAGTACAACTGCCCAGCCAGGTGGAGTTGGAGGACTCCGCAGAGAGG AGGCGACACTGAGCAGGGTCCTGATGAAGATTTCACCAGCCAGGGAAGATGGTGCTCTGAATGTCACAGC AATGCCACCTGCACGGAGGATGAGGCCGTTACGACGTGCACCTGTCAGGAGGGCTTCACCGGCGATGGCC TGACCTGCGTGGACCTGGATGAGTGCGCCATTCCTGGAGCTCACAACTGCTCCGCCAACAGCAGCTGCGT AAACACGCCAGGCTCCTTCTCCTGCGTCTGCCCCGAAGGCTTCCGCCTGTCGCCCGGTCTCGGCTGCACA GACGTGGATGAGTGCGCTGAGCCTGGGCTTAGCCACTGCCACGCCCTGGCCACATGTGTCAATGTGGTGG GCAGCTACTTGTGCGTATGCCCCGCGGGCTACCGGGGGGATGGATGGCACTGTGAGTGCTCCCCGGGCTC CTGCGGGCCGGGGTTGGACTGCGTGCCCGAGGGCGACGCGCTCGTGTGCGCGGATCCGTGCCAGGCGCAC CGCACCCTGGACGAGTACTGGCGCAGCACCGAGTACGGGGAGGGCTACGCCTGCGACACGGACCTGCGCG GCTGGTACCGCTTCGTGGGCCAGGGCGGTGCGCGCATGGCCGAGACCTGCGTGCCAGTCCTGCGCTGCAA CACGGCCGCCCCCATGTGGCTCAATGGCACGCATCCGTCCAGCGACGAGGGCATCGTGAGCCGCAAGGCC TGCGCGCACTGGAGCGGCCACTGCTGCCTGTGGGATGCGTCCGTCCAGGTGAAGGCCTGTGCCGGCGGCT ACTACGTCTACAACCTGACAGCGCCCCCCGAGTGTCACCTGGCGTACTGCACAGACCCCAGCTCCGTGGA GGGGACGTGTGAGGAGTGCAGTATAGACGAGGACTGCAAATCGAATAATGGCAGATGGCACTGCCAGTGC AAACAGGACTTCAACATCACTGATATCTCCCTCCTGGAGCACAGGCTGGAATGTGGGGCCAATGACATGA AGGTGTCGCTGGGCAAGTGCCAGCTGAAGAGTCTGGGCTTCGACAAGGTCTTCATGTACCTGAGTGACAG CCGGTGCTCGGGCTTCAATGACAGAGACAACCGGGACTGGGTGTCTGTAGTGACCCCAGCCCGGGATGGC CCCTGTGGGACAGTGTTGACGAGGAATGAAACCCATGCCACTTACAGCAACACCCTCTACCTGGCAGATG AGATCATCATCCGTGACCTCAACATCAAAATCAACTTTGCATGCTCCTACCCCCTGGACATGAAAGTCAG CCTGAAGACCGCCCTACAGCCAATGGTCAGTGCTCTAAACATCAGAGTGGGCGGGACCGGCATGTTCACC GTGCGGATGGCGCTCTTCCAGACCCCTTCCTACACGCAGCCCTACCAAGGCTCCTCCGTGACACTGTCCA CTGAGGCTTTTCTCTACGTGGGCACCATGTTGGATGGGGGCGACCTGTCCCGATTTGCACTGCTCATGAC CAACTGCTATGCCACACCCAGTAGCAATGCCACGGACCCCCTGAAGTACTTCATCATCCAGGACAGATGC CCACACACTAGAGACTCAACTATCCAAGTGGTGGAGAATGGGGAGTCCTCCCAGGGCCGATTTTCCGTCC AGATGTTCCGGTTTGCTGGAAACTATGACCTAGTCTACCTGCACTGTGAAGTCTATCTCTGTGACACCAT GAATGAAAAGTGCAAGCCTACCTGCTCTGGGACCAGATTCCGAAGTGGGAGTGTCATAGATCAATCCCGT GTCCTGAACTTGGGTCCCATCACACGGAAAGGTGTCCAGGCCACAGTCTCAAGGGCTTTTAGCAGCTTGG GGCTCCTGAAAGTCTGGCTGCCTCTGCTTCTCTCGGCCACCTTGACCCTGACTTTTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239282 representing NM_001278614 Red=Cloning site Green=Tags(s) MGQPSLTWMLMVVVASWFITTAATDTSEARTKYNCPARWSWRTPQRGGDTEQGPDEDFTSQGRWCSECHS NATCTEDEAVTTCTCQEGFTGDGLTCVDLDECAIPGAHNCSANSSCVNTPGSFSCVCPEGFRLSPGLGCT DVDECAEPGLSHCHALATCVNVVGSYLCVCPAGYRGDGWHCECSPGSCGPGLDCVPEGDALVCADPCQAH RTLDEYWRSTEYGEGYACDTDLRGWYRFVGQGGARMAETCVPVLRCNTAAPMWLNGTHPSSDEGIVSRKA CAHWSGHCCLWDASVQVKACAGGYYVYNLTAPPECHLAYCTDPSSVEGTCEECSIDEDCKSNNGRWHCQC KQDFNITDISLLEHRLECGANDMKVSLGKCQLKSLGFDKVFMYLSDSRCSGFNDRDNRDWVSVVTPARDG PCGTVLTRNETHATYSNTLYLADEIIIRDLNIKINFACSYPLDMKVSLKTALQPMVSALNIRVGGTGMFT VRMALFQTPSYTQPYQGSSVTLSTEAFLYVGTMLDGGDLSRFALLMTNCYATPSSNATDPLKYFIIQDRC PHTRDSTIQVVENGESSQGRFSVQMFRFAGNYDLVYLHCEVYLCDTMNEKCKPTCSGTRFRSGSVIDQSR VLNLGPITRKGVQATVSRAFSSLGLLKVWLPLLLSATLTLTFQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278614
ORF Size	2019 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278614.2
RefSeq Size	2589 bp
RefSeq ORF	2022 bp
Locus ID	7369
UniProt ID	P07911
MW	74 kDa
Gene Summary	The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2013]

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