TRPM1 (NM_001252020) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CSNB1C; LTRPC1; MLSN1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332164 representing NM_001252020. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGCTCCTTCAAGCGGGGCTCGCTCAAGAGCTCCACATCAGGGTCGCAGAAGGGTCAGAAATCTTGG ATAGAGAAAACCTTTTGCAAACGGGAATGTATCTTTGTAATTCCTAGCATGAAAGACTCTAACAGGTGT TGCTGTGGCCAGTTCACCAACCAGCATATCCCCCCTCTGCCAAGTGCAACACCCAGCAAAAATGAAGAG GAAAGCAAACAGGTGGAGACTCAGCCTGAGAAATGGTCTGTTGCCAAGCACACCCAGAGCTACCCAACA GATTCCTATGGAGTTCTTGAATTCCAGGGTGGCGGATATTCCAATAAAGCCATGTATATCCGTGTATCC TATGACACCAAGCCAGACTCACTGCTCCATCTCATGGTGAAAGATTGGCAGCTGGAACTCCCCAAGCTC TTAATATCTGTGCATGGAGGCCTCCAGAACTTTGAGATGCAGCCCAAGCTGAAACAAGTCTTTGGGAAA GGCCTGATCAAGGCTGCTATGACCACCGGGGCCTGGATCTTCACCGGGGGTGTCAGCACAGGTGTTATC AGCCACGTAGGGGATGCCTTGAAAGACCACTCCTCCAAGTCCAGAGGCCGGGTTTGTGCTATAGGAATT GCTCCATGGGGCATCGTGGAGAATAAGGAAGACCTGGTTGGAAAGGATGTAACAAGAGTGTACCAGACC ATGTCCAACCCTCTAAGTAAGCTCTCTGTGCTCAACAACTCCCACACCCACTTCATCCTGGCTGACAAT GGCACCCTGGGCAAGTATGGCGCCGAGGTGAAGCTGCGAAGGCTGCTGGAAAAGCACATCTCCCTGCAG AAGATCAACACAAGACTGGGGCAGGGCGTGCCCCTCGTGGGTCTCGTGGTGGAGGGGGGCCCTAACGTG GTGTCCATCGTCTTGGAATACCTGCAAGAAGAGCCTCCCATCCCTGTGGTGATTTGTGATGGCAGCGGA CGTGCCTCGGACATCCTGTCCTTTGCGCACAAGTACTGTGAAGAAGGCGGAATAATAAATGAGTCCCTC AGGGAGCAGCTTCTAGTTACCATTCAGAAAACATTTAATTATAATAAGGCACAATCACATCAGCTGTTT GCAATTATAATGGAGTGCATGAAGAAGAAAGAACTCGTCACTGTGTTCAGAATGGGTTCTGAGGGCCAG CAGGACATCGAGATGGCAATTTTAACTGCCCTGCTGAAAGGAACAAACGTATCTGCTCCAGATCAGCTG AGCTTGGCACTGGCTTGGAACCGCGTGGACATAGCACGAAGCCAGATCTTTGTCTTTGGGCCCCACTGG CCGCCCCTGGGAAGCCTGGCACCCCCGACGGACAGCAAAGCCACGGAGAAGGAGAAGAAGCCACCCATG GCCACCACCAAGGGAGGAAGAGGAAAAGGGAAAGGCAAGAAGAAAGGGAAAGTGAAAGAGGAAGTGGAG GAAGAAACTGACCCCCGGAAGATAGAGCTGCTGAACTGGGTGAATGCTTTGGAGCAAGCGATGCTAGAT GCTTTAGTCTTAGATCGTGTCGACTTTGTGAAGCTCCTGATTGAAAACGGAGTGAACATGCAACACTTT CTGACCATTCCGAGGCTGGAGGAGCTTTATAACACAAGACTGGGTCCACCAAACACACTTCATCTGCTG GTGAGGGATGTGAAAAAGAGCAACCTTCCGCCTGATTACCACATCAGCCTCATAGACATCGGGCTCGTG CTGGAGTACCTCATGGGAGGAGCCTACCGCTGCAACTACACTCGGAAAAACTTTCGGACCCTTTACAAC AACTTGTTTGGACCAAAGAGGCCTAAAGCTCTTAAACTTCTGGGAATGGAAGATGATGAGCCTCCAGCT AAAGGGAAGAAAAAGAAAAAGAAGAAAAAGGAGGAAGAGATCGACATTGATGTGGACGACCCTGCCGTG AGTCGGTTCCAGTATCCCTTCCACGAGCTGATGGTGTGGGCAGTGCTGATGAAACGCCAGAAAATGGCA GTGTTCCTCTGGCAGCGAGGGGAAGAGAGCATGGCCAAGGCCCTGGTGGCCTGCAAGCTCTACAAGGCC ATGGCCCACGAGTCCTCCGAGAGTGATCTGGTGGATGACATCTCCCAGGACTTGGATAACAATTCCAAA GACTTCGGCCAGCTTGCTTTGGAGTTATTAGACCAGTCCTATAAGCATGACGAGCAGATCGCTATGAAA CTCCTGACCTACGAGCTGAAAAACTGGAGCAACTCGACCTGCCTCAAACTGGCCGTGGCAGCCAAACAC CGGGACTTCATTGCTCACACCTGCAGCCAGATGCTGCTGACCGATATGTGGATGGGAAGACTGCGGATG CGGAAGAACCCCGGCCTGAAGGTTATCATGGGGATTCTTCTACCCCCCACCATCTTGTTTTTGGAATTT CGCACATATGATGATTTCTCGTATCAAACATCCAAGGAAAATGAGGATGGCAAAGAAAAAGAAGAGGAA AATACGGATGCAAATGCAGATGCTGGCTCAAGAAAGGGGGATGAGGAGAACGAGCACAAAAAACAGAGA AGTATTCCCATCGGAACAAAGATCTGTGAATTCTATAACGCGCCCATTGTCAAGTTCTGGTTTTACACA ATATCATACTTGGGCTACCTGCTGCTGTTTAACTACGTCATCCTGGTGCGGATGGATGGCTGGCCGTCC CTCCAGGAGTGGATCGTCATCTCCTACATCGTGAGCCTGGCGTTAGAGAAGATACGAGAGATCCTCATG TCAGAACCAGGCAAACTCAGCCAGAAAATCAAAGTTTGGCTTCAGGAGTACTGGAACATCACAGATCTC GTGGCCATTTCCACATTCATGATTGGAGCAATTCTTCGCCTACAGAACCAGCCCTACATGGGCTATGGC CGGGTGATCTACTGTGTGGATATCATCTTCTGGTACATCCGTGTCCTGGACATCTTTGGTGTCAACAAG TATCTGGGGCCATACGTGATGATGATTGGAAAGATGATGATCGACATGCTGTACTTTGTGGTCATCATG CTGGTCGTGCTCATGAGTTTCGGAGTAGCCCGTCAAGCCATTCTGCATCCAGAGGAGAAGCCCTCTTGG AAACTGGCCCGAAACATCTTCTACATGCCCTACTGGATGATCTATGGAGAGGTGTTTGCAGACCAGATA GACCTCTACGCCATGGAAATTAATCCTCCTTGTGGTGAGAACCTATATGATGAGGAGGGCAAGCGGCTT CCTCCCTGTATCCCCGGCGCCTGGCTCACTCCAGCACTCATGGCGTGCTATCTACTGGTCGCCAACATC CTGCTGGTGAACCTGCTGATTGCTGTGTTCAACAATACCTTCTTTGAAGTAAAATCAATATCCAACCAG GTGTGGAAGTTCCAGCGATATCAGCTGATTATGACATTTCATGACAGGCCAGTCCTGCCCCCACCGATG ATCATTTTAAGCCACATCTACATCATCATTATGCGTCTCAGCGGCCGCTGCAGGAAAAAGAGAGAAGGG GACCAAGAGGAACGGGATCGTGGATTGAAGCTCTTCCTTAGCGACGAGGAGCTAAAGAGGCTGCATGAG TTCGAGGAGCAGTGCGTGCAGGAGCACTTCCGGGAGAAGGAGGATGAGCAGCAGTCGTCCAGCGACGAG CGCATCCGGGTCACTTCTGAAAGAGTTGAAAATATGTCAATGAGGTTGGAAGAAATCAATGAAAGAGAA ACTTTTATGAAAACTTCCCTGCAGACTGTTGACCTTCGACTTGCTCAGCTAGAAGAATTATCTAACAGA ATGGTGAATGCTCTTGAAAATCTTGCGGGAATCGACAGGTCTGACCTGATCCAGGCACGGTCCCGGGCT TCTTCTGAATGTGAGGCAACGTATCTTCTCCGGCAAAGCAGCATCAATAGCGCTGATGGCTACAGCTTG TATCGATATCATTTTAACGGAGAAGAGTTATTATTTGAGGATACATCTCTCTCCACGTCACCAGGGACA GGAGTCAGGAAAAAAACCTGTTCCTTCCGTATAAAGGAAGAGAAGGACGTGAAAACGCACCTAGTCCCA GAATGTCAGAACAGTCTTCACCTTTCACTGGGCACAAGCACATCAGCAACCCCAGATGGCAGTCACCTT GCAGTAGATGACTTAAAGAACGCTGAAGAGTCAAAATTAGGTCCAGATATTGGGATTTCAAAGGAAGAT GATGAAAGACAGACAGACTCTAAAAAAGAAGAAACTATTTCCCCAAGTTTAAATAAAACAGATGTGATA CATGGACAGGACAAATCAGATGTTCAAAACACTCAGCTAACAGTGGAAACGACAAATATAGAAGGCACT ATTTCCTATCCCCTGGAAGAAACCAAAATTACACGCTATTTCCCCGATGAAACGATCAATGCTTGTAAA ACAATGAAGTCCAGAAGCTTCGTCTATTCCCGGGGAAGAAAGCTGGTCGGTGGGGTTAACCAGGATGTA GAGTACAGTTCAATCACGGACCAGCAATTGACGACGGAATGGCAATGCCAAGTTCAAAAGATCACGCGC TCTCATAGCACAGATATTCCTTACATTGTGTCGGAAGCTGCAGTGCAAGCTGAGCATAAAGAGCAGTTT GCAGATATGCAAGATGAACACCATGTCGCTGAAGCAATTCCTCGAATCCCTCGCTTGTCCCTAACCATT ACTGACAGAAATGGGATGGAAAACTTACTGTCTGTGAAGCCAGATCAAACTTTGGGATTCCCATCTCTC AGGTCAAAAAGTTTACATGGACATCCTAGGAATGTGAAATCCATTCAGGGAAAGTTAGACAGATCTGGA CATGCCAGTAGTGTAAGCAGCTTAGTAATTGTGTCTGGAATGACAGCAGAAGAAAAAAAGGTTAAGAAA GAGAAAGCTTCCACAGAAACTGAATGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001252020
Insert Size	4929 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252020.1
RefSeq Size	6022 bp
RefSeq ORF	4929 bp
Locus ID	4308
UniProt ID	Q7Z4N2
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
MW	186.5 kDa
Gene Summary	This gene encodes a member of the transient receptor potential melastatin subfamily of transient receptor potential ion channels. The encoded protein is a calcium permeable cation channel that is expressed in melanocytes and may play a role in melanin synthesis. Specific mutations in this gene are the cause autosomal recessive complete congenital stationary night blindness-1C. The expression of this protein is inversely correlated with melanoma aggressiveness and as such it is used as a prognostic marker for melanoma metastasis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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