ZNF143 (NM_001282657) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	pHZ-1; SBF; STAF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239068 representing NM_001282657 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTTAGCCCAAATAAATCGAGATTCTCAGGGAATGACAGAGTTTCCTGGAGGAGGGATGGAGGCGC AACATGTTACGCTGTGCTTGACAGAGGCAGTCACCGTGGCAGATGCAAAACTCATAGATGGCCAGGTCAT TCAGTTGGAAGATGGTTCTGCGGCCTATGTTCAACATGTACCCATACCTAAAAGTACAGGGGACAGTTTG CGTCTAGAGGATGGTCAAGCAGTACAGTTAGAAGATGGTACCACAGCATTTATTCACCACACCTCCAAAG ATAGTTATGACCAGAGTGCATTACAGGCGGTTCAGCTGGAAGATGGTACCACAGCTTATATCCACCATGC AGTGCAAGTCCCGCAGTCTGACACCATCTTGGCAATTCAGGCTGATGGGACAGTGGCAGGTCTGCACACT GGGGATGCTACAATTGACCCTGACACCATCAGTGCTTTGGAACAGTATGCAGCAAAGGTGTCCATTGATG GAAGTGAAAGTGTAGCAGGTACTGGAATGATTGGAGAAAATGAGCAAGAGAAAAAAATGCAGATTGTTTT ACAAGGACATGCTACAAGAGTAACTGCTAAATCTCAACAGAGTGGAGAGAAGGCATTTCGATGTGAATAT GATGGATGTGGAAAATTATATACAACAGCTCATCATCTCAAGGTCCATGAGAGGTCACACACAGGAGATC GGCCTTATCAGTGTGAGCATGCAGGCTGTGGGAAGGCATTTGCAACAGGTTATGGATTAAAAAGTCACGT CAGAACTCATACAGGAGAAAAGCCATATCGGTGTTCGGAAGATAATTGTACTAAATCTTTCAAAACTTCA GGAGATCTACAGAAACACATCAGAACTCATACAGGAGAAAGGCCCTTTAAGTGTCCCTTCGAAGGCTGCG GTCGGTCCTTTACAACATCAAATATCAGAAAAGTGCACGTTAGGACACACACAGGAGAAAGACCTTATTA CTGCACAGAGCCAGGATGTGGGAGGGCATTTGCCAGTGCAACAAATTATAAAAACCATGTGAGGATACAC ACAGGAGAAAAGCCATATGTTTGTACAGTTCCTGGGTGTGACAAAAGGTTTACAGAATATTCCAGTTTGT ACAAACATCATGTTGTCCACACTCATTCCAAACCTTACAACTGTAACCACTGTGGGAAGACATACAAGCA GATCTCCACGCTGGCCATGCACAAACGGACAGCCCACAACGACACTGAGCCCATCGAGGAGGAGCAGGAA GCCTTCTTTGAGCCGCCCCCAGGTCAAGGTGAAGATGTTCTTAAAGGGTCCCAGATTACGTATGTTACAG GTGTAGAAGGGGACGACGTTGTTTCTACACAAGTAGCCACAGTAACCCAATCTGGACTGAGTCAACAAGT TACACTCATATCCCAGGATGGGACTCAGCATGTCAACATATCTCAAGCTGACATGCAGGCCATTGGCAAC ACCATCACAATGGTAACGCAGGATGGCACGCCCATCACAGTCCCCGCCCATGATGCAGTCATCTCCTCAG CAGGAACGCACTCTGTTGCTATGGTTACTGCTGAGGGTACAGAAGGGGAACAGGTTGCAATTGTAGCTCA AGACTTGGCAGCATTCCATACTGCCTCATCAGAAATGGGGCACCAGCAGCATAGCCATCACTTAGTAACC ACAGAAACCAGACCTCTGACCTTAGTAGCAACATCCAATGGCACCCAGATTGCAGTTCAGCTTGGAGAAC AGCCATCTCTGGAAGAAGCCATCAGAATAGCGTCTAGAATCCAACAAGGAGAAACGCCAGGGTTGGATGA T ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239068 representing NM_001282657 Red=Cloning site Green=Tags(s) MLLAQINRDSQGMTEFPGGGMEAQHVTLCLTEAVTVADAKLIDGQVIQLEDGSAAYVQHVPIPKSTGDSL RLEDGQAVQLEDGTTAFIHHTSKDSYDQSALQAVQLEDGTTAYIHHAVQVPQSDTILAIQADGTVAGLHT GDATIDPDTISALEQYAAKVSIDGSESVAGTGMIGENEQEKKMQIVLQGHATRVTAKSQQSGEKAFRCEY DGCGKLYTTAHHLKVHERSHTGDRPYQCEHAGCGKAFATGYGLKSHVRTHTGEKPYRCSEDNCTKSFKTS GDLQKHIRTHTGERPFKCPFEGCGRSFTTSNIRKVHVRTHTGERPYYCTEPGCGRAFASATNYKNHVRIH TGEKPYVCTVPGCDKRFTEYSSLYKHHVVHTHSKPYNCNHCGKTYKQISTLAMHKRTAHNDTEPIEEEQE AFFEPPPGQGEDVLKGSQITYVTGVEGDDVVSTQVATVTQSGLSQQVTLISQDGTQHVNISQADMQAIGN TITMVTQDGTPITVPAHDAVISSAGTHSVAMVTAEGTEGEQVAIVAQDLAAFHTASSEMGHQQHSHHLVT TETRPLTLVATSNGTQIAVQLGEQPSLEEAIRIASRIQQGETPGLDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282657
ORF Size	1821 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282657.1, NP_001269586.1
RefSeq Size	2870 bp
RefSeq ORF	1824 bp
Locus ID	7702
UniProt ID	P52747
Protein Families	Transcription Factors
MW	66.1 kDa
Gene Summary	Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8.[UniProtKB/Swiss-Prot Function]

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