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Smek1 (PPP4R3A) (NM_001284281) Human Tagged ORF Clone
CAT＃: RG239025
- TrueORF®
SMEK1 (tGFP-tagged) - Human SMEK homolog 1, suppressor of mek1 (Dictyostelium) (SMEK1), transcript variant 4
ORF Plasmid: DDK
"NM_001284281" in other vectors (2)
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CNY 6,270.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FLFL1; KIAA2010; MSTP033; PP4R3; PP4R3A; SMEK1; smk-1; smk1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239025 representing NM_001284281.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCGACACCCGGCGGCGGGTGAAGGTGTACACGCTCAACGAGGACCGGCAGTGGGACGACCGGGGC ACCGGGCATGTGTCGTCTGGCTACGTGGAGCGGCTGAAGGGCATGTCCCTGCTTGTCAGGGCTGAGAGC GACGGTTCTCTACTTTTAGAGTCGAAAATAAATCCTAACACTGCATACCAGAAACAACAGGAAGATGAA AAATTTCTGACAGATTTGTTTGCACAACTAACAGATGAAGCAACAGATGAGGAAAAAAGACAGGAATTG GTTAACTTTTTAAAAGAATTTTGTGCGTTTTCCCAAACGCTACAGCCTCAAAACAGAGATGCTTTTTTC AAGACTTTGTCAAACATGGGCATATTACCAGCTTTAGAAGTCATCCTTGGCATGGATGATACACAGGTG CGAAGTGCTGCTACTGATATATTCTCATACTTGGTTGAATATAATCCATCCATGGTACGAGAGTTTGTC ATGCAGGAGGCACAACAGAATGATGATGTAAGTAAGAAGTTAACAGAGCAAAAAATAACCAGCAAGGAT ATTTTGCTCATCAACCTCATTATAGAACATATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAGTC CAGCTTATGGGCCTGCTTCGAACTTTAGTTGACCCAGAGAACATGCTAGCCACTGCCAATAAAACAGAA AAGACTGAATTTCTGGGTTTCTTCTACAAGCACTGTATGCATGTTCTCACTGCTCCTTTACTAGCAAAT ACAACAGAAGACAAACCTAGTAAAGATGATTTTCAGACTGCCCAACTATTGGCACTTGTATTGGAATTG TTAACATTTTGTGTGGAGCACCATACCTACCACATAAAGAACTACATTATTAATAAGGATATCCTCCGG AGAGTGCTAGTTCTTATGGCCTCGAAGCATGCTTTCTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAG ATTATTGGATTAAAAGATGAGTTTTACAACCGCTACATAATGAAAAGTTTTTTGTTTGAACCAGTAGTG AAAGCATTTCTCAACAATGGATCCCGCTACAATCTGATGAACTCTGCCATAATAGAGATGTTTGAATTT ATTAGAGTGGAAGATATAAAATCATTAACTGCTCATGTAATTGAAAATTACTGGAAAGCACTGGAAGAT GTAGATTATGTACAGACATTTAAAGGATTAAAACTGAGATTTGAACAACAAAGAGAAAGGCAAGATAAT CCCAAACTTGACAGTATGCGTTCCATTTTGAGGAATCACAGATATCGAAGAGATGCCAGAACACTAGAA GATGAAGAAGAGATGTGGTTTAACACAGATGAAGATGACATGGAAGATGGAGAAGCTGTAGTGTCTCCA TCTGACAAAACTAAAAATGATGATGATATTATGGATCCAATAAGTAAATTCATGGAAAGGAAGAAATTA AAAGAAAGTGAGGAAAAGGAAGTGCTTCTGAAAACAAACCTTTCTGGACGGCAGAGCCCAAGTTTCAAG CTTTCCCTGTCCAGTGGAACGAAGACTAACCTCACCAGCCAGTCATCTACAACAAATCTGCCTGGTTCT CCGGGATCACCTGGATCCCCAGGATCTCCAGGCTCTCCTGGATCCGTACCTAAAAATACATCTCAGACG GCAGCTATTACTACAAAGGGAGGCCTCGTGGGTCTGGTAGATTATCCTGATGATGATGAAGATGATGAT GAGGATGAAGATAAGGAAGATACGTTACCATTGTCAAAGAAAGCAAAATTTGATTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239025
Blue=ORF Red=Cloning site Green=Tag(s) MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQEDE KFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQV RSAATDIFSYLVEYNPSMVREFVMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMICDTDPELGGAV QLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLEL LTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVV KAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDN PKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKL KESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQT AAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001284281 |
| ORF Size | 1782 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001284281.1, NP_001271210.1 |
| RefSeq Size | 3896 bp |
| RefSeq ORF | 1785 bp |
| Locus ID | 55671 |
| UniProt ID | Q6IN85 |
| MW | 68.3 kDa |
| Gene Summary | Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.[UniProtKB/Swiss-Prot Function] |
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