WDR48 (NM_001303402) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Bun62; P80; SPG60; UAF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239027 representing NM_001303402 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGTCAATCAGCACAAGTAATATCTGCTTCTTCTGACACGACAGTAAAAGTATGGAATGCACACA AGGGATTTTGCATGTCAACATTAAGGACACATAAGGATTACGTAAAGGCCTTAGCATATGCCAAGGATAA AGAACTAGTAGCATCAGCTGGGTTGGACAGACAAATATTCCTTTGGGATGTGAATACTCTAACAGCATTG ACTGCCTCAAATAACACTGTCACAACTTCTTCTTTAAGTGGAAACAAAGATTCCATTTATAGCCTGGCCA TGAATCAACTGGGAACAATCATTGTATCAGGGTCCACTGAAAAGGTGTTACGGGTATGGGATCCAAGAAC ATGTGCAAAACTAATGAAGCTTAAAGGGCACACGGATAATGTGAAGGCATTGCTATTAAACAGAGATGGC ACGCAATGCCTGTCAGGCAGTTCTGATGGGACAATTCGCCTTTGGTCCCTTGGCCAGCAGAGATGTATAG CAACATACCGAGTCCATGATGAAGGTGTTTGGGCGCTGCAAGTCAATGATGCCTTCACACATGTGTATTC TGGTGGAAGGGACAGGAAGATTTATTGTACAGACCTAAGAAACCCTGACATTCGGGTGCTAATTTGTGAA GAAAAAGCACCAGTTCTCAAGATGGAGCTTGATAGATCAGCTGATCCTCCTCCTGCAATTTGGGTTGCAA CAACTAAGTCTACAGTAAATAAATGGACTTTGAAAGGAATTCATAATTTTAGAGCCTCTGGAGATTATGA CAATGACTGTACAAATCCTATAACACCTCTTTGTACACAACCTGACCAGGTTATTAAAGGGGGTGCTAGT ATTATTCAGTGCCACATTCTTAATGATAAGAGACATATATTAACCAAAGATACCAATAATAATGTGGCAT ATTGGGATGTATTGAAGGCATGTAAAGTTGAAGATCTGGGCAAAGTGGATTTTGAAGATGAAATTAAGAA AAGATTTAAAATGGTGTATGTGCCAAATTGGTTCTCAGTAGACTTAAAAACAGGGATGTTAACTATTACT TTGGATGAAAGTGATTGTTTTGCTGCCTGGGTTTCTGCAAAAGATGCTGGTTTCAGCAGCCCTGATGGGT CAGATCCAAAATTGAATTTAGGAGGACTTTTACTCCAAGCACTCCTGGAATATTGGCCTAGAACACATGT GAATCCAATGGATGAAGAGGAAAATGAAGTAAACCATGTAAATGGGGAGCAGGAGAACCGAGTGCAGAAG GGAAATGGATATTTTCAAGTGCCCCCACATACACCCGTGATCTTTGGTGAAGCTGGAGGTCGCACACTGT TCAGGCTGCTCTGCCGAGATTCCGGGGGTGAGACTGAGTCTATGCTTCTTAATGAAACAGTGCCACAATG GGTAATTGACATCACTGTGGATAAAAATATGCCCAAATTCAACAAAATTCCTTTCTACCTCCAACCTCAT GCATCTTCAGGAGCAAAAACCTTAAAAAAAGATAGACTCTCTGCTAGTGACATGCTCCAAGTCCGAAAAG TTATGGAACATGTTTATGAAAAAATTATCAACTTGGATAATGAGTCTCAAACCACTAGCTCTTCTAATAA TGAAAAACCAGGAGAACAGGAAAAAGAAGAAGATATTGCTGTGTTGGCAGAGGAGAAAATTGAACTTTTG TGCCAGGACCAGGTTTTGGATCCAAATATGGACCTTCGAACAGTGAAACACTTCATATGGAAGAGCGGTG GAGACCTCACCCTCCATTACCGTCAGAAGTCCACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239027 representing NM_001303402 Red=Cloning site Green=Tags(s) MECQSAQVISASSDTTVKVWNAHKGFCMSTLRTHKDYVKALAYAKDKELVASAGLDRQIFLWDVNTLTAL TASNNTVTTSSLSGNKDSIYSLAMNQLGTIIVSGSTEKVLRVWDPRTCAKLMKLKGHTDNVKALLLNRDG TQCLSGSSDGTIRLWSLGQQRCIATYRVHDEGVWALQVNDAFTHVYSGGRDRKIYCTDLRNPDIRVLICE EKAPVLKMELDRSADPPPAIWVATTKSTVNKWTLKGIHNFRASGDYDNDCTNPITPLCTQPDQVIKGGAS IIQCHILNDKRHILTKDTNNNVAYWDVLKACKVEDLGKVDFEDEIKKRFKMVYVPNWFSVDLKTGMLTIT LDESDCFAAWVSAKDAGFSSPDGSDPKLNLGGLLLQALLEYWPRTHVNPMDEEENEVNHVNGEQENRVQK GNGYFQVPPHTPVIFGEAGGRTLFRLLCRDSGGETESMLLNETVPQWVIDITVDKNMPKFNKIPFYLQPH ASSGAKTLKKDRLSASDMLQVRKVMEHVYEKIINLDNESQTTSSSNNEKPGEQEKEEDIAVLAEEKIELL CQDQVLDPNMDLRTVKHFIWKSGGDLTLHYRQKST TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001303402
ORF Size	1785 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303402.2
RefSeq Size	3948 bp
RefSeq ORF	1788 bp
Locus ID	57599
UniProt ID	Q8TAF3
MW	67.2 kDa
Gene Summary	The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage. [provided by RefSeq, Sep 2016]

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