Smek1 (PPP4R3A) (NM_001284281) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLFL1; KIAA2010; MSTP033; PP4R3; PP4R3A; SMEK1; smk-1; smk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336919 representing NM_001284281. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCGACACCCGGCGGCGGGTGAAGGTGTACACGCTCAACGAGGACCGGCAGTGGGACGACCGGGGC ACCGGGCATGTGTCGTCTGGCTACGTGGAGCGGCTGAAGGGCATGTCCCTGCTTGTCAGGGCTGAGAGC GACGGTTCTCTACTTTTAGAGTCGAAAATAAATCCTAACACTGCATACCAGAAACAACAGGAAGATGAA AAATTTCTGACAGATTTGTTTGCACAACTAACAGATGAAGCAACAGATGAGGAAAAAAGACAGGAATTG GTTAACTTTTTAAAAGAATTTTGTGCGTTTTCCCAAACGCTACAGCCTCAAAACAGAGATGCTTTTTTC AAGACTTTGTCAAACATGGGCATATTACCAGCTTTAGAAGTCATCCTTGGCATGGATGATACACAGGTG CGAAGTGCTGCTACTGATATATTCTCATACTTGGTTGAATATAATCCATCCATGGTACGAGAGTTTGTC ATGCAGGAGGCACAACAGAATGATGATGTAAGTAAGAAGTTAACAGAGCAAAAAATAACCAGCAAGGAT ATTTTGCTCATCAACCTCATTATAGAACATATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAGTC CAGCTTATGGGCCTGCTTCGAACTTTAGTTGACCCAGAGAACATGCTAGCCACTGCCAATAAAACAGAA AAGACTGAATTTCTGGGTTTCTTCTACAAGCACTGTATGCATGTTCTCACTGCTCCTTTACTAGCAAAT ACAACAGAAGACAAACCTAGTAAAGATGATTTTCAGACTGCCCAACTATTGGCACTTGTATTGGAATTG TTAACATTTTGTGTGGAGCACCATACCTACCACATAAAGAACTACATTATTAATAAGGATATCCTCCGG AGAGTGCTAGTTCTTATGGCCTCGAAGCATGCTTTCTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAG ATTATTGGATTAAAAGATGAGTTTTACAACCGCTACATAATGAAAAGTTTTTTGTTTGAACCAGTAGTG AAAGCATTTCTCAACAATGGATCCCGCTACAATCTGATGAACTCTGCCATAATAGAGATGTTTGAATTT ATTAGAGTGGAAGATATAAAATCATTAACTGCTCATGTAATTGAAAATTACTGGAAAGCACTGGAAGAT GTAGATTATGTACAGACATTTAAAGGATTAAAACTGAGATTTGAACAACAAAGAGAAAGGCAAGATAAT CCCAAACTTGACAGTATGCGTTCCATTTTGAGGAATCACAGATATCGAAGAGATGCCAGAACACTAGAA GATGAAGAAGAGATGTGGTTTAACACAGATGAAGATGACATGGAAGATGGAGAAGCTGTAGTGTCTCCA TCTGACAAAACTAAAAATGATGATGATATTATGGATCCAATAAGTAAATTCATGGAAAGGAAGAAATTA AAAGAAAGTGAGGAAAAGGAAGTGCTTCTGAAAACAAACCTTTCTGGACGGCAGAGCCCAAGTTTCAAG CTTTCCCTGTCCAGTGGAACGAAGACTAACCTCACCAGCCAGTCATCTACAACAAATCTGCCTGGTTCT CCGGGATCACCTGGATCCCCAGGATCTCCAGGCTCTCCTGGATCCGTACCTAAAAATACATCTCAGACG GCAGCTATTACTACAAAGGGAGGCCTCGTGGGTCTGGTAGATTATCCTGATGATGATGAAGATGATGAT GAGGATGAAGATAAGGAAGATACGTTACCATTGTCAAAGAAAGCAAAATTTGATTCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001284281
Insert Size	1785 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284281.1
RefSeq Size	3896 bp
RefSeq ORF	1785 bp
Locus ID	55671
UniProt ID	Q6IN85
MW	67.8 kDa
Gene Summary	Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks two alternate in-frame exons and uses an alternate in-frame splice site, compared to variant 3. The encoded isoform (3) is shorter than isoform 1.

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