TRPM1 (NM_001252020) Human Tagged ORF Clone
CAT#: RG235326
- TrueORF®
TRPM1 (tGFP-tagged) - Homo sapiens transient receptor potential cation channel, subfamily M, member 1 (TRPM1), transcript variant 1
ORF Plasmid: DDK
"NM_001252020" in other vectors (2)
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CNY 17,960.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CSNB1C; LTRPC1; MLSN1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG235326 representing NM_001252020
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCCTTCAAGCGGGGCTCGCTCAAGAGCTCCACATCAGGGTCGCAGAAGGGTCAGAAATCTTGGA TAGAGAAAACCTTTTGCAAACGGGAATGTATCTTTGTAATTCCTAGCATGAAAGACTCTAACAGGTGTTG CTGTGGCCAGTTCACCAACCAGCATATCCCCCCTCTGCCAAGTGCAACACCCAGCAAAAATGAAGAGGAA AGCAAACAGGTGGAGACTCAGCCTGAGAAATGGTCTGTTGCCAAGCACACCCAGAGCTACCCAACAGATT CCTATGGAGTTCTTGAATTCCAGGGTGGCGGATATTCCAATAAAGCCATGTATATCCGTGTATCCTATGA CACCAAGCCAGACTCACTGCTCCATCTCATGGTGAAAGATTGGCAGCTGGAACTCCCCAAGCTCTTAATA TCTGTGCATGGAGGCCTCCAGAACTTTGAGATGCAGCCCAAGCTGAAACAAGTCTTTGGGAAAGGCCTGA TCAAGGCTGCTATGACCACCGGGGCCTGGATCTTCACCGGGGGTGTCAGCACAGGTGTTATCAGCCACGT AGGGGATGCCTTGAAAGACCACTCCTCCAAGTCCAGAGGCCGGGTTTGTGCTATAGGAATTGCTCCATGG GGCATCGTGGAGAATAAGGAAGACCTGGTTGGAAAGGATGTAACAAGAGTGTACCAGACCATGTCCAACC CTCTAAGTAAGCTCTCTGTGCTCAACAACTCCCACACCCACTTCATCCTGGCTGACAATGGCACCCTGGG CAAGTATGGCGCCGAGGTGAAGCTGCGAAGGCTGCTGGAAAAGCACATCTCCCTGCAGAAGATCAACACA AGACTGGGGCAGGGCGTGCCCCTCGTGGGTCTCGTGGTGGAGGGGGGCCCTAACGTGGTGTCCATCGTCT TGGAATACCTGCAAGAAGAGCCTCCCATCCCTGTGGTGATTTGTGATGGCAGCGGACGTGCCTCGGACAT CCTGTCCTTTGCGCACAAGTACTGTGAAGAAGGCGGAATAATAAATGAGTCCCTCAGGGAGCAGCTTCTA GTTACCATTCAGAAAACATTTAATTATAATAAGGCACAATCACATCAGCTGTTTGCAATTATAATGGAGT GCATGAAGAAGAAAGAACTCGTCACTGTGTTCAGAATGGGTTCTGAGGGCCAGCAGGACATCGAGATGGC AATTTTAACTGCCCTGCTGAAAGGAACAAACGTATCTGCTCCAGATCAGCTGAGCTTGGCACTGGCTTGG AACCGCGTGGACATAGCACGAAGCCAGATCTTTGTCTTTGGGCCCCACTGGCCGCCCCTGGGAAGCCTGG CACCCCCGACGGACAGCAAAGCCACGGAGAAGGAGAAGAAGCCACCCATGGCCACCACCAAGGGAGGAAG AGGAAAAGGGAAAGGCAAGAAGAAAGGGAAAGTGAAAGAGGAAGTGGAGGAAGAAACTGACCCCCGGAAG ATAGAGCTGCTGAACTGGGTGAATGCTTTGGAGCAAGCGATGCTAGATGCTTTAGTCTTAGATCGTGTCG ACTTTGTGAAGCTCCTGATTGAAAACGGAGTGAACATGCAACACTTTCTGACCATTCCGAGGCTGGAGGA GCTTTATAACACAAGACTGGGTCCACCAAACACACTTCATCTGCTGGTGAGGGATGTGAAAAAGAGCAAC CTTCCGCCTGATTACCACATCAGCCTCATAGACATCGGGCTCGTGCTGGAGTACCTCATGGGAGGAGCCT ACCGCTGCAACTACACTCGGAAAAACTTTCGGACCCTTTACAACAACTTGTTTGGACCAAAGAGGCCTAA AGCTCTTAAACTTCTGGGAATGGAAGATGATGAGCCTCCAGCTAAAGGGAAGAAAAAGAAAAAGAAGAAA AAGGAGGAAGAGATCGACATTGATGTGGACGACCCTGCCGTGAGTCGGTTCCAGTATCCCTTCCACGAGC TGATGGTGTGGGCAGTGCTGATGAAACGCCAGAAAATGGCAGTGTTCCTCTGGCAGCGAGGGGAAGAGAG CATGGCCAAGGCCCTGGTGGCCTGCAAGCTCTACAAGGCCATGGCCCACGAGTCCTCCGAGAGTGATCTG GTGGATGACATCTCCCAGGACTTGGATAACAATTCCAAAGACTTCGGCCAGCTTGCTTTGGAGTTATTAG ACCAGTCCTATAAGCATGACGAGCAGATCGCTATGAAACTCCTGACCTACGAGCTGAAAAACTGGAGCAA CTCGACCTGCCTCAAACTGGCCGTGGCAGCCAAACACCGGGACTTCATTGCTCACACCTGCAGCCAGATG CTGCTGACCGATATGTGGATGGGAAGACTGCGGATGCGGAAGAACCCCGGCCTGAAGGTTATCATGGGGA TTCTTCTACCCCCCACCATCTTGTTTTTGGAATTTCGCACATATGATGATTTCTCGTATCAAACATCCAA GGAAAATGAGGATGGCAAAGAAAAAGAAGAGGAAAATACGGATGCAAATGCAGATGCTGGCTCAAGAAAG GGGGATGAGGAGAACGAGCACAAAAAACAGAGAAGTATTCCCATCGGAACAAAGATCTGTGAATTCTATA ACGCGCCCATTGTCAAGTTCTGGTTTTACACAATATCATACTTGGGCTACCTGCTGCTGTTTAACTACGT CATCCTGGTGCGGATGGATGGCTGGCCGTCCCTCCAGGAGTGGATCGTCATCTCCTACATCGTGAGCCTG GCGTTAGAGAAGATACGAGAGATCCTCATGTCAGAACCAGGCAAACTCAGCCAGAAAATCAAAGTTTGGC TTCAGGAGTACTGGAACATCACAGATCTCGTGGCCATTTCCACATTCATGATTGGAGCAATTCTTCGCCT ACAGAACCAGCCCTACATGGGCTATGGCCGGGTGATCTACTGTGTGGATATCATCTTCTGGTACATCCGT GTCCTGGACATCTTTGGTGTCAACAAGTATCTGGGGCCATACGTGATGATGATTGGAAAGATGATGATCG ACATGCTGTACTTTGTGGTCATCATGCTGGTCGTGCTCATGAGTTTCGGAGTAGCCCGTCAAGCCATTCT GCATCCAGAGGAGAAGCCCTCTTGGAAACTGGCCCGAAACATCTTCTACATGCCCTACTGGATGATCTAT GGAGAGGTGTTTGCAGACCAGATAGACCTCTACGCCATGGAAATTAATCCTCCTTGTGGTGAGAACCTAT ATGATGAGGAGGGCAAGCGGCTTCCTCCCTGTATCCCCGGCGCCTGGCTCACTCCAGCACTCATGGCGTG CTATCTACTGGTCGCCAACATCCTGCTGGTGAACCTGCTGATTGCTGTGTTCAACAATACCTTCTTTGAA GTAAAATCAATATCCAACCAGGTGTGGAAGTTCCAGCGATATCAGCTGATTATGACATTTCATGACAGGC CAGTCCTGCCCCCACCGATGATCATTTTAAGCCACATCTACATCATCATTATGCGTCTCAGCGGCCGCTG CAGGAAAAAGAGAGAAGGGGACCAAGAGGAACGGGATCGTGGATTGAAGCTCTTCCTTAGCGACGAGGAG CTAAAGAGGCTGCATGAGTTCGAGGAGCAGTGCGTGCAGGAGCACTTCCGGGAGAAGGAGGATGAGCAGC AGTCGTCCAGCGACGAGCGCATCCGGGTCACTTCTGAAAGAGTTGAAAATATGTCAATGAGGTTGGAAGA AATCAATGAAAGAGAAACTTTTATGAAAACTTCCCTGCAGACTGTTGACCTTCGACTTGCTCAGCTAGAA GAATTATCTAACAGAATGGTGAATGCTCTTGAAAATCTTGCGGGAATCGACAGGTCTGACCTGATCCAGG CACGGTCCCGGGCTTCTTCTGAATGTGAGGCAACGTATCTTCTCCGGCAAAGCAGCATCAATAGCGCTGA TGGCTACAGCTTGTATCGATATCATTTTAACGGAGAAGAGTTATTATTTGAGGATACATCTCTCTCCACG TCACCAGGGACAGGAGTCAGGAAAAAAACCTGTTCCTTCCGTATAAAGGAAGAGAAGGACGTGAAAACGC ACCTAGTCCCAGAATGTCAGAACAGTCTTCACCTTTCACTGGGCACAAGCACATCAGCAACCCCAGATGG CAGTCACCTTGCAGTAGATGACTTAAAGAACGCTGAAGAGTCAAAATTAGGTCCAGATATTGGGATTTCA AAGGAAGATGATGAAAGACAGACAGACTCTAAAAAAGAAGAAACTATTTCCCCAAGTTTAAATAAAACAG ATGTGATACATGGACAGGACAAATCAGATGTTCAAAACACTCAGCTAACAGTGGAAACGACAAATATAGA AGGCACTATTTCCTATCCCCTGGAAGAAACCAAAATTACACGCTATTTCCCCGATGAAACGATCAATGCT TGTAAAACAATGAAGTCCAGAAGCTTCGTCTATTCCCGGGGAAGAAAGCTGGTCGGTGGGGTTAACCAGG ATGTAGAGTACAGTTCAATCACGGACCAGCAATTGACGACGGAATGGCAATGCCAAGTTCAAAAGATCAC GCGCTCTCATAGCACAGATATTCCTTACATTGTGTCGGAAGCTGCAGTGCAAGCTGAGCATAAAGAGCAG TTTGCAGATATGCAAGATGAACACCATGTCGCTGAAGCAATTCCTCGAATCCCTCGCTTGTCCCTAACCA TTACTGACAGAAATGGGATGGAAAACTTACTGTCTGTGAAGCCAGATCAAACTTTGGGATTCCCATCTCT CAGGTCAAAAAGTTTACATGGACATCCTAGGAATGTGAAATCCATTCAGGGAAAGTTAGACAGATCTGGA CATGCCAGTAGTGTAAGCAGCTTAGTAATTGTGTCTGGAATGACAGCAGAAGAAAAAAAGGTTAAGAAAG AGAAAGCTTCCACAGAAACTGAATGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235326 representing NM_001252020
Red=Cloning site Green=Tags(s) MSSFKRGSLKSSTSGSQKGQKSWIEKTFCKRECIFVIPSMKDSNRCCCGQFTNQHIPPLPSATPSKNEEE SKQVETQPEKWSVAKHTQSYPTDSYGVLEFQGGGYSNKAMYIRVSYDTKPDSLLHLMVKDWQLELPKLLI SVHGGLQNFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGVSTGVISHVGDALKDHSSKSRGRVCAIGIAPW GIVENKEDLVGKDVTRVYQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRLLEKHISLQKINT RLGQGVPLVGLVVEGGPNVVSIVLEYLQEEPPIPVVICDGSGRASDILSFAHKYCEEGGIINESLREQLL VTIQKTFNYNKAQSHQLFAIIMECMKKKELVTVFRMGSEGQQDIEMAILTALLKGTNVSAPDQLSLALAW NRVDIARSQIFVFGPHWPPLGSLAPPTDSKATEKEKKPPMATTKGGRGKGKGKKKGKVKEEVEEETDPRK IELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVRDVKKSN LPPDYHISLIDIGLVLEYLMGGAYRCNYTRKNFRTLYNNLFGPKRPKALKLLGMEDDEPPAKGKKKKKKK KEEEIDIDVDDPAVSRFQYPFHELMVWAVLMKRQKMAVFLWQRGEESMAKALVACKLYKAMAHESSESDL VDDISQDLDNNSKDFGQLALELLDQSYKHDEQIAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQM LLTDMWMGRLRMRKNPGLKVIMGILLPPTILFLEFRTYDDFSYQTSKENEDGKEKEEENTDANADAGSRK GDEENEHKKQRSIPIGTKICEFYNAPIVKFWFYTISYLGYLLLFNYVILVRMDGWPSLQEWIVISYIVSL ALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISTFMIGAILRLQNQPYMGYGRVIYCVDIIFWYIR VLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIY GEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFE VKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDRGLKLFLSDEE LKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERETFMKTSLQTVDLRLAQLE ELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLST SPGTGVRKKTCSFRIKEEKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGIS KEDDERQTDSKKEETISPSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINA CKTMKSRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEHKEQ FADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLHGHPRNVKSIQGKLDRSG HASSVSSLVIVSGMTAEEKKVKKEKASTETEC TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001252020 |
ORF Size | 4926 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001252020.1, NP_001238949.1 |
RefSeq Size | 6022 bp |
RefSeq ORF | 4929 bp |
Locus ID | 4308 |
UniProt ID | Q7Z4N2 |
Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
Gene Summary | This gene encodes a member of the transient receptor potential melastatin subfamily of transient receptor potential ion channels. The encoded protein is a calcium permeable cation channel that is expressed in melanocytes and may play a role in melanin synthesis. Specific mutations in this gene are the cause autosomal recessive complete congenital stationary night blindness-1C. The expression of this protein is inversely correlated with melanoma aggressiveness and as such it is used as a prognostic marker for melanoma metastasis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC235326 | TRPM1 (Myc-DDK tagged) - Homo sapiens transient receptor potential cation channel, subfamily M, member 1 (TRPM1), transcript variant 1 |
CNY 16,340.00 |
|
SC332164 | TRPM1 (untagged) - Homo sapiens transient receptor potential cation channel, subfamily M, member 1 (TRPM1), transcript variant 1 |
CNY 15,770.00 |