WWP2 (NM_001270453) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AIP2; WWp2-like
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332944 representing NM_001270453. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCAGCTGACCCTGAACCTGCAGACGGAGAACAAAGGCAGCGTTGTCTCAGGCGGAGAGCTGACAATT TTCCTGGACGGGCCAACTGTTGATCTGGGAAATGTGCCTAATGGCAGTGCCCTGACAGATGGATCACAG CTGCCTTCGAGAGACTCCAGTGGAACAGCAGTAGCTCCAGAGAACCGGCACCAGCCCCCCAGCACAAAC TGCTTTGGTGGAAGATCCCGGACGCACAGACATTCGGGTGCTTCAGCCAGAACAACCCCAGCAACCGGC GAGCAAAGCCCCGGTGCTCGGAGCCGGCACCGCCAGCCCGTCAAGAACTCAGGCCACAGTGGCTTGGCC AATGGCACAGTGAATGATGAACCCACAACAGCCACTGATCCCGAAGAACCTTCCGTTGTTGGTGTGACG TCCCCACCTGCTGCACCCTTGAGTGTGACCCCGAATCCCAACACGACTTCTCTCCCTGCCCCAGCCACA CCGGCTGAAGGAGAGGAACCCAGCACTTCGGGTACACAGCAGCTCCCAGCGGCTGCCCAGGCCCCCGAC GCTCTGCCTGCTGGATGGGAACAGCGAGAGCTGCCCAACGGACGTGTCTATTATGTTGACCACAATACC AAGACCACCACCTGGGAGCGGCCCCTTCCTCCAGGCTGGGAAAAACGCACAGATCCCCGAGGCAGGTTT TACTATGTGGATCACAATACTCGGACCACCACCTGGCAGCGTCCGACCGCGGAGTACGTGCGCAACTAT GAGCAGTGGCAGTCGCAGCGGAATCAGCTCCAGGGGGCCATGCAGCACTTCAGCCAAAGATTCCTCTAC CAGTCTTCGAGTGCTTCGACTGACCATGATCCCCTGGGCCCCCTCCCTCCTGGCTGGGAGAAGAGACAG GACAATGGACGGGTGTATTACGTGAACCATAACACTCGCACGACCCAGTGGGAGGATCCCCGGACCCAG GGGATGATCCAGGAACCAGCTCTGCCCCCAGGATGGGAGATGAAATACACCAGCGAGGGGGTGCGATAC TTTGTGGACCACAATACCCGCACCACCACCTTTAAGGATCCTCGCCCGGGGTTTGAGTCGGGGACGAAG CAAGGTTCCCCTGGTGCTTATGACCGCAGTTTTCGGTGGAAGTATCACCAGTTCCGTTTCCTCTGCCAT TCAAATGCCCTACCTAGCCACGTGAAGATCAGCGTTTCCAGGCAGACGCTTTTCGAAGATTCCTTCCAA CAGATCATGAACATGAAACCCTATGACCTGCGCCGCCGGCTCTACATCATCATGCGTGGCGAGGAGGGC CTGGACTATGGGGGCATCGCCAGAGAGTGGTTTTTCCTCCTGTCTCATGAGGTGCTCAACCCTATGTAT TGTTTATTTGAATATGCCGGAAAGAACAATTACTGCCTGCAGATCAACCCCGCCTCCTCCATCAACCCG GACCACCTCACCTACTTTCGCTTTATAGGCAGATTCATCGCCATGGCGCTGTACCATGGAAAGTTCATC GACACGGGCTTCACCCTCCCTTTCTACAAGCGGATGCTCAATAAGAGACCAACCCTGAAAGACCTGGAG TCCATTGACCCTGAGTTCTACAACTCCATTGTCTGGATCAAAGAGAACAACCTGGAAGAATGTGGCCTG GAGCTGTACTTCATCCAGGACATGGAGATACTGGGCAAGGTGACGACCCACGAGCTGAAGGAGGGCGGC GAGAGCATCCGGGTCACAGAGGAGAACAAGGAAGAGTACATCATGCTGCTGACTGACTGGCGTTTCACC CGAGGCGTGGAAGAGCAGACCAAAGCCTTCCTGGATGGCTTCAACGAGGTGGCCCCGCTGGAGTGGCTG CGCTACTTTGACGAGAAAGAGCTGGAGCTGATGCTGTGCGGCATGCAGGAGATAGACATGAGCGACTGG CAGAAGAGCACCATCTACCGGCACTACACCAAGAACAGCAAGCAGATCCAGTGGTTCTGGCAGGTGGTG AAGGAGATGGACAACGAGAAGAGGATCCGGCTGCTGCAGTTTGTCACCGGTACCTGCCGCCTGCCCGTC GGGGGATTTGCCGAACTCATCGGTAGCAACGGACCACAGAAGTTTTGCATTGACAAAGTTGGCAAGGAA ACCTGGCTGCCCAGAAGCCACACCTGCTTCAACCGTCTGGATCTTCCACCCTACAAGAGCTACGAACAG CTGAGAGAGAAGCTGCTGTATGCCATTGAGGAGACCGAGGGCTTTGGACAGGAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270453
Insert Size	2265 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270453.1
RefSeq Size	4245 bp
RefSeq ORF	2265 bp
Locus ID	11060
UniProt ID	O00308
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
MW	86.3 kDa
Gene Summary	This gene encodes a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. The encoded protein contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 10. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (4) has a shorter N-terminus, compared to isoform 1.

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