RAD54B (NM_001205263) Human Tagged ORF Clone
CAT#: RG234779
- TrueORF®
RAD54B (tGFP-tagged) - Homo sapiens RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 3
ORF Plasmid: DDK
"NM_001205263" in other vectors (2)
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CNY 7,980.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | RDH54 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234779 representing NM_001205263
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTTGTGGAAAAGAAATAGAAGTCATGGGTGTAATCTCTCCAGATGACTTCAGCAGTGGCAGGTGTT TTCAGCTTGGAGGAGGAAGTACTGCTATCTCGCATTCTTCTCAGGTTGCCAGGAAATGTTTCTCTAACCC TTTCAAAAGTGTTTGTAAACCAAGTTCAAAGGAAAATAGACAGAATGATTTCCAAAATTGCAAACCACGC CATGACCCATATACGCCAAATTCCCTCGTTATGCCACGACCAGATAAGAATCACCAGTGGGTATTCAATA AGAACTGTTTCCCTCTTGTGGATGTAGTGATTGATCCTTACCTTGTATATCATCTTCGACCACATCAGAA AGAAGGAATCATATTCCTTTATGAATGTGTAATGGGAATGAGAATGAATGGCAGATGTGGAGCTATTCTT GCTGATGAAATGGGTTTAGGGAAGACATTGCAATGTATTTCGCTCATCTGGACCCTGCAGTGTCAGGGAC CCTATGGAGGCAAGCCAGTAATAAAGAAGACACTAATTGTCACACCTGGAAGCTTGGTGAATAATTGGAA GAAAGAATTTCAAAAATGGCTAGGAAGTGAAAGGATCAAGATATTTACTGTTGATCAGGACCACAAAGTT GAAGAATTCATCAAGTCTATATTTTATTCTGTTCTTATTATCAGTTATGAAATGTTACTTCGTTCCCTGG ATCAAATTAAGAATATAAAATTTGATCTTCTAATCTGTGACGAGGGGCATCGTTTGAAGAACAGTGCCAT TAAGACAACTACAGCCCTCATTAGCCTTTCTTGTGAGAAAAGAATAATTCTAACTGGTACTCCAATTCAG AATGATCTGCAAGAATTTTTTGCATTAATTGATTTTGTAAATCCAGGAATATTAGGCTCTTTGTCATCTT ATAGGAAAATATATGAAGAACCCATCATTTTATCGAGAGAACCTTCTGCTTCTGAGGAAGAAAAGGAGTT AGGAGAAAGAAGAGCAGCTGAACTTACTTGCCTCACTGGACTCTTTATCCTTAGAAGAACCCAAGAAATT ATAAATAAATATCTCCCACCTAAAATAGAGAATGTTGTCTTTTGCCGACCAGGAGCACTACAGATTGAGC TTTATCGAAAGCTGTTAAATTCTCAGGTTGTCAGGTTCTGCCTTCAAGGGTTGTTGGAAAATAGTCCCCA TCTAATATGTATAGGAGCTCTTAAAAAACTGTGCAATCACCCCTGCCTTTTGTTCAACTCTATAAAGGAA AAGGAATGTAGCTCAACTTGTGATAAAAATGAAGAAAAGAGTCTATACAAAGGCTTGCTAAGTGTGTTTC CTGCTGACTACAACCCTCTCCTGTTTACTGAAAAGGAGTCAGGAAAACTACAGGTGTTGTCCAAGCTCTT AGCGGTTATCCACGAACTTCGACCTACTGAAAAGGTGGTGTTGGTATCCAACTATACACAAACCTTGAAC ATTTTACAAGAAGTATGTAAGCGTCATGGATATGCTTATACAAGACTTGATGGACAAACACCAATCTCTC AAAGGCAGCAGATTGTTGATGGCTTTAACAGTCAACACTCTTCTTTTTTTATTTTTTTGTTAAGTTCAAA AGCTGGTGGTGTAGGACTTAACCTCATTGGAGGATCTCACTTAATTCTCTATGACATTGATTGGAATCCA GCCACTGACATTCAGGCAATGTCTAGAGTATGGAGAGATGGTCAGAAATATCCTGTACATATTTACAGAC TCCTAACTACAGGTACAATAGAAGAAAAGATCTATCAAAGGCAGATCAGTAAGCAAGGTCTTTGTGGGGC AGTTGTCGACCTCACCAAGACATCTGAACATATTCAGTTTTCAGTAGAAGAACTTAAAAATTTGTTCACA TTACATGAAAGTTCAGATTGTGTTACTCATGATCTGCTTGACTGTGAGTGTACAGGAGAAGAAGTTCATA CAGGTGATTCGTTGGAAAAATTCATTGTCTCTAGAGATTGTCAGCTTGGTCCACATCACCAGAAATCTAA CTCCCTGAAACCTCTTTCTATGTCCCAGCTGAAGCAATGGAAACATTTTTCTGGAGATCATTTAAATCTT ACAGATCCTTTTCTTGAAAGAATAACAGAAAATGTGTCATTCATTTTTCAGAATATAACCACTCAAGCTA CTGGCACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234779 representing NM_001205263
Red=Cloning site Green=Tags(s) MICGKEIEVMGVISPDDFSSGRCFQLGGGSTAISHSSQVARKCFSNPFKSVCKPSSKENRQNDFQNCKPR HDPYTPNSLVMPRPDKNHQWVFNKNCFPLVDVVIDPYLVYHLRPHQKEGIIFLYECVMGMRMNGRCGAIL ADEMGLGKTLQCISLIWTLQCQGPYGGKPVIKKTLIVTPGSLVNNWKKEFQKWLGSERIKIFTVDQDHKV EEFIKSIFYSVLIISYEMLLRSLDQIKNIKFDLLICDEGHRLKNSAIKTTTALISLSCEKRIILTGTPIQ NDLQEFFALIDFVNPGILGSLSSYRKIYEEPIILSREPSASEEEKELGERRAAELTCLTGLFILRRTQEI INKYLPPKIENVVFCRPGALQIELYRKLLNSQVVRFCLQGLLENSPHLICIGALKKLCNHPCLLFNSIKE KECSSTCDKNEEKSLYKGLLSVFPADYNPLLFTEKESGKLQVLSKLLAVIHELRPTEKVVLVSNYTQTLN ILQEVCKRHGYAYTRLDGQTPISQRQQIVDGFNSQHSSFFIFLLSSKAGGVGLNLIGGSHLILYDIDWNP ATDIQAMSRVWRDGQKYPVHIYRLLTTGTIEEKIYQRQISKQGLCGAVVDLTKTSEHIQFSVEELKNLFT LHESSDCVTHDLLDCECTGEEVHTGDSLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNL TDPFLERITENVSFIFQNITTQATGT TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001205263 |
ORF Size | 2178 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001205263.2 |
RefSeq Size | 3097 bp |
RefSeq ORF | 2181 bp |
Locus ID | 25788 |
UniProt ID | Q9Y620 |
Protein Families | Druggable Genome |
Protein Pathways | Homologous recombination |
Gene Summary | The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq, Jul 2008] |
Documents
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FAQs |
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