ARSL (NM_001282631) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARSE; ASE; CDPX; CDPX1; CDPXR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238829 representing NM_001282631 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGGAAGTAGAGAGAGAGACAACATGTTACATCTGCACCATTCTTGGACTCCGAATATTGACCGCC TTGCAGAGGACGGCGTGAAGCTGACCCAACACATCTCTGCCGCATCTTTGTGCACCCCAAGCAGAGCCGC CTTCCTCACGGGCAGATACCCTGTGCGATCAGGGATGGTTTCCAGCATTGGTTACCGTGTTCTTCAGTGG ACCGGAGCATCTGGAGGTCTTCCAACAAATGAGACAACTTTTGCAAAAATACTGAAAGAGAAAGGCTATG CCACTGGACTCATTGGAAAATGGCATCTGGGTCTCAACTGTGAGTCAGCCAGTGATCATTGCCACCACCC TCTCCATCATGGCTTTGACCATTTCTACGGAATGCCTTTCTCCTTGATGGGTGATTGCGCCCGCTGGGAA CTCTCAGAGAAGCGTGTCAACCTGGAACAAAAACTCAACTTCCTCTTCCAAGTCCTGGCCTTGGTTGCCC TCACACTGGTAGCAGGGAAGCTCACACACCTGATACCCGTCTCGTGGATGCCGGTCATCTGGTCAGCCCT TTCGGCCGTCCTCCTCCTCGCAAGCTCCTATTTTGTGGGTGCTCTGATTGTCCATGCCGATTGCTTTCTG ATGAGAAACCACACCATCACGGAGCAGCCCATGTGCTTCCAAAGAACGACACCCCTTATTCTGCAGGAGG TTGCGTCCTTTCTCAAAAGGAATAAGCATGGGCCTTTCCTCCTCTTTGTTTCCTTTCTACACGTTCACAT CCCTCTTATCACTATGGAGAACTTCCTCGGGAAGAGTCTCCACGGGCTGTATGGGGACAACGTAGAGGAG ATGGACTGGATGGTAGGACGGATCCTTGACACTTTGGACGTGGAGGGTTTGAGCAACAGCACCCTCATTT ATTTTACGTCGGATCACGGCGGTTCCCTAGAGAATCAACTTGGAAACACCCAGTATGGTGGCTGGAATGG AATTTATAAAGGTGGGAAGGGCATGGGAGGATGGGAAGGTGGGATCCGCGTGCCCGGGATCTTCCGCTGG CCCGGGGTGCTCCCGGCCGGCCGAGTGATTGGCGAGCCCACGAGTCTGATGGACGTGTTCCCCACCGTGG TCCGGCTGGCGGGCGGCGAGGTGCCCCAGGACAGAGTGATTGACGGCCAAGACCTTCTGCCCTTGCTCCT GGGGACAGCCCAACACTCAGACCACGAGTTCCTGATGCATTATTGTGAGAGGTTTCTGCACGCAGCCAGG TGGCATCAACGGGACAGAGGAACAATGTGGAAAGTCCACTTTGTGACGCCTGTGTTCCAGCCAGAGGGAG CCGGTGCCTGCTATGGAAGAAAGGTCTGCCCGTGCTTTGGGGAAAAAGTAGTCCACCACGATCCACCTTT GCTCTTTGACCTCTCAAGAGACCCTTCTGAGACCCACATCCTCACACCAGCCTCAGAGCCCGTGTTCTAT CAGGTGATGGAACGAGTCCAGCAGGCGGTGTGGGAACACCAGCGGACACTCAGCCCAGTTCCTCTGCAGC TGGACAGGCTGGGCAACATCTGGAGACCGTGGCTGCAGCCCTGCTGTGGCCCGTTCCCCCTCTGCTGGTG CCTTAGGGAAGATGACCCACAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238829 representing NM_001282631 Red=Cloning site Green=Tags(s) MSGSRERDNMLHLHHSWTPNIDRLAEDGVKLTQHISAASLCTPSRAAFLTGRYPVRSGMVSSIGYRVLQW TGASGGLPTNETTFAKILKEKGYATGLIGKWHLGLNCESASDHCHHPLHHGFDHFYGMPFSLMGDCARWE LSEKRVNLEQKLNFLFQVLALVALTLVAGKLTHLIPVSWMPVIWSALSAVLLLASSYFVGALIVHADCFL MRNHTITEQPMCFQRTTPLILQEVASFLKRNKHGPFLLFVSFLHVHIPLITMENFLGKSLHGLYGDNVEE MDWMVGRILDTLDVEGLSNSTLIYFTSDHGGSLENQLGNTQYGGWNGIYKGGKGMGGWEGGIRVPGIFRW PGVLPAGRVIGEPTSLMDVFPTVVRLAGGEVPQDRVIDGQDLLPLLLGTAQHSDHEFLMHYCERFLHAAR WHQRDRGTMWKVHFVTPVFQPEGAGACYGRKVCPCFGEKVVHHDPPLLFDLSRDPSETHILTPASEPVFY QVMERVQQAVWEHQRTLSPVPLQLDRLGNIWRPWLQPCCGPFPLCWCLREDDPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282631
ORF Size	1632 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282631.1, NP_001269560.1
RefSeq Size	1990 bp
RefSeq ORF	1608 bp
Locus ID	415
Protein Families	Druggable Genome, Transmembrane
MW	61.5 kDa
Gene Summary	Arylsulfatase E is a member of the sulfatase family. It is glycosylated postranslationally and localized to the golgi apparatus. Sulfatases are essential for the correct composition of bone and cartilage matrix. X-linked chondrodysplasia punctata, a disease characterized by abnormalities in cartilage and bone development, has been linked to mutations in this gene. Alternative splicing results in multiple transcript variants. A pseudogene related to this gene is located on the Y chromosome. [provided by RefSeq, Sep 2013]

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