ZNF143 (NM_001282657) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	pHZ-1; SBF; STAF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336962 representing NM_001282657. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTGTTAGCCCAAATAAATCGAGATTCTCAGGGAATGACAGAGTTTCCTGGAGGAGGGATGGAGGCG CAACATGTTACGCTGTGCTTGACAGAGGCAGTCACCGTGGCAGATGCAAAACTCATAGATGGCCAGGTC ATTCAGTTGGAAGATGGTTCTGCGGCCTATGTTCAACATGTACCCATACCTAAAAGTACAGGGGACAGT TTGCGTCTAGAGGATGGTCAAGCAGTACAGTTAGAAGATGGTACCACAGCATTTATTCACCACACCTCC AAAGATAGTTATGACCAGAGTGCATTACAGGCGGTTCAGCTGGAAGATGGTACCACAGCTTATATCCAC CATGCAGTGCAAGTCCCGCAGTCTGACACCATCTTGGCAATTCAGGCTGATGGGACAGTGGCAGGTCTG CACACTGGGGATGCTACAATTGACCCTGACACCATCAGTGCTTTGGAACAGTATGCAGCAAAGGTGTCC ATTGATGGAAGTGAAAGTGTAGCAGGTACTGGAATGATTGGAGAAAATGAGCAAGAGAAAAAAATGCAG ATTGTTTTACAAGGACATGCTACAAGAGTAACTGCTAAATCTCAACAGAGTGGAGAGAAGGCATTTCGA TGTGAATATGATGGATGTGGAAAATTATATACAACAGCTCATCATCTCAAGGTCCATGAGAGGTCACAC ACAGGAGATCGGCCTTATCAGTGTGAGCATGCAGGCTGTGGGAAGGCATTTGCAACAGGTTATGGATTA AAAAGTCACGTCAGAACTCATACAGGAGAAAAGCCATATCGGTGTTCGGAAGATAATTGTACTAAATCT TTCAAAACTTCAGGAGATCTACAGAAACACATCAGAACTCATACAGGAGAAAGGCCCTTTAAGTGTCCC TTCGAAGGCTGCGGTCGGTCCTTTACAACATCAAATATCAGAAAAGTGCACGTTAGGACACACACAGGA GAAAGACCTTATTACTGCACAGAGCCAGGATGTGGGAGGGCATTTGCCAGTGCAACAAATTATAAAAAC CATGTGAGGATACACACAGGAGAAAAGCCATATGTTTGTACAGTTCCTGGGTGTGACAAAAGGTTTACA GAATATTCCAGTTTGTACAAACATCATGTTGTCCACACTCATTCCAAACCTTACAACTGTAACCACTGT GGGAAGACATACAAGCAGATCTCCACGCTGGCCATGCACAAACGGACAGCCCACAACGACACTGAGCCC ATCGAGGAGGAGCAGGAAGCCTTCTTTGAGCCGCCCCCAGGTCAAGGTGAAGATGTTCTTAAAGGGTCC CAGATTACGTATGTTACAGGTGTAGAAGGGGACGACGTTGTTTCTACACAAGTAGCCACAGTAACCCAA TCTGGACTGAGTCAACAAGTTACACTCATATCCCAGGATGGGACTCAGCATGTCAACATATCTCAAGCT GACATGCAGGCCATTGGCAACACCATCACAATGGTAACGCAGGATGGCACGCCCATCACAGTCCCCGCC CATGATGCAGTCATCTCCTCAGCAGGAACGCACTCTGTTGCTATGGTTACTGCTGAGGGTACAGAAGGG GAACAGGTTGCAATTGTAGCTCAAGACTTGGCAGCATTCCATACTGCCTCATCAGAAATGGGGCACCAG CAGCATAGCCATCACTTAGTAACCACAGAAACCAGACCTCTGACCTTAGTAGCAACATCCAATGGCACC CAGATTGCAGTTCAGCTTGGAGAACAGCCATCTCTGGAAGAAGCCATCAGAATAGCGTCTAGAATCCAA CAAGGAGAAACGCCAGGGTTGGATGATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282657
Insert Size	1824 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282657.1
RefSeq Size	2870 bp
RefSeq ORF	1824 bp
Locus ID	7702
UniProt ID	P52747
Protein Families	Transcription Factors
MW	65.6 kDa
Gene Summary	Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1.

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