WWP2 (NM_001270454) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AIP2; WWp2-like
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332945 representing NM_001270454. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCATCTGCCAGCTCTAGCCGGGCAGGAGTGGCCCTGCCTTTTGAGAAGTCTCAGCTCACTTTGAAA GTGGTGTCCGCAAAGCCCAAGGTGCATAATCGTCAACCTCGAATTAACTCCTACGTGGAGGTGGCGGTG GATGGACTCCCCAGTGAGACCAAGAAGACTGGGAAGCGCATTGGGAGCTCTGAGCTTCTCTGGAATGAG ATCATCATTTTGAATGTCACGGCACAGAGTCATTTAGATTTAAAGGTCTGGAGCTGCCATACCTTGAGA AATGAACTGCTAGGCACCGCATCTGTCAACCTCTCCAACGTCTTGAAGAACAATGGGGGCAAAATGGAG AACATGCAGCTGACCCTGAACCTGCAGACGGAGAACAAAGGCAGCGTTGTCTCAGGCGGAGAGCTGACA ATTTTCCTGGACGGGCCAACTGTTGATCTGGGAAATGTGCCTAATGGCAGTGCCCTGACAGATGGATCA CAGCTGCCTTCGAGAGACTCCAGTGGAACAGCAGTAGCTCCAGAGAACCGGCACCAGCCCCCCAGCACA AACTGCTTTGGTGGAAGATCCCGGACGCACAGACATTCGGGTGCTTCAGCCAGAACAACCCCAGCAACC GGCGAGCAAAGCCCCGGTGCTCGGAGCCGGCACCGCCAGCCCGTCAAGAACTCAGGCCACAGTGGCTTG GCCAATGGCACAGTGAATGATGAACCCACAACAGCCACTGATCCCGAAGAACCTTCCGTTGTTGGTGTG ACGTCCCCACCTGCTGCACCCTTGAGTGTGACCCCGAATCCCAACACGACTTCTCTCCCTGCCCCAGCC ACACCGGCTGAAGGAGAGGAACCCAGCACTTCGGGTACACAGCAGCTCCCAGCGGCTGCCCAGGCCCCC GACGCTCTGCCTGCTGGATGGGAACAGCGAGAGCTGCCCAACGGACGTGTCTATTATGTTGACCACAAT ACCAAGACCACCACCTGGGAGCGGCCCCTTCCTCCAGGCTGGGAAAAACGCACAGATCCCCGAGGCAGG TTTTACTATGTGGATCACAATACTCGGACCACCACCTGGCAGCGTCCGACCGCGGAGTACGTGCGCAAC TATGAGCAGTGGCAGTCGCAGCGGAATCAGCTCCAGGGGGCCATGCAGCACTTCAGCCAAAGATTCCTC TACCAGTCTTCGAGTGCTTCGACTGACCATGATCCCCTGGGCCCCCTCCCTCCTGGCTGGGAGAAGAGA CAGGACAATGGACGGGTGTATTACGTGAACCATAACACTCGCACGACCCAGTGGGAGGATCCCCGGACC CAGGGGATGATCCAGGAACCAGCTCTGCCCCCAGGATGGGAGATGAAATACACCAGCGAGGGGGTGCGA TACTTTGTGGACCACAATACCCGCACCACCACCTTTAAGGATCCTCGCCCGGGGTTTGAGTCGGGGACG AAGCAAGGTTCCCCTGGTGCTTATGACCGCAGTTTTCGGTGGAAGTATCACCAGTTCCGTTTCCTCTGC CATTCAAATGCCCTACCTAGCCACGTGAAGATCAGCGTTTCCAGGCAGACGCTTTTCGAAGATTCCTTC CAACAGATCATGAACATGAAACCCTATGACCTGCGCCGCCGGCTCTACATCATCATGCGTGGCGAGGAG GGCCTGGACTATGGGGGCATCGCCAGAGAGTGGTTTTTCCTCCTGTCTCATGAGGTGCTCAACCCTATG TATTGTTTATTTGAATATGCCGGAAAGAACAATTACTGCCTGCAGATCAACCCCGCCTCCTCCATCAAC CCGGACCACCTCACCTACTTTCGCTTTATAGGCAGATTCATCGCCATGGCGCTGTACCATGGAAAGTTC ATCGACACGGGCTTCACCCTCCCTTTCTACAAGCGGATGCTCAATAAGAGACCAACCCTGAAAGACCTG GAGTCCATTGACCCTGAGTTCTACAACTCCATTGTCTGGATCAAAGAGAACAACCTGGAAGAATGTGGC CTGGAGCTGTACTTCATCCAGGACATGGAGATACTGGGCAAGGTGACGACCCACGAGCTGAAGGAGGGC GGCGAGAGCATCCGGGTCACAGAGGAGAACAAGGAAGAGTACATCATGCTGCTGACTGACTGGCGTTTC ACCCGAGGCGTGGAAGAGCAGACCAAAGCCTTCCTGGATGGCTTCAACGAGGTGGCCCCGCTGGAGTGG CTGCGCTACTTTGACGAGAAAGAGCTGGAGCTGATGCTGTGCGGCATGCAGGAGATAGACATGAGCGAC TGGCAGAAGAGCACCATCTACCGGCACTACACCAAGAACAGCAAGCAGATCCAGTGGTTCTGGCAGGTG GTGAAGGAGATGGACAACGAGAAGAGGATCCGGCTGCTGCAGTTTGTCACCGGTACCTGCCGCCTGCCC GTCGGGGGATTTGCCGAACTCATCGGTAGCAACGGACCACAGAAGTTTTGCATTGACAAAGTTGGCAAG GAAACCTGGCTGCCCAGAAGCCACACCTGCTTCAACCGTCTGGATCTTCCACCCTACAAGAGCTACGAA CAGCTGAGAGAGAAGCTGCTGTATGCCATTGAGGAGACCGAGGGCTTTGGACAGGAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270454
Insert Size	2613 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270454.1
RefSeq Size	4555 bp
RefSeq ORF	2613 bp
Locus ID	11060
UniProt ID	O00308
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
MW	98.9 kDa
Gene Summary	This gene encodes a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. The encoded protein contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 10. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (5) differs in the 5' UTR, compared to variant 1. Variants 1 and 5 encode the same isoform (WWP2-FL).

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