HCP1 (SLC46A1) (NM_001242366) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	G21; HCP1; PCFT
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329938 representing NM_001242366. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGGGGAGCGCGAGCCCCCCGGAAAAGCCCCGCGCCCGCCCTGCGGCTGCCGTGCTGTGCCGGGGC CCGGTAGAGCCGCTGGTCTTCCTGGCCAACTTTGCCTTGGTCCTGCAGGGCCCGCTCACCACGCAGTAT CTGTGGCACCGCTTCAGCGCCGACCTCGGCTACAATGGCACCCGCCAAAGGGGGGGCTGCAGCAACCGC AGCGCGGACCCCACCATGCAGGAAGTGGAGACCCTTACCTCCCACTGGACCCTCTACATGAACGTGGGC GGCTTCCTGGTGGGGCTCTTCTCGTCCACCCTGCTGGGAGCTTGGAGCGACAGTGTGGGCCGCCGCCCG CTGCTAGTGCTGGCCTCGCTGGGCCTGCTGCTCCAGGCCCTAGTGTCCGTTTTTGTGGTGCAGCTGCAG CTCCACGTCGGCTACTTCGTGCTGGGTCGCATCCTTTGTGCCCTCCTCGGCGACTTCGGTGGCCTTCTG GCTGCTAGCTTTGCGTCCGTGGCAGATGTCAGCTCCAGTCGCAGCCGCACCTTCCGGATGGCCCTGCTG GAAGCCAGCATCGGGGTGGCTGGGATGCTGGCAAGCCTCCTCGGGGGCCACTGGCTCCGGGCCCAGGGT TATGCCAACCCCTTCTGGCTGGCCTTGGCCTTGCTGATAGCCATGACTCTCTATGCAGCTTTCTGCTTT GGTGAGACCTTAAAGGAGCCAAAGTCCACCCGGCTCTTCACGTTCCGTCACCACCGATCCATTGTCCAG CTCTATGTGGCTCCCGCCCCAGAGAAGTCCAGGAAACATTTAGCCCTCTACTCACTGGCCATCTTCGTG GTGATCACTGTGCACTTTGGGGCCCAGGACATCTTAACCCTTTATGAACTAAGCACACCCCTCTGCTGG GACTCCAAACTAATCGGCTATGGTTCTGCAGCTCAGCATCTCCCCTACCTCACCAGCCTGCTGGCCCTG AAGCTCCTGCAGTACTGCCTGGCCGATGCCTGGGTAGCTGAGATCGGCCTGGCCTTCAACATCCTGGGG ATGGTGGTCTTTGCCTTTGCCACTATCACGCCTCTCATGTTCACAGGTGCTCTCTTTTCTGCTGTGGCC TGTGTGAATAGCCTGGCCATGCTGACGGCCTCCGGCATCTTCAACTCACTCTACCCAGCCACTCTGAAC TTTATGAAGGGGTTCCCCTTCCTCCTGGGAGCTGGCCTCCTGCTCATCCCGGCTGTTCTGATTGGGATG CTGGAAAAGGCTGATCCTCACCTCGAGTTCCAGCAGTTTCCCCAGAGCCCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001242366
Insert Size	1296 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242366.2
RefSeq Size	6426 bp
RefSeq ORF	1296 bp
Locus ID	113235
UniProt ID	Q96NT5
Protein Families	Transmembrane
MW	46.6 kDa
Gene Summary	This gene encodes a transmembrane proton-coupled folate transporter protein that facilitates the movement of folate and antifolate substrates across cell membranes, optimally in acidic pH environments. This protein is also expressed in the brain and choroid plexus where it transports folates into the central nervous system. This protein further functions as a heme transporter in duodenal enterocytes, and potentially in other tissues like liver and kidney. Its localization to the apical membrane or cytoplasm of intestinal cells is modulated by dietary iron levels. Mutations in this gene are associated with autosomal recessive hereditary folate malabsorption disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (2, also known as HCP-1B) lacks an in-frame coding exon compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment (containing one of the transmembrane domains) compared to isoform 1. This isoform was shown to have a different subcellular localization compared to the longer isoform 1 (PMID:17947394).

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