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KIAA0586 (NM_001244190) Human Tagged ORF Clone
CAT＃: RG235318
- TrueORF®
KIAA0586 (tGFP-tagged) - Homo sapiens KIAA0586 (KIAA0586), transcript variant 2
ORF Plasmid: DDK
"NM_001244190" in other vectors (2)
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CNY 16,820.00
货期*
14周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | JBTS23; SRTD14; Talpid3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235318 representing NM_001244190
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGTGAAGAGACTTCGTGAGGTAGTTTCTCAAAATCATGGAGATCATTTGGTTTTGCTGAAAGATG AGTTGCCCTGTGTTCCTCCGGCATTGTCTGCAAATAAACGTCTTCCTGTTGGAACGGGGACTAGTTTGAA TGGAACATCACGTGGTTCATCAGACTTAACTTCTGCTAGAAATTGTTACCAGCCTCTATTAGAAAATCCC ATGGTGTCAGAAAGTGATTTTTCTAAAGACGTTGCAGTGCAAGTGTTGCCTTTGGATAAAATAGAAGAGA ACAACAAGCAAAAAGCAAATGACATCTTCATTTCTCAGTATACAATGGGACAGAAAGATGCTCTAAGAAC AGTTTTAAAGCAAAAAGCTCAAAGCATGCCTGTTTTTAAGGAAGTAAAGGTACATCTGTTAGAAGATGCA GGCATAGAGAAGGATGCTGTTACTCAGGAGACTAGAATTTCACCCAGTGGAATTGATTCAGCTACAACCG TGGCTGCAGCAACTGCTGCTGCCATTGCAACCGCAGCTCCGTTGATAAAGGTGCAGAGTGATTTGGAAGC AAAAGTCAATTCTGTTACAGAATTACTTAGTAAATTACAGGAGACTGATAAACACCTGCAACGTGTTACA GAGCAGCAAACAAGCATTCAGAGGAAACAAGAGAAATTACATTGTCATGATCACGAAAAGCAAATGAATG TGTTTATGGAGCAGCACATAAGGCATCTTGAAAAGTTACAACAACAACAAATAGATATTCAGACTCATTT TATTAGTGCTGCACTCAAGACTAGTAGTTTTCAGCCTGTTAGTATGCCCTCCTCCAGAGCAGTGGAAAAG TATTCCGTAAAACCAGAACACCCTAATCTTGGTAGCTGTAATCCATCTTTATATAACACATTTGCTTCCA AACAAGCACCTTTAAAAGAAGTTGAAGATACGAGTTTTGATAAACAGAAATCTCCTTTGGAGACACCAGC ACCTCGCAGATTTGCTCCTGTACCTGTTTCAAGGGATGATGAACTATCAAAGAGGGAAAATCTTTTGGAA GAAAAAGAAAATATGGAAGTGTCGTGTCACAGAGGAAATGTAAGACTATTGGAACAAATTTTGAATAATA ATGATTCTTTGACAAGAAAAAGTGAATCATCAAACACCACCTCACTAACTAGGTCAAAAATAGGATGGAC TCCTGAGAAAACAAACAGATTTCCTTCCTGTGAAGAGCTAGAAACAACTAAAGTGACTATGCAGAAGTCT GATGATGTTCTTCATGACCTTGGCCAAAAAGAGAAAGAAACAAATAGCATGGTCCAGCCAAAAGAATCTC TGAGTATGTTGAAGCTTCCAGATCTTCCACAGAATTCTGTTAAGCTTCAAACAACCAATACAACAAGATC TGTATTGAAAGATGCTGAGAAGATTTTGAGAGGAGTACAAAACAATAAAAAAGTACTTGAAGAAAACCTG GAAGCTATTATTCGTGCAAAAGATGGAGCTGCCATGTATTCGCTTATCAATGCTTTATCTACCAACAGAG AGATGTCAGAGAAAATTAGGATCAGAAAGACAGTGGATGAATGGATTAAAACTATTTCTGCAGAAATTCA GGATGAACTGTCAAGAACAGATTATGAACAAAAAAGATTTGATCAGAAGAATCAGAGAACCAAGAAAGGT CAGAATATGACTAAAGATATTAGAACCAACACACAAGATAAAACTGTCAACAAATCTGTAATTCCAAGAA AACATTCTCAAAAGCAAATAGAAGAGCATTTTAGAAATCTACCTATGAGGGGCATGCCTGCTTCAAGTTT ACAGAAAGAGAGAAAGGAAGGGCTTTTGAAAGCAACCACAGTAATACAAGATGAAGATTATATGTTACAA GTCTATGGAAAGCCAGTTTATCAGGGCCATCGAAGCACTCTTAAAAAAGGACCATATCTCAGATTTAATT CTCCATCTCCTAAGTCCAGACCACAGAGACCAAAAGTAATAGAACGAGTTAAAGGCACTAAGGTAAAGTC AATAAGAACACAGACTGACTTCTATGCAACAAAACCTAAGAAGATGGATTCTAAAATGAAACATTCTGTT CCTGTGTTACCTCATGGCGATCAGCAATATTTGTTCAGCCCAAGTAGAGAAATGCCTACTTTTTCAGGTA CATTGGAAGGTCATCTGATTCCTATGGCAATTCTTTTAGGACAAACCCAAAGTAATAGTGATACCATGCC ACCTGCTGGAGTGATTGTCAGCAAGCCACACCCTGTAACTGTGACTACTTCTATTCCTCCATCATCTCGA AAAGTAGAAACTGGAGTAAAGAAACCTAACATAGCCATTGTAGAAATGAAGTCAGAAAAAAAGGATCCTC CTCAGCTTACTGTGCAGGTATTACCCAGTGTAGATATTGACAGCATTTCAAATAGTAGTGCTGATGTCCT TTCACCTCTGTCTAGCCCCAAAGAAGCATCTCTTCCTCCTGTGCAAACTTGGATAAAGACTCCAGAAATT ATGAAGGTAGATGAAGAAGAGGTGAAGTTTCCAGGAACTAACTTTGATGAAATAATCGATGTCATACAGG AAGAAGAAAAATGTGATGAAATTCCAGACTCTGAACCAATTCTGGAGTTTAACAGAAGTGTTAAAGCTGA TTCTACAAAATATAATGGTCCTCCATTTCCGCCAGTTGCTTCTACTTTTCAGCCCACTGCTGATATTCTG GATAAAGTAATTGAGAGAAAAGAAACACTGGAAAATAGCTTAATTCAATGGGTAGAGCAAGAAATAATGT CAAGAATTATCTCTGGGCTCTTTCCAGTCCAGCAACAGATTGCACCTAGTATCAGTGTTTCAGTCAGTGA GACAAGTGAACCACTGACTTCTGACATTGTGGAAGGAACAAGCAGTGGCGCCCTCCAGCTTTTTGTTGAT GCTGGTGTTCCTGTGAACTCAAATGTGATTAAACATTTTGTTAACGAAGCTCTTGCTGAGACCATTGCTG TCATGCTGGGTGACAGAGAAGCAAAGAAGCAAGGTCCTGTTGCTACAGGTGTTTCTGGGGATGCTTCAAC AAATGAAACATATTTGCCGGCAAGAGTGTGCACCCCACTGCCTACCCCACAGCCTACGCCTCCTTGCTCA CCTTCATCACCTGCTAAGGAGTGTGTTTTGGTAAAGACTCCAGATTCTTCTCCCTGTGATTCGGATCATG ATATGGCTTTTCCTGTGAAAGAAATATGTGCTGAAAAAGGAGATGATATGCCTGCCATCATGCTTGTTAA TACTCCAACAGTTACCCCTACTACTACACCTCCTCCAGCGGCGGCAGTTTTTACCCCAACTTTGTCAGAT ATTTCCATTGATAAATTGAAGGTATCAAGCCCAGAGCTTCCCAAGCCATGGGGTGATGGAGACCTGCCAC TGGAAGAAGAGAACCCTAACTCACCTCAAGAAGAACTTCATCCAAGAGCTATTGTAATGTCTGTGGCTAA GGATGAAGAACCAGAGAGTATGGATTTCCCTGCTCAGCCTCCACCTCCAGAGCCAGTTCCCTTTATGCCA TTTCCTGCCGGCACCAAGGCCCCTTCCCCCTCACAGATGCCAGGTTCTGATTCATCAACACTGGAGAGCA CATTGAGTGTTACTGTCACTGAAACTGAAACTTTAGATAAACCCATCTCTGAAGGAGAGATTTTATTTAG CTGTGGTCAAAAATTGGCCCCCAAGATTTTAGAAGATATAGGACTGTACCTGACAAACCTTAATGATAGC TTATCCAGCACTCTGCATGATGCCGTTGAAATGGAGGATGATCCTCCTAGTGAAGGGCAAGTGATTAGGA TGTCCCATAAAAAATTTCATGCAGATGCAATTCTTTCTTTTGCTAAACAAAACCAGGAGTCAGCAGTTTC CCAGCAAGCAGTCTATCATTCAGAGGACTTGGAAAACAGTGTGGGTGAACTTAGTGAAGGACAAAGACCC CAGCTAACAGCGGCAGCAGAGAACATCTTAATGGGACATTCTCTCTATATGCAGCCACCTGTCACTAATA CACAGTCTTTGGATCAACAATGTGATCCTAAACCATTATCTCGGCAATTTGACACAGTTTCAGGTAGTAT TTATGAAGATTCATGTGCTAGTCATGGTCCAATGAGTTTGGGAGAATTGGAGTTGGAGCCAAATTCTAAG CTGGTTCTTCCCACAACACTTCTGACAGCACAAGAAAATGATGTTAATTTACCAGTAGCCGCTGAAGATT TTTCCCAGTACCAACTAAAGCAAAATCAGGATGTTAAGCAAGTTGAACACAAACCATCACAAAGTTACCT ACGTGTTAGAAATAAATCTGATATTGCACCTTCACAGCAACAAGTTTCACCAGGTGATATGGATCGGACA CAAATTGAGCTTAATCCGTACCTCACATGTGTATTTTCAGGTGGGAAAGCAGTGCCACTCTCCGCTTCAC AGATGCCCCCTGCCAAGATGTCAGTGATGCTGCCGTCAGTGAACCTCGAGGACTGCTCTCAGTCTCTGAG TCTCAGCACAATGCAGGAGGACATGGAGTCTTCGGGGGCAGATACCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235318 representing NM_001244190
Red=Cloning site Green=Tags(s) MPVKRLREVVSQNHGDHLVLLKDELPCVPPALSANKRLPVGTGTSLNGTSRGSSDLTSARNCYQPLLENP MVSESDFSKDVAVQVLPLDKIEENNKQKANDIFISQYTMGQKDALRTVLKQKAQSMPVFKEVKVHLLEDA GIEKDAVTQETRISPSGIDSATTVAAATAAAIATAAPLIKVQSDLEAKVNSVTELLSKLQETDKHLQRVT EQQTSIQRKQEKLHCHDHEKQMNVFMEQHIRHLEKLQQQQIDIQTHFISAALKTSSFQPVSMPSSRAVEK YSVKPEHPNLGSCNPSLYNTFASKQAPLKEVEDTSFDKQKSPLETPAPRRFAPVPVSRDDELSKRENLLE EKENMEVSCHRGNVRLLEQILNNNDSLTRKSESSNTTSLTRSKIGWTPEKTNRFPSCEELETTKVTMQKS DDVLHDLGQKEKETNSMVQPKESLSMLKLPDLPQNSVKLQTTNTTRSVLKDAEKILRGVQNNKKVLEENL EAIIRAKDGAAMYSLINALSTNREMSEKIRIRKTVDEWIKTISAEIQDELSRTDYEQKRFDQKNQRTKKG QNMTKDIRTNTQDKTVNKSVIPRKHSQKQIEEHFRNLPMRGMPASSLQKERKEGLLKATTVIQDEDYMLQ VYGKPVYQGHRSTLKKGPYLRFNSPSPKSRPQRPKVIERVKGTKVKSIRTQTDFYATKPKKMDSKMKHSV PVLPHGDQQYLFSPSREMPTFSGTLEGHLIPMAILLGQTQSNSDTMPPAGVIVSKPHPVTVTTSIPPSSR KVETGVKKPNIAIVEMKSEKKDPPQLTVQVLPSVDIDSISNSSADVLSPLSSPKEASLPPVQTWIKTPEI MKVDEEEVKFPGTNFDEIIDVIQEEEKCDEIPDSEPILEFNRSVKADSTKYNGPPFPPVASTFQPTADIL DKVIERKETLENSLIQWVEQEIMSRIISGLFPVQQQIAPSISVSVSETSEPLTSDIVEGTSSGALQLFVD AGVPVNSNVIKHFVNEALAETIAVMLGDREAKKQGPVATGVSGDASTNETYLPARVCTPLPTPQPTPPCS PSSPAKECVLVKTPDSSPCDSDHDMAFPVKEICAEKGDDMPAIMLVNTPTVTPTTTPPPAAAVFTPTLSD ISIDKLKVSSPELPKPWGDGDLPLEEENPNSPQEELHPRAIVMSVAKDEEPESMDFPAQPPPPEPVPFMP FPAGTKAPSPSQMPGSDSSTLESTLSVTVTETETLDKPISEGEILFSCGQKLAPKILEDIGLYLTNLNDS LSSTLHDAVEMEDDPPSEGQVIRMSHKKFHADAILSFAKQNQESAVSQQAVYHSEDLENSVGELSEGQRP QLTAAAENILMGHSLYMQPPVTNTQSLDQQCDPKPLSRQFDTVSGSIYEDSCASHGPMSLGELELEPNSK LVLPTTLLTAQENDVNLPVAAEDFSQYQLKQNQDVKQVEHKPSQSYLRVRNKSDIAPSQQQVSPGDMDRT QIELNPYLTCVFSGGKAVPLSASQMPPAKMSVMLPSVNLEDCSQSLSLSTMQEDMESSGADTF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001244190 |
| ORF Size | 4599 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001244190.2 |
| RefSeq Size | 5801 bp |
| RefSeq ORF | 4602 bp |
| Locus ID | 9786 |
| UniProt ID | Q9BVV6 |
| Gene Summary | This gene encodes a conserved centrosomal protein that functions in ciliogenesis and responds to hedgehog signaling. Mutations in this gene causes Joubert syndrome 23. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Aug 2016] |
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