PDE8A (NM_001243137) Human Tagged ORF Clone
CAT#: RC233126
- TrueORF®
PDE8A (Myc-DDK tagged) - Homo sapiens phosphodiesterase 8A (PDE8A), transcript variant 3
ORF Plasmid: tGFP
"NM_001243137" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 7,510.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HsT19550 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC233126 representing NM_001243137
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGATTTCATCAAGATCAACTTCAGGTACTTTTAGTGTTTACCAAAGAAGATAACCAATGTAATGGAT TCTGCAGGGCATGTGAAAAAGCAGGGTTTAAGTGTACAGTTACCAAGGAGGCTCAGGCTGTCCTTGCCTG TTTCCTGGACAAACATCATGACATTATCATCATAGACCACAGAAATCCTCGACAGCTGGATGCAGAGGCA CTGTGCAGGTCTATCAGATCATCAAAACTCTCAGAAAACACAGTTATTGTTGGTGTAGTACGCAGGGTGG ATAGAGAAGAGTTGTCCGTAATGCCTTTCATTTCTGCTGGATTTACAAGGAGGTATGTAGAAAACCCCAA CATCATGGCCTGCTACAATGAACTGCTCCAGCTGGAGTTTGGAGAGGTGCGATCACAACTGAAACTCAGG GCTTGTAACTCAGTATTCACTGCATTAGAAAACAGTGAAGATGCAATTGAAATTACAAGCGAAGACCGTT TTATACAGTATGCAAATCCTGCATTTGAAACAACAATGGGCTATCAGTCAGGTGAATTAATAGGGAAGGA GTTAGGAGAAGTGCCTATAAATGAAAAAAAGGCTGACTTGCTCGATACTATAAATTCATGCATCAGGATA GGCAAGGAGTGGCAAGGAATTTACTATGCCAAAAAGAAAAACGGAGATAATATACAACAAAATGTGAAGA TAATACCTGTCATTGGACAGGGAGGAAAAATTAGACACTATGTGTCCATTATCAGAGTGTGCAATGGCAA CAATAAGGCTGAGAAAATATCCGAATGTGTTCAGTCTGACACTCATACAGATAATCAGACAGGCAAACAT AAAGACAGGAGAAAAGGCTCACTAGACGTCAAAGCTGTTGCCTCCCGTGCAACTGAAGTTTCCAGCCAGA GACGACACTCTTCCATGGCCCGGATACATTCCATGACAATTGAGGCGCCCATCACCAAGGTAATCAATAT TATCAATGCTGCCCAGGAAAGTAGTCCCATGCCTGTGACAGAAGCCCTAGACCGTGTGCTGGAAATTCTA AGAACCACTGAGTTATATTCACCACAGTTTGGTGCTAAAGATGATGATCCCCATGCCAATGACCTTGTTG GGGGCTTAATGTCTGATGGTTTGCGAAGACTATCAGGGAATGAATATGTTCTTTCAACAAAAAACACTCA AATGGTTTCAAGCAATATAATCACTCCCATCTCCCTTGATGATGTCCCACCACGGATAGCTCGGGCCATG GAAAATGAGGAATACTGGGACTTTGATATTTTTGAACTGGAGGCTGCCACCCACAATAGGCCTTTGATTT ATCTTGGTCTCAAAATGTTTGCTCGCTTTGGAATCTGTGAATTCTTACACTGCTCCGAGTCAACGCTAAG ATCATGGTTACAAATTATCGAAGCCAATTATCATTCCTCCAATCCCTACCACAATTCTACACATTCTGCT GATGTGCTTCATGCCACTGCCTATTTTCTCTCCAAGGAGAGGATAAAGGAAACTTTAGATCCAATTGATG AGGTCGCTGCACTCATCGCAGCCACCATTCATGATGTGGATCACCCTGGGAGAACCAACTCCTTCCTGTG TAATGCTGGAAGTGAGCTGGCCATTTTGTACAATGACACTGCTGTGCTGGAGAGCCACCATGCGGCCTTG GCCTTCCAGCTGACCACTGGAGATGATAAATGCAATATATTTAAAAACATGGAGAGGAATGATTATCGGA CACTGCGCCAGGGGATTATCGACATGGTCTTAGCCACAGAAATGACAAAGCACTTTGAGCATGTCAACAA ATTTGTCAACAGCATCAACAAACCCTTGGCAACACTAGAAGAAAATGGGGAAACTGATAAAAACCAGGAA GTGATAAACACTATGCTTAGGACTCCAGAGAACCGGACCCTAATCAAACGAATGCTGATTAAATGTGCTG ATGTGTCCAATCCCTGCCGACCCCTGCAGTACTGCATCGAGTGGGCTGCACGCATTTCGGAAGAATATTT TTCTCAGACTGATGAAGAGAAGCAGCAGGGCTTACCTGTGGTGATGCCAGTGTTTGACAGAAATACCTGC AGCATCCCCAAATCCCAAATCTCTTTCATTGATTACTTCATCACAGACATGTTTGATGCTTGGGATGCCT TTGTAGACCTGCCTGATTTAATGCAGCATCTTGACAACAACTTTAAATACTGGAAAGGACTGGACGAAAT GAAGCTGCGGAACCTCCGACCACCTCCTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC233126 representing NM_001243137
Red=Cloning site Green=Tags(s) MRFHQDQLQVLLVFTKEDNQCNGFCRACEKAGFKCTVTKEAQAVLACFLDKHHDIIIIDHRNPRQLDAEA LCRSIRSSKLSENTVIVGVVRRVDREELSVMPFISAGFTRRYVENPNIMACYNELLQLEFGEVRSQLKLR ACNSVFTALENSEDAIEITSEDRFIQYANPAFETTMGYQSGELIGKELGEVPINEKKADLLDTINSCIRI GKEWQGIYYAKKKNGDNIQQNVKIIPVIGQGGKIRHYVSIIRVCNGNNKAEKISECVQSDTHTDNQTGKH KDRRKGSLDVKAVASRATEVSSQRRHSSMARIHSMTIEAPITKVINIINAAQESSPMPVTEALDRVLEIL RTTELYSPQFGAKDDDPHANDLVGGLMSDGLRRLSGNEYVLSTKNTQMVSSNIITPISLDDVPPRIARAM ENEEYWDFDIFELEAATHNRPLIYLGLKMFARFGICEFLHCSESTLRSWLQIIEANYHSSNPYHNSTHSA DVLHATAYFLSKERIKETLDPIDEVAALIAATIHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAAL AFQLTTGDDKCNIFKNMERNDYRTLRQGIIDMVLATEMTKHFEHVNKFVNSINKPLATLEENGETDKNQE VINTMLRTPENRTLIKRMLIKCADVSNPCRPLQYCIEWAARISEEYFSQTDEEKQQGLPVVMPVFDRNTC SIPKSQISFIDYFITDMFDAWDAFVDLPDLMQHLDNNFKYWKGLDEMKLRNLRPPPE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001243137 |
ORF Size | 2271 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001243137.2 |
RefSeq Size | 3728 bp |
RefSeq ORF | 2274 bp |
Locus ID | 5151 |
UniProt ID | O60658 |
Protein Families | Druggable Genome |
Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
MW | 86.5 kDa |
Gene Summary | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] |
Documents
Product Manuals |
FAQs |
SDS |