PDE8A (NM_001243137) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HsT19550
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330089 representing NM_001243137. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGATTTCATCAAGATCAACTTCAGGTACTTTTAGTGTTTACCAAAGAAGATAACCAATGTAATGGA TTCTGCAGGGCATGTGAAAAAGCAGGGTTTAAGTGTACAGTTACCAAGGAGGCTCAGGCTGTCCTTGCC TGTTTCCTGGACAAACATCATGACATTATCATCATAGACCACAGAAATCCTCGACAGCTGGATGCAGAG GCACTGTGCAGGTCTATCAGATCATCAAAACTCTCAGAAAACACAGTTATTGTTGGTGTAGTACGCAGG GTGGATAGAGAAGAGTTGTCCGTAATGCCTTTCATTTCTGCTGGATTTACAAGGAGGTATGTAGAAAAC CCCAACATCATGGCCTGCTACAATGAACTGCTCCAGCTGGAGTTTGGAGAGGTGCGATCACAACTGAAA CTCAGGGCTTGTAACTCAGTATTCACTGCATTAGAAAACAGTGAAGATGCAATTGAAATTACAAGCGAA GACCGTTTTATACAGTATGCAAATCCTGCATTTGAAACAACAATGGGCTATCAGTCAGGTGAATTAATA GGGAAGGAGTTAGGAGAAGTGCCTATAAATGAAAAAAAGGCTGACTTGCTCGATACTATAAATTCATGC ATCAGGATAGGCAAGGAGTGGCAAGGAATTTACTATGCCAAAAAGAAAAACGGAGATAATATACAACAA AATGTGAAGATAATACCTGTCATTGGACAGGGAGGAAAAATTAGACACTATGTGTCCATTATCAGAGTG TGCAATGGCAACAATAAGGCTGAGAAAATATCCGAATGTGTTCAGTCTGACACTCATACAGATAATCAG ACAGGCAAACATAAAGACAGGAGAAAAGGCTCACTAGACGTCAAAGCTGTTGCCTCCCGTGCAACTGAA GTTTCCAGCCAGAGACGACACTCTTCCATGGCCCGGATACATTCCATGACAATTGAGGCGCCCATCACC AAGGTAATCAATATTATCAATGCTGCCCAGGAAAGTAGTCCCATGCCTGTGACAGAAGCCCTAGACCGT GTGCTGGAAATTCTAAGAACCACTGAGTTATATTCACCACAGTTTGGTGCTAAAGATGATGATCCCCAT GCCAATGACCTTGTTGGGGGCTTAATGTCTGATGGTTTGCGAAGACTATCAGGGAATGAATATGTTCTT TCAACAAAAAACACTCAAATGGTTTCAAGCAATATAATCACTCCCATCTCCCTTGATGATGTCCCACCA CGGATAGCTCGGGCCATGGAAAATGAGGAATACTGGGACTTTGATATTTTTGAACTGGAGGCTGCCACC CACAATAGGCCTTTGATTTATCTTGGTCTCAAAATGTTTGCTCGCTTTGGAATCTGTGAATTCTTACAC TGCTCCGAGTCAACGCTAAGATCATGGTTACAAATTATCGAAGCCAATTATCATTCCTCCAATCCCTAC CACAATTCTACACATTCTGCTGATGTGCTTCATGCCACTGCCTATTTTCTCTCCAAGGAGAGGATAAAG GAAACTTTAGATCCAATTGATGAGGTCGCTGCACTCATCGCAGCCACCATTCATGATGTGGATCACCCT GGGAGAACCAACTCCTTCCTGTGTAATGCTGGAAGTGAGCTGGCCATTTTGTACAATGACACTGCTGTG CTGGAGAGCCACCATGCGGCCTTGGCCTTCCAGCTGACCACTGGAGATGATAAATGCAATATATTTAAA AACATGGAGAGGAATGATTATCGGACACTGCGCCAGGGGATTATCGACATGGTCTTAGCCACAGAAATG ACAAAGCACTTTGAGCATGTCAACAAATTTGTCAACAGCATCAACAAACCCTTGGCAACACTAGAAGAA AATGGGGAAACTGATAAAAACCAGGAAGTGATAAACACTATGCTTAGGACTCCAGAGAACCGGACCCTA ATCAAACGAATGCTGATTAAATGTGCTGATGTGTCCAATCCCTGCCGACCCCTGCAGTACTGCATCGAG TGGGCTGCACGCATTTCGGAAGAATATTTTTCTCAGACTGATGAAGAGAAGCAGCAGGGCTTACCTGTG GTGATGCCAGTGTTTGACAGAAATACCTGCAGCATCCCCAAATCCCAAATCTCTTTCATTGATTACTTC ATCACAGACATGTTTGATGCTTGGGATGCCTTTGTAGACCTGCCTGATTTAATGCAGCATCTTGACAAC AACTTTAAATACTGGAAAGGACTGGACGAAATGAAGCTGCGGAACCTCCGACCACCTCCTGAATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001243137
Insert Size	2274 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243137.1
RefSeq Size	3728 bp
RefSeq ORF	2274 bp
Locus ID	5151
UniProt ID	O60658
Protein Families	Druggable Genome
Protein Pathways	Progesterone-mediated oocyte maturation, Purine metabolism
MW	86 kDa
Gene Summary	The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] Transcript Variant: This variant (3) contains an alternate 5' terminal exon compared to variant 1. This results in translation initiation from an in-frame downstream AUG, and an isoform (3) with a shorter N-terminus compared to isoform 1.

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