EXDL1 (EXD1) (NM_001286441) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EXDL1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336834 representing NM_001286441. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACCCCAGCAGTGACTACCATTTCCTCAGCCAGATTTTGTGGAAGAGGGTGAAACTCACATTGGTC TGTGGTGTCTTCGAGGGTGTGCTTCAGCATGTTGACCCTAATAAGATTGTTGTCCTGAAGAAAGTGAAG AATGTGGAGACAGGTCGAAGTGTCCCAGGAGTGAAGTTGTTTTTTGGGCATGAGATTGTGAATGTGGAA CTACTAGATGAAGTGGAACAAGGCTCAGTGAGAGCAAAAGCATCTTCTGTTAGTCTACATGCAGAAAGA ACCTGGATGGAGAAAATGAAAGTTGAAGACCTAAATGTATGTGAGCCTGCTTCTCCTGCCCCTGAAGCA CCAGCTACCTCTCTGCTGAATGACCTCAAGTACAGCCCATCAGAGGAAGAGGAGGTGACATACACAGTC ATTAATCAATTCCAGCAGAAGTTTGGTGCTGCGATACTCCATATCAAGAAGCAGAATGTCCTGAGTGTG GCAGCAGAAGGAGCGAATGTATGTCGCCATGGCAAACTGTGCTGGCTGCAGGTGGCCACAAATTGCCGA GTTTACTTATTTGACATTTTCCTTCTGGGAAGTCGAGCTTTCCACAATGGACTTCAGATGATACTAGAA GACAAGAGAATTTTGAAGGTTATCCATGATTGTCGTTGGCTTTCTGATTGCCTCTCTCATCAGTATGGA ATTTTGCTGAATAATGTCTTTGACACACAGGTAGCAGATGTACTTCAGTTTTCCATGGAAACGGGTGGC TATCTTCCAAACTGCATCACTACTTTGCAGGAGAGTTTAATCAAACACCTTCAAGTAGCCCCTAAATAT CTCTCCTTTCTAGAAAAGAGACAAAAACTAATTCAGGAAAATCCAGAAGTATGGTTCATCCGACCTGTT TCACCCTCTTTACTGAAAATTTTGGCCCTGGAAGCTACCTACCTGTTACCCCTTCGCTTGGCACTCCTA GATGAGATGATGTCTGACCTAACCACCCTGGTGGATGGTTACCTAAACACGTATCGCGAAGGGTCTGCA GACCGGCTTGGAGGCACTGAGCCTACATGTATGGAGCTGCCAGAGGAACTGCTTCAACTCAAGGACTTC CAGAAGCAGCGCAGGGAGAAAGCTGCAAGAGAATATAGGGTGAATGCACAGGGACTCCTGATAAGGACA GTGCTACAGCCAAAGAAATTAGTGACAGAGACAGCAGGGAAAGAGGAGAAAGTCAAAGGCTTCTTATTT GGTAAAAATTTTAGGATAGATAAAGCTCCAAGTTTTACATCTCAAGACTTTCACGGGGATGTGAATTTA CTGAAAGAAGAATCTTTGAATAAACAAGCTACAAATCCTCAACATCTACCTCCCACAGAGGAAGGGGAA ACCAGTGAGGATTCCAGTAACAAACTCATTTGCACAAAGTCAAAGGGGTCAGAGGACCAGAGAATAACT CAGAAAGAACACTTTATGACACCCAAACATGAGTTTCAGGCAAGTTTATCTTTGAAAGAGGAGACAGAA CAGTTATTGATGGTGGAAAACAAGGAAGATTTAAAATGCACAAAACAGGCTGTTTCAATGTCTTCCTTT CCTCAGGAAACCAGAGTGTCTCCAAGTGACACTTTTTATCCTATCAGAAAGACTGTGGTTTCCACACTC CCTCCCTGTCCAGCCTTGGAGAAGATCGATTCCTGGATAAGTCCTTTTCTAAATCTGCCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286441
Insert Size	1719 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286441.1
RefSeq Size	3264 bp
RefSeq ORF	1719 bp
Locus ID	161829
UniProt ID	Q8NHP7
MW	64.8 kDa
Gene Summary	RNA-binding component of the PET complex, a multiprotein complex required for the processing of piRNAs during spermatogenesis. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposable elements, preventing their mobilization, which is essential for the germline integrity (By similarity). The PET complex is required during the secondary piRNAs metabolic process for the PIWIL2 slicing-triggered loading of PIWIL4 piRNAs. In the PET complex, EXD1 probably acts as an RNA adapter. EXD1 is an inactive exonuclease (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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