Deformed Epidermal Autoregulatory Factor 1 (DEAF1) (NM_001293634) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MRD24; NEDHELS; NUDR; SPN; VSVS; ZMYND5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238531 representing NM_001293634 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACTCGGACTCGGCGGCAAAGCAGCTGGGCCTGGCTGAGGCGGCGGCGGTGGCGGCCGCGGCCG CTGTGGCGGCGGCGGCCGCGGCCGCGGCAGGAGGCGAGGCGGAGGAGCCGGTGCTGAGCAGGGACGAGGA CTCGGAGGAGGACGCAGACTCGGAGGCGGAGCGGGAGACGCCGCGGGTCACGGCAGTGGCGGTGATGGCG GCGGAGCCCGGGCACATGGACATGGGCGCCGAGGCCCTGCCCGGCCCCGACGAGGCCGCCGCTGCCGCAG CCTTCGCAGAGGTGACCACAGTGACAGTGGCCAACGTGGGGGCTGCTGCAGACAATGTCTTCACCACGTC TGTGGCGAACGCGGCATCCATCTCAGGACATGTTCTGTCTGGTAGGACGGCCCTTCAGATCGGGGACAGC CTGAACACCGAAAAAGCGACACTGATTGTCGTCCACACAGATGGGAGCATCGTGGAGACCACCGGGCTGA AAGGCCCGGCAGCTCCCCTCACCCCAGGTCCTCAGTCTCCTCCAACCCCTCTGGCTCCCGGCCAAGAAAA AGGTGGAACTAAATACAACTGGGACCCTTCTGTGTACGACAGTGAGCTGCCCGTACGGTGCCGGAACATC AGCGGCACTCTGTACAAGAACAGGCTCGGCTCAGGATGGGATCTTAAACCCTCACGCTGCCTCTTGCACC TGTGCTGCCTGCTGCGACGACATGACCTTATCACCGTGACCCCCTCGGGACAGATCACGACCTCGGGGGC ACTGACCTTTGACCGAGCGTCCACGGTAGAGGCCACTGCTGTCATATCAGAGAGTCCGGCCCAGGGCGAC GTCTTCGCAGGGGCCACAGTCCAAGAGGCCAGCGTGCAGCCCCCATGCAGGGCCAGCCACCCTGAGCCTC ACTACCCCGGCTATCAGGACAGCTGCCAGATCGCACCGTTTCCAGAAGCTGCGTTGCCAACGTCACATCC CAAAATAGTGTTGACATCCCTGCCTGCGCTGGCGGTCCCACCCCCGACTCCCACCAAAGCGGCACCTCCC GCGTTGGTCAATGGGCTGGAGCTGTCAGAGCCGCGGAGCTGGCTGTACCTAGAAGAGATGGTCAACTCCT TGCTCAACACAGCGCAGCAGCTGAAGACGCTGTTTGAGCAAGCCAAGCATGCCAGCACCTACCGAGAAGC TGCCACAAACCAGGCCAAGATCCACGCTGACGCAGAGCGGAAGGAGGTGATCCACCCGCCTCGGCTGCCC AAAGTCCTGGGATTACAGCAGTCCTGCGTTAACTGCGGCCGGGAGGCTATGAGCGAGTGCACCGGCTGCC ACAAGGTCAACTACTGCTCCACCTTCTGCCAACGCAAGGACTGGAAGGATCACCAGCACATATGCGGCCA GTCAGCAGCTGTCACCGTCCAGGCAGACGAAGTCCACGTGGCTGAAAGCGTGATGGAGAAGGTGACCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238531 representing NM_001293634 Red=Cloning site Green=Tags(s) MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAGGEAEEPVLSRDEDSEEDADSEAERETPRVTAVAVMA AEPGHMDMGAEALPGPDEAAAAAAFAEVTTVTVANVGAAADNVFTTSVANAASISGHVLSGRTALQIGDS LNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNI SGTLYKNRLGSGWDLKPSRCLLHLCCLLRRHDLITVTPSGQITTSGALTFDRASTVEATAVISESPAQGD VFAGATVQEASVQPPCRASHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPPTPTKAAPP ALVNGLELSEPRSWLYLEEMVNSLLNTAQQLKTLFEQAKHASTYREAATNQAKIHADAERKEVIHPPRLP KVLGLQQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHICGQSAAVTVQADEVHVAESVMEKVTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293634
ORF Size	1470 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293634.1, NP_001280563.1
RefSeq Size	2523 bp
RefSeq ORF	1473 bp
Locus ID	10522
UniProt ID	O75398
Protein Families	Secreted Protein, Transcription Factors, Transmembrane
MW	51.7 kDa
Gene Summary	This gene encodes a zinc finger domain-containing protein that functions as a regulator of transcription. The encoded proteins binds to its own promoter as well as to that of several target genes. Activity of this protein is important in the regulation of embryonic development. Mutations in this gene have been found in individuals with autosomal dominant cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

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