KIAA0586 (NM_001244190) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	JBTS23; SRTD14; Talpid3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332067 representing NM_001244190. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCAGTGAAGAGACTTCGTGAGGTAGTTTCTCAAAATCATGGAGATCATTTGGTTTTGCTGAAAGAT GAGTTGCCCTGTGTTCCTCCGGCATTGTCTGCAAATAAACGTCTTCCTGTTGGAACGGGGACTAGTTTG AATGGAACATCACGTGGTTCATCAGACTTAACTTCTGCTAGAAATTGTTACCAGCCTCTATTAGAAAAT CCCATGGTGTCAGAAAGTGATTTTTCTAAAGACGTTGCAGTGCAAGTGTTGCCTTTGGATAAAATAGAA GAGAACAACAAGCAAAAAGCAAATGACATCTTCATTTCTCAGTATACAATGGGACAGAAAGATGCTCTA AGAACAGTTTTAAAGCAAAAAGCTCAAAGCATGCCTGTTTTTAAGGAAGTAAAGGTACATCTGTTAGAA GATGCAGGCATAGAGAAGGATGCTGTTACTCAGGAGACTAGAATTTCACCCAGTGGAATTGATTCAGCT ACAACCGTGGCTGCAGCAACTGCTGCTGCCATTGCAACCGCAGCTCCGTTGATAAAGGTGCAGAGTGAT TTGGAAGCAAAAGTCAATTCTGTTACAGAATTACTTAGTAAATTACAGGAGACTGATAAACACCTGCAA CGTGTTACAGAGCAGCAAACAAGCATTCAGAGGAAACAAGAGAAATTACATTGTCATGATCACGAAAAG CAAATGAATGTGTTTATGGAGCAGCACATAAGGCATCTTGAAAAGTTACAACAACAACAAATAGATATT CAGACTCATTTTATTAGTGCTGCACTCAAGACTAGTAGTTTTCAGCCTGTTAGTATGCCCTCCTCCAGA GCAGTGGAAAAGTATTCCGTAAAACCAGAACACCCTAATCTTGGTAGCTGTAATCCATCTTTATATAAC ACATTTGCTTCCAAACAAGCACCTTTAAAAGAAGTTGAAGATACGAGTTTTGATAAACAGAAATCTCCT TTGGAGACACCAGCACCTCGCAGATTTGCTCCTGTACCTGTTTCAAGGGATGATGAACTATCAAAGAGG GAAAATCTTTTGGAAGAAAAAGAAAATATGGAAGTGTCGTGTCACAGAGGAAATGTAAGACTATTGGAA CAAATTTTGAATAATAATGATTCTTTGACAAGAAAAAGTGAATCATCAAACACCACCTCACTAACTAGG TCAAAAATAGGATGGACTCCTGAGAAAACAAACAGATTTCCTTCCTGTGAAGAGCTAGAAACAACTAAA GTGACTATGCAGAAGTCTGATGATGTTCTTCATGACCTTGGCCAAAAAGAGAAAGAAACAAATAGCATG GTCCAGCCAAAAGAATCTCTGAGTATGTTGAAGCTTCCAGATCTTCCACAGAATTCTGTTAAGCTTCAA ACAACCAATACAACAAGATCTGTATTGAAAGATGCTGAGAAGATTTTGAGAGGAGTACAAAACAATAAA AAAGTACTTGAAGAAAACCTGGAAGCTATTATTCGTGCAAAAGATGGAGCTGCCATGTATTCGCTTATC AATGCTTTATCTACCAACAGAGAGATGTCAGAGAAAATTAGGATCAGAAAGACAGTGGATGAATGGATT AAAACTATTTCTGCAGAAATTCAGGATGAACTGTCAAGAACAGATTATGAACAAAAAAGATTTGATCAG AAGAATCAGAGAACCAAGAAAGGTCAGAATATGACTAAAGATATTAGAACCAACACACAAGATAAAACT GTCAACAAATCTGTAATTCCAAGAAAACATTCTCAAAAGCAAATAGAAGAGCATTTTAGAAATCTACCT ATGAGGGGCATGCCTGCTTCAAGTTTACAGAAAGAGAGAAAGGAAGGGCTTTTGAAAGCAACCACAGTA ATACAAGATGAAGATTATATGTTACAAGTCTATGGAAAGCCAGTTTATCAGGGCCATCGAAGCACTCTT AAAAAAGGACCATATCTCAGATTTAATTCTCCATCTCCTAAGTCCAGACCACAGAGACCAAAAGTAATA GAACGAGTTAAAGGCACTAAGGTAAAGTCAATAAGAACACAGACTGACTTCTATGCAACAAAACCTAAG AAGATGGATTCTAAAATGAAACATTCTGTTCCTGTGTTACCTCATGGCGATCAGCAATATTTGTTCAGC CCAAGTAGAGAAATGCCTACTTTTTCAGGTACATTGGAAGGTCATCTGATTCCTATGGCAATTCTTTTA GGACAAACCCAAAGTAATAGTGATACCATGCCACCTGCTGGAGTGATTGTCAGCAAGCCACACCCTGTA ACTGTGACTACTTCTATTCCTCCATCATCTCGAAAAGTAGAAACTGGAGTAAAGAAACCTAACATAGCC ATTGTAGAAATGAAGTCAGAAAAAAAGGATCCTCCTCAGCTTACTGTGCAGGTATTACCCAGTGTAGAT ATTGACAGCATTTCAAATAGTAGTGCTGATGTCCTTTCACCTCTGTCTAGCCCCAAAGAAGCATCTCTT CCTCCTGTGCAAACTTGGATAAAGACTCCAGAAATTATGAAGGTAGATGAAGAAGAGGTGAAGTTTCCA GGAACTAACTTTGATGAAATAATCGATGTCATACAGGAAGAAGAAAAATGTGATGAAATTCCAGACTCT GAACCAATTCTGGAGTTTAACAGAAGTGTTAAAGCTGATTCTACAAAATATAATGGTCCTCCATTTCCG CCAGTTGCTTCTACTTTTCAGCCCACTGCTGATATTCTGGATAAAGTAATTGAGAGAAAAGAAACACTG GAAAATAGCTTAATTCAATGGGTAGAGCAAGAAATAATGTCAAGAATTATCTCTGGGCTCTTTCCAGTC CAGCAACAGATTGCACCTAGTATCAGTGTTTCAGTCAGTGAGACAAGTGAACCACTGACTTCTGACATT GTGGAAGGAACAAGCAGTGGCGCCCTCCAGCTTTTTGTTGATGCTGGTGTTCCTGTGAACTCAAATGTG ATTAAACATTTTGTTAACGAAGCTCTTGCTGAGACCATTGCTGTCATGCTGGGTGACAGAGAAGCAAAG AAGCAAGGTCCTGTTGCTACAGGTGTTTCTGGGGATGCTTCAACAAATGAAACATATTTGCCGGCAAGA GTGTGCACCCCACTGCCTACCCCACAGCCTACGCCTCCTTGCTCACCTTCATCACCTGCTAAGGAGTGT GTTTTGGTAAAGACTCCAGATTCTTCTCCCTGTGATTCGGATCATGATATGGCTTTTCCTGTGAAAGAA ATATGTGCTGAAAAAGGAGATGATATGCCTGCCATCATGCTTGTTAATACTCCAACAGTTACCCCTACT ACTACACCTCCTCCAGCGGCGGCAGTTTTTACCCCAACTTTGTCAGATATTTCCATTGATAAATTGAAG GTATCAAGCCCAGAGCTTCCCAAGCCATGGGGTGATGGAGACCTGCCACTGGAAGAAGAGAACCCTAAC TCACCTCAAGAAGAACTTCATCCAAGAGCTATTGTAATGTCTGTGGCTAAGGATGAAGAACCAGAGAGT ATGGATTTCCCTGCTCAGCCTCCACCTCCAGAGCCAGTTCCCTTTATGCCATTTCCTGCCGGCACCAAG GCCCCTTCCCCCTCACAGATGCCAGGTTCTGATTCATCAACACTGGAGAGCACATTGAGTGTTACTGTC ACTGAAACTGAAACTTTAGATAAACCCATCTCTGAAGGAGAGATTTTATTTAGCTGTGGTCAAAAATTG GCCCCCAAGATTTTAGAAGATATAGGACTGTACCTGACAAACCTTAATGATAGCTTATCCAGCACTCTG CATGATGCCGTTGAAATGGAGGATGATCCTCCTAGTGAAGGGCAAGTGATTAGGATGTCCCATAAAAAA TTTCATGCAGATGCAATTCTTTCTTTTGCTAAACAAAACCAGGAGTCAGCAGTTTCCCAGCAAGCAGTC TATCATTCAGAGGACTTGGAAAACAGTGTGGGTGAACTTAGTGAAGGACAAAGACCCCAGCTAACAGCG GCAGCAGAGAACATCTTAATGGGACATTCTCTCTATATGCAGCCACCTGTCACTAATACACAGTCTTTG GATCAACAATGTGATCCTAAACCATTATCTCGGCAATTTGACACAGTTTCAGGTAGTATTTATGAAGAT TCATGTGCTAGTCATGGTCCAATGAGTTTGGGAGAATTGGAGTTGGAGCCAAATTCTAAGCTGGTTCTT CCCACAACACTTCTGACAGCACAAGAAAATGATGTTAATTTACCAGTAGCCGCTGAAGATTTTTCCCAG TACCAACTAAAGCAAAATCAGGATGTTAAGCAAGTTGAACACAAACCATCACAAAGTTACCTACGTGTT AGAAATAAATCTGATATTGCACCTTCACAGCAACAAGTTTCACCAGGTGATATGGATCGGACACAAATT GAGCTTAATCCGTACCTCACATGTGTATTTTCAGGTGGGAAAGCAGTGCCACTCTCCGCTTCACAGATG CCCCCTGCCAAGATGTCAGTGATGCTGCCGTCAGTGAACCTCGAGGACTGCTCTCAGTCTCTGAGTCTC AGCACAATGCAGGAGGACATGGAGTCTTCGGGGGCAGATACCTTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001244190
Insert Size	4602 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001244190.1
RefSeq Size	5801 bp
RefSeq ORF	4602 bp
Locus ID	9786
UniProt ID	Q9BVV6
MW	169.3 kDa
Gene Summary	This gene encodes a conserved centrosomal protein that functions in ciliogenesis and responds to hedgehog signaling. Mutations in this gene causes Joubert syndrome 23. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Aug 2016] Transcript Variant: This variant (2) lacks an alternate segment in the 5' coding region, uses a downstream start codon, and lacks several alternate in-frame exons, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

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