Ankyrin G (ANK3) (NM_001204403) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ANKYRIN-G; MRT37
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331570 representing NM_001204403. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCTCCTCCGCCTCCTCCTCTCCCGCGGGCACCGAGGACTCCGCTCCTGCCCAGGGTGGCTTTGGA TCAGATTATTCACGAAGCTCTAGAAAGTCTGATGCCAATGCAAGTTACTTAAGAGCAGCTCGAGCTGGA CACCTTGAAAAGGCCCTCGACTACATAAAAAATGGAGTTGACATCAACATTTGCAATCAGAATGGGTTG AACGCTCTCCACCTTGCTTCCAAAGAAGGCCATGTAGAGGTTGTTTCTGAGCTGCTGCAGAGAGAAGCC AATGTGGATGCAGCTACAAAGAAAGGAAACACAGCATTGCACATCGCATCTTTGGCTGGGCAAGCAGAG GTGGTAAAAGTCTTGGTTACAAATGGAGCCAATGTCAATGCACAATCTCAGAATGGTTTCACGCCATTG TATATGGCAGCCCAGGAAAATCACCTGGAAGTTGTCAAGTTTCTTCTTGACAATGGTGCAAGCCAGAGC CTAGCCACAGAGGATGGCTTCACACCATTGGCAGTGGCTTTGCAACAAGGTCACGACCAAGTCGTTTCG CTCCTGCTAGAGAATGACACCAAAGGAAAAGTGCGTCTCCCAGCTCTTCATATCGCGGCCCGAAAAGAC GACACGAAAGCCGCCGCCCTGCTGCTGCAGAATGACAACAATGCAGATGTGGAATCAAAGAGTGGCTTC ACTCCGCTCCACATAGCTGCTCACTATGGAAATATCAATGTAGCCACGTTGCTGTTAAACCGAGCGGCT GCTGTGGATTTCACCGCAAGGAATGACATCACTCCTTTACATGTTGCATCAAAAAGAGGAAATGCAAAT ATGGTAAAACTATTGCTCGATCGAGGAGCTAAAATCGATGCCAAAACCAGGGATGGTCTGACACCACTG CACTGTGGAGCAAGGAGTGGCCACGAGCAGGTGGTAGAAATGTTGCTTGATCGAGCTGCCCCCATTCTT TCAAAAACCAAGAATGGATTATCTCCATTGCACATGGCCACACAAGGGGATCATTTAAACTGCGTCCAG CTTCTCCTCCAGCATAATGTACCCGTGGATGATGTCACCAATGACTACCTGACTGCCCTACACGTGGCT GCCCACTGTGGCCATTACAAAGTTGCCAAGGTTCTCTTGGATAAGAAAGCTAACCCCAATGCCAAAGCC CTGAATGGCTTTACCCCTCTTCATATTGCCTGCAAGAAGAATCGAATTAAAGTAATGGAACTCCTTCTG AAACACGGTGCATCCATCCAAGCTGTAACCGAGTCGGGCCTTACCCCAATCCATGTTGCTGCCTTCATG GGGCATGTAAATATTGTATCACAACTAATGCATCATGGAGCCTCACCAAACACCACCAATGTGAGAGGA GAAACAGCACTGCACATGGCAGCTCGCTCCGGCCAAGCTGAAGTTGTGCGGTATCTGGTACAAGACGGA GCTCAGGTAGAAGCTAAAGCTAAGGATGACCAAACACCACTCCACATTTCAGCCCGACTGGGGAAAGCA GACATAGTACAACAGCTGTTGCAGCAAGGGGCATCTCCAAATGCAGCCACAACTTCTGGGTACACCCCA CTTCACCTTTCCGCCCGAGAGGGGCATGAGGATGTGGCCGCGTTCCTTTTGGATCATGGAGCGTCTTTA TCTATAACAACAAAGAAAGGATTTACTCCTCTTCATGTGGCAGCAAAATATGGAAAGCTTGAAGTCGCC AATCTCCTGCTACAGAAAAGTGCATCTCCAGATGCTGCTGGGAAGAGCGGGCTAACACCACTGCATGTA GCTGCACATTACGATAATCAGAAAGTGGCCCTTCTGCTTTTGGACCAAGGAGCCTCACCTCACGCAGCC GCAAAGAATGGTTATACGCCACTGCACATCGCTGCCAAAAAGAACCAGATGGACATAGCGACAACTCTG CTGGAATATGGTGCTGATGCCAACGCAGTTACCCGGCAAGGAATTGCTTCCGTCCATCTCGCAGCTCAG GAAGGGCACGTGGACATGGTGTCGCTGCTCCTCGGTAGAAATGCGAATGTGAACCTGAGCAATAAGAGC GGCCTGACCCCACTCCATTTGGCTGCTCAAGAAGATCGAGTGAATGTGGCAGAAGTCCTCGTAAACCAA GGGGCTCATGTGGACGCCCAGACAAAGATGGGATACACACCACTGCATGTGGGCTGCCACTATGGAAAT ATCAAGATTGTTAATTTCCTGCTCCAGCATTCTGCAAAAGTTAATGCCAAAACAAAGAATGGGTATACG CCATTACATCAAGCAGCACAGCAGGGGCATACGCATATAATAAATGTCTTACTTCAGAACAACGCCTCC CCCAATGAACTCACTGTGAATGGGAATACTGCCCTTGGCATTGCCCGGCGCCTCGGCTACATCTCAGTA GTGGACACCCTGAAGATAGTGACCGAAGAGACCATGACCACAACTACTGTCACAGAGAAGCACAAAATG AATGTTCCAGAAACGATGAATGAAGTTCTTGATATGTCTGATGATGAAGTTCGTAAAGCCAATGCCCCT GAAATGCTCAGTGATGGCGAATATATCTCAGATGTTGAAGAAGGTGAAGATGCAATGACCGGGGACACA GACAAATATCTTGGGCCACAGGACCTTAAGGAATTGGGTGATGATTCCCTGCCTGCAGAGGGTTACATG GGCTTTAGTCTCGGAGCGCGTTCTGCCAGCCTCCGCTCCTTCAGTTCGGATAGGTCTTACACCTTGAAC AGAAGCTCCTATGCACGGGACAGCATGATGATTGAAGAACTCCTTGTGCCATCCAAAGAGCAGCATCTA ACATTCACAAGGGAATTTGATTCAGATTCTCTTAGACATTACAGCTGGGCTGCAGACACCTTAGACAAT GTCAATCTTGTTTCAAGCCCCATTCATTCTGGGTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGGC TCCATGAGAGGAAGCCGTCATCACGGGATGAGAATCATCATTCCTCCACGCAAGTGTACGGCCCCCACT CGAATCACCTGCCGTTTGGTAAAGAGACATAAACTGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGA TTAGCCAGTAGGCTGGTAGAAATGGGTCCTGCAGGGGCACAATTTTTAGGCCCTGTCATAGTGGAAATC CCTCACTTTGGGTCCATGAGAGGAAAAGAGAGAGAACTCATTGTTCTTCGAAGTGAAAATGGTGAAACT TGGAAGGAGCATCAGTTTGACAGCAAAAATGAAGATTTAACCGAGTTACTTAATGGCATGGATGAAGAA CTTGATAGCCCAGAAGAGTTAGGGAAAAAGCGTATCTGCAGGATTATCACGAAAGATTTCCCCCAGTAT TTTGCAGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAGATTGGTCCTGAAGGTGGAATTCTGAGCAGC ACCACAGTGCCCCTTGTTCAAGCATCTTTCCCAGAGGGTGCCCTAACTAAAAGAATTCGAGTGGGCCTC CAGGCCCAGCCTGTTCCAGATGAAATTGTGAAAAAGATCCTTGGAAACAAAGCAACTTTTAGCCCAATT GTCACTGTGGAACCAAGAAGACGGAAATTCCATAAACCAATCACAATGACCATTCCGGTGCCCCCGCCC TCAGGAGAAGGTGTATCCAATGGATACAAAGGGGACACTACACCCAATCTGCGTCTTCTCTGTAGCATT ACAGGGGGCACTTCGCCTGCTCAGTGGGAAGACATCACAGGAACAACTCCTTTGACGTTTATAAAAGAT TGTGTCTCCTTTACAACCAATGTTTCAGCCAGATTTTGGCTTGCAGACTGCCATCAAGTTTTAGAAACT GTGGGGTTAGCCACGCAACTGTACAGAGAATTGATATGTGTTCCATATATGGCCAAGTTTGTTGTTTTT GCCAAAATGAATGATCCCGTAGAATCTTCCTTGCGATGTTTCTGCATGACAGATGACAAAGTGGACAAA ACTTTAGAGCAACAAGAGAATTTTGAGGAAGTCGCAAGAAGCAAAGATATTGAGGTTCTGGAAGGAAAA CCTATTTATGTTGATTGTTATGGAAATTTGGCCCCACTTACCAAAGGAGGACAGCAACTTGTTTTTAAC TTTTATTCTTTCAAAGAAAATAGACTGCCATTTTCCATCAAGATTAGAGACACCAGCCAAGAGCCCTGT GGTCGTCTGTCTTTTCTGAAAGAACCAAAGACAACAAAAGGACTGCCTCAAACAGCGGTTTGCAACTTA AATATCACTCTGCCAGCACATAAAAAGATTGAGAAAACAGATAGACGACAGAGCTTCGCATCCTTAGCT TTACGTAAGCGCTACAGCTACTTGACTGAGCCTGGAATGAGTCCACAGAGTCCATGTGAACGGACAGAT ATCAGGATGGCAATAGTAGCCGATCACCTGGGACTTAGTTGGACAGAACTGGCAAGGGAACTGAATTTT TCAGTGGATGAAATCAATCAAATACGTGTGGAAAATCCAAATTCTTTAATTTCTCAGAGCTTCATGTTA TTAAAAAAATGGGTTACCAGAGACGGAAAAAATGCCACAACTGATGCCTTAACTTCGGTCTTGACAAAA ATTAATCGAATAGATATAGTGACACTGCTAGAAGGACCAATATTTGATTATGGAAATATTTCAGGCACC AGAAGTTTTGCAGATGAGAACAATGTTTTCCATGACCCTGTTGATGGTTATCCTTCCCTTCAAGTGGAA CTGGAAACCCCCACAGGGTTGCACTACACACCACCTACCCCTTTCCAGCAAGATGATTATTTTAGTGAT ATCTCTAGCATAGAATCTCCCCTTAGAACCCCTAGTAGACTGAGTGATGGGCTAGTGCCTTCCCAGGGG AACATAGAGCATTCCGCAGATGGACCTCCAGTCGTAACTGCAGAAGACGCTTCCTTAGAAGACAGCAAA CTGGAAGACTCAGTGCCTTTAACAGAAATGCCTGAAGCAGTGGATGTAGATGAGAGCCAGTTGGAGAAT GTATGTCTGAGTTGGCAGAATGAGACATCAAGTGGAAACCTAGAGTCCTGCGCTCAAGCTCGAAGAGTA ACTGGTGGGTTACTAGATCGACTGGATGACAGCCCTGACCAGTGTAGAGATTCCATTACCTCATATCTC AAAGGAGAAGCTGGCAAATTTGAAGCAAATGGAAGCCATACAGAAATCACTCCAGAAGCAAAGACAAAA TCTTACTTTCCAGAATCCCAAAATGATGTAGGAAAACAGAGTACCAAGGAAACTCTGAAACCAAAAATA CATGGATCTGGTCATGTTGAAGAACCAGCATCACCACTAGCAGCATATCAGAAATCTCTAGAAGAAACC AGCAAGCTTATAATAGAAGAGACTAAACCCTGTGTGCCTGTCAGTATGAAAAAGATGAGTAGGACTTCT CCAGCAGATGGCAAGCCAAGGCTTAGCCTCCATGAAGAAGAGGGGTCCAGTGGGTCTGAGCAAAAGCAG GGAGAAGGTTTTAAGGTGAAAACGAAGAAAGAAATCCGGCATGTGGAAAAGAAGAGCCACTCGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001204403
Insert Size	5586 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204403.1
RefSeq Size	9356 bp
RefSeq ORF	5586 bp
Locus ID	288
UniProt ID	Q12955
Protein Families	Druggable Genome
MW	202.6 kDa
Gene Summary	Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 3 is an immunologically distinct gene product from ankyrins 1 and 2, and was originally found at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, initiates translation at an alternate start codon, and lacks an alternate in-frame exon compared to variant 1. The encoded protein (isoform 3) has a distinct N-terminus and is considerably shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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