PIGO (NM_001201484) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HPMRS2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC329629 representing NM_001201484. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCAGAAAGCCTCAGTGTTGCTCTTCCTGGCCTGGGTCTGCTTCCTCTTCTACGCTGGCATTGCCCTC TTCACCAGTGGCTTCCTGCTCACCCGTTTGGAGCTCACCAACCATAGCAGCTGCCAAGAGCCCCCAGGC CCTGGGTCCCTGCCATGGGGGAGCCAAGGGAAACCTGGGGCCTGCTGGATGGCTTCCCGATTTTCGCGG GTTGTGTTGGTGCTGATAGATGCTCTGCGATTTGACTTCGCCCAGCCCCAGCATTCACACGTGCCTAGA GAGCCTCCTGTCTCCCTACCCTTCCTGGGCAAACTAAGCTCCTTGCAGAGGATCCTGGAGATTCAGCCC CACCATGCCCGGCTCTACCGATCTCAGGTTGACCCTCCTACCACCACCATGCAGCGCCTCAAGGCCCTC ACCACTGGCTCACTGCCTACCTTTATTGATGCTGGTAGTAACTTCGCCAGCCACGCCATAGTGGAAGAC AATCTCATTAAGCAGCTCACCAGTGCAGGAAGGCGTGTAGTCTTCATGGGAGATGATACCTGGAAAGAC CTTTTCCCTGGTGCTTTCTCCAAAGCTTTCTTCTTCCCATCCTTCAATGTCAGAGACCTAGACACAGTG GACAATGGCATCCTGGAACACCTCTACCCCACCATGGACAGTGGTGAATGGGACGTGCTGATTGCTCAC TTCCTGGGTGTGGACCACTGTGGCCACAAGCATGGCCCTCACCACCCTGAAATGGCCAAGAAACTTAGC CAGATGGACCAGGTGATCCAGGGACTTGTGGAGCGTCTGGAGAATGACACACTGCTGGTAGTGGCTGGG GACCATGGGATGACCACAAATGGAGACCATGGAGGGGACAGTGAGCTGGAGGTCTCAGCTGCTCTCTTT CTGTATAGCCCCACAGCAGTCTTCCCCAGCACCCCACCAGAGGAGCCAGAGGTGATTCCTCAAGTTAGC CTTGTGCCCACGCTGGCCCTGCTGCTGGGCCTGCCCATCCCATTTGGGAATATCGGGGAAGTGATGGCT GAGCTATTCTCAGGGGGTGAGGACTCCCAGCCCCACTCCTCTGCTTTAGCCCAAGCCTCAGCTCTCCAT CTCAATGCTCAGCAGGTGTCCCGATTTCTTCATACCTACTCAGCTGCTACTCAGGACCTTCAAGCTAAG GAGCTTCATCAGCTGCAGAACCTCTTCTCCAAGGCCTCTGCTGACTACCAGTGGCTTCTCCAGAGCCCC AAGGGGGCTGAGGCGACACTGCCGACTGTGATTGCTGAGCTGCAGCAGTTCCTGCGGGGAGCTCGGGCC ATGTGCATCGAGTCTTGGGCTCGTTTCTCTCTGAGCTTCCTTCTCCTACATCTGCTTGCTGCTGGGATA CCCGTCACCACCCCTGGTCCTTTTACTGTGCCATGGCAGGCAGTCTCGGCTTGGGCCCTCATGGCCACA CAGACCTTCTACTCCACAGGCCACCAGCCTGTCTTTCCAGCCATCCATTGGCATGCAGCCTTCGTGGGA TTCCCAGAGGGTCATGGCTCCTGTACTTGGCTGCCTGCTTTGCTAGTGGGAGCCAACACCTTTGCCTCC CACCTCCTCTTTGCAGTAGGTTGCCCACTGCTCCTGCTCTGGCCTTTCCTGTGTGAGAGTCAAGGGCTG CGGAAGAGACAGCAGCCCCCAGGGAATGAAGCTGATGCCAGAGTCAGACCCGAGGAGGAAGAGGAGCCA CTGATGGAGATGCGGCTCCGGGATGCGCCTCAGCACTTCTATGCAGCACTGCTGCAGCTGGGCCTCAAG TACCTCTTTATCCTTGGTATTCAGATTCTGGCCTGTGCCTTGGCAGCCTCCATCCTTCGCAGGCATCTC ATGGTCTGGAAAGTGTTTGCCCCTAAGTTCATATTTGAGGCTGTGGGCTTCATTGTGAGCAGCGTGGGA CTTCTCCTGGGCATAGCTTTGGTGATGAGAGTGGATGGTGCTGTGAGCTCCTGGTTCAGGCAGCTATTT CTGGCCCAGCAGAGGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001201484
Insert Size	2019 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001201484.1
RefSeq Size	2934 bp
RefSeq ORF	2019 bp
Locus ID	84720
UniProt ID	Q8TEQ8
Protein Families	Transmembrane
Protein Pathways	Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
MW	74 kDa
Gene Summary	This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This protein is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI. At least three alternatively spliced transcripts encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011] Transcript Variant: This variant (3) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (2) has the same N- and C-termini but lacks an internal segment compared to isoform 1. Variants 2 and 3 both encode the same isoform (2).

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