Ankyrin G (ANK3) (NM_001204403) Human Tagged ORF Clone

CAT#: RC235342

  • TrueORF®

ANK3 (Myc-DDK tagged) - Homo sapiens ankyrin 3, node of Ranvier (ankyrin G) (ANK3), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_001204403" in other vectors (2)

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CNY 20,016.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


ANK3 / ANKYRIN-G Goat Polyclonal Antibody
    • 50 ug

CNY 5,324.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ANKYRIN-G; MRT37
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC235342 representing NM_001204403
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCCTCCGCCTCCTCCTCTCCCGCGGGCACCGAGGACTCCGCTCCTGCCCAGGGTGGCTTTGGAT
CAGATTATTCACGAAGCTCTAGAAAGTCTGATGCCAATGCAAGTTACTTAAGAGCAGCTCGAGCTGGACA
CCTTGAAAAGGCCCTCGACTACATAAAAAATGGAGTTGACATCAACATTTGCAATCAGAATGGGTTGAAC
GCTCTCCACCTTGCTTCCAAAGAAGGCCATGTAGAGGTTGTTTCTGAGCTGCTGCAGAGAGAAGCCAATG
TGGATGCAGCTACAAAGAAAGGAAACACAGCATTGCACATCGCATCTTTGGCTGGGCAAGCAGAGGTGGT
AAAAGTCTTGGTTACAAATGGAGCCAATGTCAATGCACAATCTCAGAATGGTTTCACGCCATTGTATATG
GCAGCCCAGGAAAATCACCTGGAAGTTGTCAAGTTTCTTCTTGACAATGGTGCAAGCCAGAGCCTAGCCA
CAGAGGATGGCTTCACACCATTGGCAGTGGCTTTGCAACAAGGTCACGACCAAGTCGTTTCGCTCCTGCT
AGAGAATGACACCAAAGGAAAAGTGCGTCTCCCAGCTCTTCATATCGCGGCCCGAAAAGACGACACGAAA
GCCGCCGCCCTGCTGCTGCAGAATGACAACAATGCAGATGTGGAATCAAAGAGTGGCTTCACTCCGCTCC
ACATAGCTGCTCACTATGGAAATATCAATGTAGCCACGTTGCTGTTAAACCGAGCGGCTGCTGTGGATTT
CACCGCAAGGAATGACATCACTCCTTTACATGTTGCATCAAAAAGAGGAAATGCAAATATGGTAAAACTA
TTGCTCGATCGAGGAGCTAAAATCGATGCCAAAACCAGGGATGGTCTGACACCACTGCACTGTGGAGCAA
GGAGTGGCCACGAGCAGGTGGTAGAAATGTTGCTTGATCGAGCTGCCCCCATTCTTTCAAAAACCAAGAA
TGGATTATCTCCATTGCACATGGCCACACAAGGGGATCATTTAAACTGCGTCCAGCTTCTCCTCCAGCAT
AATGTACCCGTGGATGATGTCACCAATGACTACCTGACTGCCCTACACGTGGCTGCCCACTGTGGCCATT
ACAAAGTTGCCAAGGTTCTCTTGGATAAGAAAGCTAACCCCAATGCCAAAGCCCTGAATGGCTTTACCCC
TCTTCATATTGCCTGCAAGAAGAATCGAATTAAAGTAATGGAACTCCTTCTGAAACACGGTGCATCCATC
CAAGCTGTAACCGAGTCGGGCCTTACCCCAATCCATGTTGCTGCCTTCATGGGGCATGTAAATATTGTAT
CACAACTAATGCATCATGGAGCCTCACCAAACACCACCAATGTGAGAGGAGAAACAGCACTGCACATGGC
AGCTCGCTCCGGCCAAGCTGAAGTTGTGCGGTATCTGGTACAAGACGGAGCTCAGGTAGAAGCTAAAGCT
AAGGATGACCAAACACCACTCCACATTTCAGCCCGACTGGGGAAAGCAGACATAGTACAACAGCTGTTGC
AGCAAGGGGCATCTCCAAATGCAGCCACAACTTCTGGGTACACCCCACTTCACCTTTCCGCCCGAGAGGG
GCATGAGGATGTGGCCGCGTTCCTTTTGGATCATGGAGCGTCTTTATCTATAACAACAAAGAAAGGATTT
ACTCCTCTTCATGTGGCAGCAAAATATGGAAAGCTTGAAGTCGCCAATCTCCTGCTACAGAAAAGTGCAT
CTCCAGATGCTGCTGGGAAGAGCGGGCTAACACCACTGCATGTAGCTGCACATTACGATAATCAGAAAGT
GGCCCTTCTGCTTTTGGACCAAGGAGCCTCACCTCACGCAGCCGCAAAGAATGGTTATACGCCACTGCAC
ATCGCTGCCAAAAAGAACCAGATGGACATAGCGACAACTCTGCTGGAATATGGTGCTGATGCCAACGCAG
TTACCCGGCAAGGAATTGCTTCCGTCCATCTCGCAGCTCAGGAAGGGCACGTGGACATGGTGTCGCTGCT
CCTCGGTAGAAATGCGAATGTGAACCTGAGCAATAAGAGCGGCCTGACCCCACTCCATTTGGCTGCTCAA
GAAGATCGAGTGAATGTGGCAGAAGTCCTCGTAAACCAAGGGGCTCATGTGGACGCCCAGACAAAGATGG
GATACACACCACTGCATGTGGGCTGCCACTATGGAAATATCAAGATTGTTAATTTCCTGCTCCAGCATTC
TGCAAAAGTTAATGCCAAAACAAAGAATGGGTATACGCCATTACATCAAGCAGCACAGCAGGGGCATACG
CATATAATAAATGTCTTACTTCAGAACAACGCCTCCCCCAATGAACTCACTGTGAATGGGAATACTGCCC
TTGGCATTGCCCGGCGCCTCGGCTACATCTCAGTAGTGGACACCCTGAAGATAGTGACCGAAGAGACCAT
GACCACAACTACTGTCACAGAGAAGCACAAAATGAATGTTCCAGAAACGATGAATGAAGTTCTTGATATG
TCTGATGATGAAGTTCGTAAAGCCAATGCCCCTGAAATGCTCAGTGATGGCGAATATATCTCAGATGTTG
AAGAAGGTGAAGATGCAATGACCGGGGACACAGACAAATATCTTGGGCCACAGGACCTTAAGGAATTGGG
TGATGATTCCCTGCCTGCAGAGGGTTACATGGGCTTTAGTCTCGGAGCGCGTTCTGCCAGCCTCCGCTCC
TTCAGTTCGGATAGGTCTTACACCTTGAACAGAAGCTCCTATGCACGGGACAGCATGATGATTGAAGAAC
TCCTTGTGCCATCCAAAGAGCAGCATCTAACATTCACAAGGGAATTTGATTCAGATTCTCTTAGACATTA
CAGCTGGGCTGCAGACACCTTAGACAATGTCAATCTTGTTTCAAGCCCCATTCATTCTGGGTTTCTGGTT
AGCTTTATGGTGGACGCGAGAGGGGGCTCCATGAGAGGAAGCCGTCATCACGGGATGAGAATCATCATTC
CTCCACGCAAGTGTACGGCCCCCACTCGAATCACCTGCCGTTTGGTAAAGAGACATAAACTGGCCAACCC
ACCCCCCATGGTGGAAGGAGAGGGATTAGCCAGTAGGCTGGTAGAAATGGGTCCTGCAGGGGCACAATTT
TTAGGCCCTGTCATAGTGGAAATCCCTCACTTTGGGTCCATGAGAGGAAAAGAGAGAGAACTCATTGTTC
TTCGAAGTGAAAATGGTGAAACTTGGAAGGAGCATCAGTTTGACAGCAAAAATGAAGATTTAACCGAGTT
ACTTAATGGCATGGATGAAGAACTTGATAGCCCAGAAGAGTTAGGGAAAAAGCGTATCTGCAGGATTATC
ACGAAAGATTTCCCCCAGTATTTTGCAGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAGATTGGTCCTG
AAGGTGGAATTCTGAGCAGCACCACAGTGCCCCTTGTTCAAGCATCTTTCCCAGAGGGTGCCCTAACTAA
AAGAATTCGAGTGGGCCTCCAGGCCCAGCCTGTTCCAGATGAAATTGTGAAAAAGATCCTTGGAAACAAA
GCAACTTTTAGCCCAATTGTCACTGTGGAACCAAGAAGACGGAAATTCCATAAACCAATCACAATGACCA
TTCCGGTGCCCCCGCCCTCAGGAGAAGGTGTATCCAATGGATACAAAGGGGACACTACACCCAATCTGCG
TCTTCTCTGTAGCATTACAGGGGGCACTTCGCCTGCTCAGTGGGAAGACATCACAGGAACAACTCCTTTG
ACGTTTATAAAAGATTGTGTCTCCTTTACAACCAATGTTTCAGCCAGATTTTGGCTTGCAGACTGCCATC
AAGTTTTAGAAACTGTGGGGTTAGCCACGCAACTGTACAGAGAATTGATATGTGTTCCATATATGGCCAA
GTTTGTTGTTTTTGCCAAAATGAATGATCCCGTAGAATCTTCCTTGCGATGTTTCTGCATGACAGATGAC
AAAGTGGACAAAACTTTAGAGCAACAAGAGAATTTTGAGGAAGTCGCAAGAAGCAAAGATATTGAGGTTC
TGGAAGGAAAACCTATTTATGTTGATTGTTATGGAAATTTGGCCCCACTTACCAAAGGAGGACAGCAACT
TGTTTTTAACTTTTATTCTTTCAAAGAAAATAGACTGCCATTTTCCATCAAGATTAGAGACACCAGCCAA
GAGCCCTGTGGTCGTCTGTCTTTTCTGAAAGAACCAAAGACAACAAAAGGACTGCCTCAAACAGCGGTTT
GCAACTTAAATATCACTCTGCCAGCACATAAAAAGATTGAGAAAACAGATAGACGACAGAGCTTCGCATC
CTTAGCTTTACGTAAGCGCTACAGCTACTTGACTGAGCCTGGAATGAGTCCACAGAGTCCATGTGAACGG
ACAGATATCAGGATGGCAATAGTAGCCGATCACCTGGGACTTAGTTGGACAGAACTGGCAAGGGAACTGA
ATTTTTCAGTGGATGAAATCAATCAAATACGTGTGGAAAATCCAAATTCTTTAATTTCTCAGAGCTTCAT
GTTATTAAAAAAATGGGTTACCAGAGACGGAAAAAATGCCACAACTGATGCCTTAACTTCGGTCTTGACA
AAAATTAATCGAATAGATATAGTGACACTGCTAGAAGGACCAATATTTGATTATGGAAATATTTCAGGCA
CCAGAAGTTTTGCAGATGAGAACAATGTTTTCCATGACCCTGTTGATGGTTATCCTTCCCTTCAAGTGGA
ACTGGAAACCCCCACAGGGTTGCACTACACACCACCTACCCCTTTCCAGCAAGATGATTATTTTAGTGAT
ATCTCTAGCATAGAATCTCCCCTTAGAACCCCTAGTAGACTGAGTGATGGGCTAGTGCCTTCCCAGGGGA
ACATAGAGCATTCCGCAGATGGACCTCCAGTCGTAACTGCAGAAGACGCTTCCTTAGAAGACAGCAAACT
GGAAGACTCAGTGCCTTTAACAGAAATGCCTGAAGCAGTGGATGTAGATGAGAGCCAGTTGGAGAATGTA
TGTCTGAGTTGGCAGAATGAGACATCAAGTGGAAACCTAGAGTCCTGCGCTCAAGCTCGAAGAGTAACTG
GTGGGTTACTAGATCGACTGGATGACAGCCCTGACCAGTGTAGAGATTCCATTACCTCATATCTCAAAGG
AGAAGCTGGCAAATTTGAAGCAAATGGAAGCCATACAGAAATCACTCCAGAAGCAAAGACAAAATCTTAC
TTTCCAGAATCCCAAAATGATGTAGGAAAACAGAGTACCAAGGAAACTCTGAAACCAAAAATACATGGAT
CTGGTCATGTTGAAGAACCAGCATCACCACTAGCAGCATATCAGAAATCTCTAGAAGAAACCAGCAAGCT
TATAATAGAAGAGACTAAACCCTGTGTGCCTGTCAGTATGAAAAAGATGAGTAGGACTTCTCCAGCAGAT
GGCAAGCCAAGGCTTAGCCTCCATGAAGAAGAGGGGTCCAGTGGGTCTGAGCAAAAGCAGGGAGAAGGTT
TTAAGGTGAAAACGAAGAAAGAAATCCGGCATGTGGAAAAGAAGAGCCACTCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC235342 representing NM_001204403
Red=Cloning site Green=Tags(s)

MASSASSSPAGTEDSAPAQGGFGSDYSRSSRKSDANASYLRAARAGHLEKALDYIKNGVDINICNQNGLN
ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYM
AAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTK
AAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKL
LLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQH
NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASI
QAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKA
KDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGF
TPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLH
IAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQ
EDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHT
HIINVLLQNNASPNELTVNGNTALGIARRLGYISVVDTLKIVTEETMTTTTVTEKHKMNVPETMNEVLDM
SDDEVRKANAPEMLSDGEYISDVEEGEDAMTGDTDKYLGPQDLKELGDDSLPAEGYMGFSLGARSASLRS
FSSDRSYTLNRSSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVSSPIHSGFLV
SFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRLVEMGPAGAQF
LGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDLTELLNGMDEELDSPEELGKKRICRII
TKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASFPEGALTKRIRVGLQAQPVPDEIVKKILGNK
ATFSPIVTVEPRRRKFHKPITMTIPVPPPSGEGVSNGYKGDTTPNLRLLCSITGGTSPAQWEDITGTTPL
TFIKDCVSFTTNVSARFWLADCHQVLETVGLATQLYRELICVPYMAKFVVFAKMNDPVESSLRCFCMTDD
KVDKTLEQQENFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQ
EPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKKIEKTDRRQSFASLALRKRYSYLTEPGMSPQSPCER
TDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFMLLKKWVTRDGKNATTDALTSVLT
KINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDGYPSLQVELETPTGLHYTPPTPFQQDDYFSD
ISSIESPLRTPSRLSDGLVPSQGNIEHSADGPPVVTAEDASLEDSKLEDSVPLTEMPEAVDVDESQLENV
CLSWQNETSSGNLESCAQARRVTGGLLDRLDDSPDQCRDSITSYLKGEAGKFEANGSHTEITPEAKTKSY
FPESQNDVGKQSTKETLKPKIHGSGHVEEPASPLAAYQKSLEETSKLIIEETKPCVPVSMKKMSRTSPAD
GKPRLSLHEEEGSSGSEQKQGEGFKVKTKKEIRHVEKKSHS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001204403
ORF Size 5583 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001204403.2
RefSeq Size 9356 bp
RefSeq ORF 5586 bp
Locus ID 288
UniProt ID Q12955
Protein Families Druggable Genome
MW 203 kDa
Gene Summary Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 3 is an immunologically distinct gene product from ankyrins 1 and 2, and was originally found at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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