WDR48 (NM_001303402) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Bun62; P80; SPG60; UAF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336921 representing NM_001303402. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGTGTCAATCAGCACAAGTAATATCTGCTTCTTCTGACACGACAGTAAAAGTATGGAATGCACAC AAGGGATTTTGCATGTCAACATTAAGGACACATAAGGATTACGTAAAGGCCTTAGCATATGCCAAGGAT AAAGAACTAGTAGCATCAGCTGGGTTGGACAGACAAATATTCCTTTGGGATGTGAATACTCTAACAGCA TTGACTGCCTCAAATAACACTGTCACAACTTCTTCTTTAAGTGGAAACAAAGATTCCATTTATAGCCTG GCCATGAATCAACTGGGAACAATCATTGTATCAGGGTCCACTGAAAAGGTGTTACGGGTATGGGATCCA AGAACATGTGCAAAACTAATGAAGCTTAAAGGGCACACGGATAATGTGAAGGCATTGCTATTAAACAGA GATGGCACGCAATGCCTGTCAGGCAGTTCTGATGGGACAATTCGCCTTTGGTCCCTTGGCCAGCAGAGA TGTATAGCAACATACCGAGTCCATGATGAAGGTGTTTGGGCGCTGCAAGTCAATGATGCCTTCACACAT GTGTATTCTGGTGGAAGGGACAGGAAGATTTATTGTACAGACCTAAGAAACCCTGACATTCGGGTGCTA ATTTGTGAAGAAAAAGCACCAGTTCTCAAGATGGAGCTTGATAGATCAGCTGATCCTCCTCCTGCAATT TGGGTTGCAACAACTAAGTCTACAGTAAATAAATGGACTTTGAAAGGAATTCATAATTTTAGAGCCTCT GGAGATTATGACAATGACTGTACAAATCCTATAACACCTCTTTGTACACAACCTGACCAGGTTATTAAA GGGGGTGCTAGTATTATTCAGTGCCACATTCTTAATGATAAGAGACATATATTAACCAAAGATACCAAT AATAATGTGGCATATTGGGATGTATTGAAGGCATGTAAAGTTGAAGATCTGGGCAAAGTGGATTTTGAA GATGAAATTAAGAAAAGATTTAAAATGGTGTATGTGCCAAATTGGTTCTCAGTAGACTTAAAAACAGGG ATGTTAACTATTACTTTGGATGAAAGTGATTGTTTTGCTGCCTGGGTTTCTGCAAAAGATGCTGGTTTC AGCAGCCCTGATGGGTCAGATCCAAAATTGAATTTAGGAGGACTTTTACTCCAAGCACTCCTGGAATAT TGGCCTAGAACACATGTGAATCCAATGGATGAAGAGGAAAATGAAGTAAACCATGTAAATGGGGAGCAG GAGAACCGAGTGCAGAAGGGAAATGGATATTTTCAAGTGCCCCCACATACACCCGTGATCTTTGGTGAA GCTGGAGGTCGCACACTGTTCAGGCTGCTCTGCCGAGATTCCGGGGGTGAGACTGAGTCTATGCTTCTT AATGAAACAGTGCCACAATGGGTAATTGACATCACTGTGGATAAAAATATGCCCAAATTCAACAAAATT CCTTTCTACCTCCAACCTCATGCATCTTCAGGAGCAAAAACCTTAAAAAAAGATAGACTCTCTGCTAGT GACATGCTCCAAGTCCGAAAAGTTATGGAACATGTTTATGAAAAAATTATCAACTTGGATAATGAGTCT CAAACCACTAGCTCTTCTAATAATGAAAAACCAGGAGAACAGGAAAAAGAAGAAGATATTGCTGTGTTG GCAGAGGAGAAAATTGAACTTTTGTGCCAGGACCAGGTTTTGGATCCAAATATGGACCTTCGAACAGTG AAACACTTCATATGGAAGAGCGGTGGAGACCTCACCCTCCATTACCGTCAGAAGTCCACGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001303402
Insert Size	1788 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303402.1
RefSeq Size	3948 bp
RefSeq ORF	1788 bp
Locus ID	57599
UniProt ID	Q8TAF3
MW	66.7 kDa
Gene Summary	The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (2) lacks an exon in the 5' coding region and uses an alternate start codon, which results in a frameshift compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus compared to isoform 1.

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