ARSL (NM_001282631) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARSE; ASE; CDPX; CDPX1; CDPXR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336723 representing NM_001282631. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCAGGAAGTAGAGAGAGAGACAACATGTTACATCTGCACCATTCTTGGACTCCGAATATTGACCGC CTTGCAGAGGACGGCGTGAAGCTGACCCAACACATCTCTGCCGCATCTTTGTGCACCCCAAGCAGAGCC GCCTTCCTCACGGGCAGATACCCTGTGCGATCAGGGATGGTTTCCAGCATTGGTTACCGTGTTCTTCAG TGGACCGGAGCATCTGGAGGTCTTCCAACAAATGAGACAACTTTTGCAAAAATACTGAAAGAGAAAGGC TATGCCACTGGACTCATTGGAAAATGGCATCTGGGTCTCAACTGTGAGTCAGCCAGTGATCATTGCCAC CACCCTCTCCATCATGGCTTTGACCATTTCTACGGAATGCCTTTCTCCTTGATGGGTGATTGCGCCCGC TGGGAACTCTCAGAGAAGCGTGTCAACCTGGAACAAAAACTCAACTTCCTCTTCCAAGTCCTGGCCTTG GTTGCCCTCACACTGGTAGCAGGGAAGCTCACACACCTGATACCCGTCTCGTGGATGCCGGTCATCTGG TCAGCCCTTTCGGCCGTCCTCCTCCTCGCAAGCTCCTATTTTGTGGGTGCTCTGATTGTCCATGCCGAT TGCTTTCTGATGAGAAACCACACCATCACGGAGCAGCCCATGTGCTTCCAAAGAACGACACCCCTTATT CTGCAGGAGGTTGCGTCCTTTCTCAAAAGGAATAAGCATGGGCCTTTCCTCCTCTTTGTTTCCTTTCTA CACGTTCACATCCCTCTTATCACTATGGAGAACTTCCTCGGGAAGAGTCTCCACGGGCTGTATGGGGAC AACGTAGAGGAGATGGACTGGATGGTAGGACGGATCCTTGACACTTTGGACGTGGAGGGTTTGAGCAAC AGCACCCTCATTTATTTTACGTCGGATCACGGCGGTTCCCTAGAGAATCAACTTGGAAACACCCAGTAT GGTGGCTGGAATGGAATTTATAAAGGTGGGAAGGGCATGGGAGGATGGGAAGGTGGGATCCGCGTGCCC GGGATCTTCCGCTGGCCCGGGGTGCTCCCGGCCGGCCGAGTGATTGGCGAGCCCACGAGTCTGATGGAC GTGTTCCCCACCGTGGTCCGGCTGGCGGGCGGCGAGGTGCCCCAGGACAGAGTGATTGACGGCCAAGAC CTTCTGCCCTTGCTCCTGGGGACAGCCCAACACTCAGACCACGAGTTCCTGATGCATTATTGTGAGAGG TTTCTGCACGCAGCCAGGTGGCATCAACGGGACAGAGGAACAATGTGGAAAGTCCACTTTGTGACGCCT GTGTTCCAGCCAGAGGGAGCCGGTGCCTGCTATGGAAGAAAGGTCTGCCCGTGCTTTGGGGAAAAAGTA GTCCACCACGATCCACCTTTGCTCTTTGACCTCTCAAGAGACCCTTCTGAGACCCACATCCTCACACCA GCCTCAGAGCCCGTGTTCTATCAGGTGATGGAACGAGTCCAGCAGGCGGTGTGGGAACACCAGCGGACA CTCAGCCCAGTTCCTCTGCAGCTGGACAGGCTGGGCAACATCTGGAGACCGTGGCTGCAGCCCTGCTGT GGCCCGTTCCCCCTCTGCTGGTGCCTTAGGGAAGATGACCCACAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282631
Insert Size	1635 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282631.1
RefSeq Size	1990 bp
RefSeq ORF	1635 bp
Locus ID	415
Protein Families	Druggable Genome, Transmembrane
MW	61.1 kDa
Gene Summary	Arylsulfatase E is a member of the sulfatase family. It is glycosylated postranslationally and localized to the golgi apparatus. Sulfatases are essential for the correct composition of bone and cartilage matrix. X-linked chondrodysplasia punctata, a disease characterized by abnormalities in cartilage and bone development, has been linked to mutations in this gene. Alternative splicing results in multiple transcript variants. A pseudogene related to this gene is located on the Y chromosome. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (3) contains an alternate 5' terminal exon, lacks two alternate exons in the coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1.

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