RAD54B (NM_001205263) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RDH54
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331648 representing NM_001205263. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATTTGTGGAAAAGAAATAGAAGTCATGGGTGTAATCTCTCCAGATGACTTCAGCAGTGGCAGGTGT TTTCAGCTTGGAGGAGGAAGTACTGCTATCTCGCATTCTTCTCAGGTTGCCAGGAAATGTTTCTCTAAC CCTTTCAAAAGTGTTTGTAAACCAAGTTCAAAGGAAAATAGACAGAATGATTTCCAAAATTGCAAACCA CGCCATGACCCATATACGCCAAATTCCCTCGTTATGCCACGACCAGATAAGAATCACCAGTGGGTATTC AATAAGAACTGTTTCCCTCTTGTGGATGTAGTGATTGATCCTTACCTTGTATATCATCTTCGACCACAT CAGAAAGAAGGAATCATATTCCTTTATGAATGTGTAATGGGAATGAGAATGAATGGCAGATGTGGAGCT ATTCTTGCTGATGAAATGGGTTTAGGGAAGACATTGCAATGTATTTCGCTCATCTGGACCCTGCAGTGT CAGGGACCCTATGGAGGCAAGCCAGTAATAAAGAAGACACTAATTGTCACACCTGGAAGCTTGGTGAAT AATTGGAAGAAAGAATTTCAAAAATGGCTAGGAAGTGAAAGGATCAAGATATTTACTGTTGATCAGGAC CACAAAGTTGAAGAATTCATCAAGTCTATATTTTATTCTGTTCTTATTATCAGTTATGAAATGTTACTT CGTTCCCTGGATCAAATTAAGAATATAAAATTTGATCTTCTAATCTGTGACGAGGGGCATCGTTTGAAG AACAGTGCCATTAAGACAACTACAGCCCTCATTAGCCTTTCTTGTGAGAAAAGAATAATTCTAACTGGT ACTCCAATTCAGAATGATCTGCAAGAATTTTTTGCATTAATTGATTTTGTAAATCCAGGAATATTAGGC TCTTTGTCATCTTATAGGAAAATATATGAAGAACCCATCATTTTATCGAGAGAACCTTCTGCTTCTGAG GAAGAAAAGGAGTTAGGAGAAAGAAGAGCAGCTGAACTTACTTGCCTCACTGGACTCTTTATCCTTAGA AGAACCCAAGAAATTATAAATAAATATCTCCCACCTAAAATAGAGAATGTTGTCTTTTGCCGACCAGGA GCACTACAGATTGAGCTTTATCGAAAGCTGTTAAATTCTCAGGTTGTCAGGTTCTGCCTTCAAGGGTTG TTGGAAAATAGTCCCCATCTAATATGTATAGGAGCTCTTAAAAAACTGTGCAATCACCCCTGCCTTTTG TTCAACTCTATAAAGGAAAAGGAATGTAGCTCAACTTGTGATAAAAATGAAGAAAAGAGTCTATACAAA GGCTTGCTAAGTGTGTTTCCTGCTGACTACAACCCTCTCCTGTTTACTGAAAAGGAGTCAGGAAAACTA CAGGTGTTGTCCAAGCTCTTAGCGGTTATCCACGAACTTCGACCTACTGAAAAGGTGGTGTTGGTATCC AACTATACACAAACCTTGAACATTTTACAAGAAGTATGTAAGCGTCATGGATATGCTTATACAAGACTT GATGGACAAACACCAATCTCTCAAAGGCAGCAGATTGTTGATGGCTTTAACAGTCAACACTCTTCTTTT TTTATTTTTTTGTTAAGTTCAAAAGCTGGTGGTGTAGGACTTAACCTCATTGGAGGATCTCACTTAATT CTCTATGACATTGATTGGAATCCAGCCACTGACATTCAGGCAATGTCTAGAGTATGGAGAGATGGTCAG AAATATCCTGTACATATTTACAGACTCCTAACTACAGGTACAATAGAAGAAAAGATCTATCAAAGGCAG ATCAGTAAGCAAGGTCTTTGTGGGGCAGTTGTCGACCTCACCAAGACATCTGAACATATTCAGTTTTCA GTAGAAGAACTTAAAAATTTGTTCACATTACATGAAAGTTCAGATTGTGTTACTCATGATCTGCTTGAC TGTGAGTGTACAGGAGAAGAAGTTCATACAGGTGATTCGTTGGAAAAATTCATTGTCTCTAGAGATTGT CAGCTTGGTCCACATCACCAGAAATCTAACTCCCTGAAACCTCTTTCTATGTCCCAGCTGAAGCAATGG AAACATTTTTCTGGAGATCATTTAAATCTTACAGATCCTTTTCTTGAAAGAATAACAGAAAATGTGTCA TTCATTTTTCAGAATATAACCACTCAAGCTACTGGCACATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001205263
Insert Size	2181 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001205263.1
RefSeq Size	3097 bp
RefSeq ORF	2181 bp
Locus ID	25788
UniProt ID	Q9Y620
Protein Families	Druggable Genome
Protein Pathways	Homologous recombination
MW	82.4 kDa
Gene Summary	The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) lacks 5' several exons but uses an alternate 5' exon, and it thus differs in the 5' UTR and uses a downstream start codon, compared to variant 1. The encoded isoform (3) is shorter at the N-terminus, compared to isoform 1.

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