Deformed Epidermal Autoregulatory Factor 1 (DEAF1) (NM_001293634) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MRD24; NEDHELS; NUDR; SPN; VSVS; ZMYND5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336425 representing NM_001293634. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGACTCGGACTCGGCGGCAAAGCAGCTGGGCCTGGCTGAGGCGGCGGCGGTGGCGGCCGCGGCC GCTGTGGCGGCGGCGGCCGCGGCCGCGGCAGGAGGCGAGGCGGAGGAGCCGGTGCTGAGCAGGGACGAG GACTCGGAGGAGGACGCAGACTCGGAGGCGGAGCGGGAGACGCCGCGGGTCACGGCAGTGGCGGTGATG GCGGCGGAGCCCGGGCACATGGACATGGGCGCCGAGGCCCTGCCCGGCCCCGACGAGGCCGCCGCTGCC GCAGCCTTCGCAGAGGTGACCACAGTGACAGTGGCCAACGTGGGGGCTGCTGCAGACAATGTCTTCACC ACGTCTGTGGCGAACGCGGCATCCATCTCAGGACATGTTCTGTCTGGTAGGACGGCCCTTCAGATCGGG GACAGCCTGAACACCGAAAAAGCGACACTGATTGTCGTCCACACAGATGGGAGCATCGTGGAGACCACC GGGCTGAAAGGCCCGGCAGCTCCCCTCACCCCAGGTCCTCAGTCTCCTCCAACCCCTCTGGCTCCCGGC CAAGAAAAAGGTGGAACTAAATACAACTGGGACCCTTCTGTGTACGACAGTGAGCTGCCCGTACGGTGC CGGAACATCAGCGGCACTCTGTACAAGAACAGGCTCGGCTCAGGATGGGATCTTAAACCCTCACGCTGC CTCTTGCACCTGTGCTGCCTGCTGCGACGACATGACCTTATCACCGTGACCCCCTCGGGACAGATCACG ACCTCGGGGGCACTGACCTTTGACCGAGCGTCCACGGTAGAGGCCACTGCTGTCATATCAGAGAGTCCG GCCCAGGGCGACGTCTTCGCAGGGGCCACAGTCCAAGAGGCCAGCGTGCAGCCCCCATGCAGGGCCAGC CACCCTGAGCCTCACTACCCCGGCTATCAGGACAGCTGCCAGATCGCACCGTTTCCAGAAGCTGCGTTG CCAACGTCACATCCCAAAATAGTGTTGACATCCCTGCCTGCGCTGGCGGTCCCACCCCCGACTCCCACC AAAGCGGCACCTCCCGCGTTGGTCAATGGGCTGGAGCTGTCAGAGCCGCGGAGCTGGCTGTACCTAGAA GAGATGGTCAACTCCTTGCTCAACACAGCGCAGCAGCTGAAGACGCTGTTTGAGCAAGCCAAGCATGCC AGCACCTACCGAGAAGCTGCCACAAACCAGGCCAAGATCCACGCTGACGCAGAGCGGAAGGAGGTGATC CACCCGCCTCGGCTGCCCAAAGTCCTGGGATTACAGCAGTCCTGCGTTAACTGCGGCCGGGAGGCTATG AGCGAGTGCACCGGCTGCCACAAGGTCAACTACTGCTCCACCTTCTGCCAACGCAAGGACTGGAAGGAT CACCAGCACATATGCGGCCAGTCAGCAGCTGTCACCGTCCAGGCAGACGAAGTCCACGTGGCTGAAAGC GTGATGGAGAAGGTGACCGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001293634
Insert Size	1473 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293634.1
RefSeq Size	2523 bp
RefSeq ORF	1473 bp
Locus ID	10522
UniProt ID	O75398
Protein Families	Secreted Protein, Transcription Factors, Transmembrane
MW	51.3 kDa
Gene Summary	This gene encodes a zinc finger domain-containing protein that functions as a regulator of transcription. The encoded proteins binds to its own promoter as well as to that of several target genes. Activity of this protein is important in the regulation of embryonic development. Mutations in this gene have been found in individuals with autosomal dominant cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (2, also known as DF1-VAR) lacks two internal coding exons and contains an alternate coding exon, compared to variant 1. The encoded isoform (b) is shorter than isoform a.

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