GNPDA2 (NM_001270880) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GNP2; SB52
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330881 representing NM_001270880. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGGCTTGTAATTCTTGATAACTATGACTTGGCTAGTGAATGGGCAGCCAAATACATCTGTAATCGC ATCATTCAGTTCAAACCTGGACAGGACAGATATTTTACACTGGGTTTACCAACAGGACTTCCAAGAAAT CATCCTGAAAGCTACCATTCTTATATGTGGAATAATTTTTTTAAGCATATCGATATAGATCCTAATAAT GCACATATCCTTGACGGGAATGCTGCAGATTTACAAGCAGAATGTGATGCTTTTGAAAACAAAATAAAA GAAGCTGGAGGAATAGATCTTTTTGTTGGAGGAATTGGTCCAGATGGTCATATCGCTTTCAATGAGCCT GGATCCAGTTTAGTGTCAAGGACAAGATTAAAGACTCTAGCAATGGATACCATCTTGGCAAATGCCAAA TATTTTGATGGAGATTTATCAAAAGTGCCAACTATGGCTCTAACTGTTGGTGTGGGGACAGTGATGGAT GCTAGAGAAGTAATGATCCTTATAACAGGGGCACACAAGGCATTTGCCCTGTACAAAGCAATAGAAGAA GGAGTCAATCACATGTGGACTGTTTCCGCTTTCCAGCAGCATCCCCGGACTATTTTTGTATGCGATGAA GATGCTACTTTAGAATTAAGAGTTAAAACTGTGAAATACTTTAAAGGTCTAATGCATGTGCACAATAAA CTTGTGGATCCACTATTCAGTATGAAAGATGGAAACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001270880
Insert Size	729 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270880.1
RefSeq Size	2211 bp
RefSeq ORF	729 bp
Locus ID	132789
UniProt ID	Q8TDQ7
Protein Pathways	Amino sugar and nucleotide sugar metabolism, Metabolic pathways
MW	27.1 kDa
Gene Summary	The protein encoded by this gene is an allosteric enzyme that catalyzes the reversible reaction converting D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. Variations of this gene have been reported to be associated with influencing body mass index and susceptibility to obesity. A pseudogene of this gene is located on chromosome 9. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1.

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