Septin 2 (SEPT2) (NM_001282973) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DIFF6; hNedd5; NEDD-5; NEDD5; Pnutl3; SEPT2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335687 representing NM_001282973. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTAAGCAACAGCCAACTCAGTTTATAAATCCAGAAACACCTGGCTATGTTGGATTTGCAAACCTC CCCAATCAAGTTCACCGAAAATCAGTGAAAAAAGGTTTTGAGTTCACACTGATGGTGGTCGGTGAATCA GGTCTAGGAAAATCGACTCTCATAAACAGCCTATTCCTAACTGATCTGTACCCAGAAAGAGTCATACCT GGAGCAGCAGCTTTGAACACAAGGAAAACACTTTTATGGGAAAAAATTGAAAGAACTGTCCAGATTGAG GCTTCAACTGTTGAAATTGAAGAGCGAGGGGTCAAGCTACGCCTGACAGTGGTAGATACCCCTGGCTAT GGTGACGCTATCAACTGCAGAGATTGTTTTAAGACAATTATCTCCTATATTGATGAGCAATTTGAGAGG TACCTGCATGACGAGAGCGGCTTGAACAGGCGGCACATCATTGATAATAGGGTGCATTGTTGCTTTTAC TTTATTTCACCTTTTGGACATGGACTTAAGCCCTTAGATGTGGCGTTTATGAAGGCAATACACAACAAG GTGAATATTGTGCCTGTCATTGCAAAAGCTGACACTCTCACCCTGAAGGAACGGGAGCGGCTGAAGAAA AGGATTCTGGATGAAATTGAAGAACATAACATCAAAATCTATCACTTACCTGATGCAGAATCAGATGAA GATGAAGATTTTAAAGAGCAGACTAGACTTCTCAAGGCTAGCATCCCATTCTCTGTGGTTGGATCCAAT CAGTTGATTGAAGCCAAAGGAAAGAAGGTCAGAGGCCGCCTCTACCCCTGGGGTGTTGTGGAAGTGGAG AACCCAGAGCACAATGACTTTCTGAAGCTGAGAACCATGCTCATCACCCACATGCAGGATCTCCAGGAG GTGACCCAGGACCTTCATTATGAAAACTTCCGTTCTGAGAGACTCAAGAGAGGCGGCAGGAAAGTGGAG AATGAGGACATGAATAAAGACCAGATCTTGCTGGAAAAAGAAGCTGAGCTCCGCCGCATGCAAGAGATG ATTGCAAGGATGCAGGCGCAGATGCAGATGCAGATGCAGGGCGGGGATGGCGATGGCGGGGCTCTCGGG CACCACGTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282973
Insert Size	1116 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282973.1
RefSeq Size	3681 bp
RefSeq ORF	1116 bp
Locus ID	4735
UniProt ID	Q15019
MW	42.7 kDa
Gene Summary	Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830). Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) differs in the 5' UTR and contains an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (c) is longer than isoform a. Variants 6 and 15 encode the same isoform (c).

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