Cullin 7 (CUL7) (NM_001168370) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	3M1; CUL-7; dJ20C7.5; KIAA0076
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC329373 representing NM_001168370. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCCGTGGCTTTTGGCTGGCGGAGCCGCTGGCAGGGACCGGGCCCCACCCTGCCCCAGTGGCTGCC GACTCACGCGGGTGCAGCTCTGTTCCGCGGAGGCACGCCCCCTCCCGTCTCTCCGTCTCGACCCCCTCG CGGGGCCCAGGTGCCAGGATGGTGGGAGAACTCCGCTACAGGGAATTCAGGGTGCCCCTGGGGCCCGGC TTACATGCCTATCCTGATGAGCTGATCCGCCAGCGCGTGGGCCATGATGGGCATCCTGAGTACCAGATC CGTTGGCTCATCCTGCGGCGTGGCGATGAGGGGGACGGGGGCTCTGGCCAAGTGGACTGCAAGGCTGAG CACATCCTGCTGTGGATGTCCAAGGATGAGATCTATGCCAACTGCCACAAGATGCTGGGCGAGGATGGC CAGGTCATCGGGCCCTCCCAGGAGTCTGCAGGGGAGGTTGGGGCCCTGGACAAATCTGTGCTGGAGGAG ATGGAAACCGACGTGAAGTCCCTCATTCAGAGAGCCCTTCGGCAGCTGGAGGAGTGTGTGGGCACTATC CCTCCTGCTCCTCTACTTCACACTGTCCACGTGCTCAGCGCCTATGCCAGCATTGAGCCCCTCACTGGA GTATTCAAGGACCCAAGGGTCCTGGACTTGCTCATGCACATGTTGAGTAGTCCTGATTATCAGATTCGC TGGAGTGCAGGCCGGATGATACAAGCCCTGTCCTCCCATGACGCTGGTGAGGGGCAGTGTGGGGAGGAA GGGAAAGCAGGAGAAGGGCTGGGTCGGCTCAGGGACTCACAGGACACTGTGGCAGGAGCCTCTGATCTC ATCAGGACCCGGACTCAGATCCTTCTGTCACTGAGCCAACAAGAAGCCATTGAGAAACACCTGGATTTT GACAGCCGCTGTGCTCTGCTAGCACTGTTTGCACAGGCCACGCTCTCTGAACACCCCATGTCTTTCGAG GGCATTCAGCTACCACAGGTCCCAGGAAGGGTGCTCTTCTCCCTGGTGAAGCGGTATTTGCATGTCACC TCGCTCCTGGATCAGCTGAACGACAGTGCTGCGGAGCCAGGAGCCCAGAACACCTCTGCTCCTGAGGAG TTGAGTGGGGAGAGGGGTCAACTGGAGCTGGAGTTCAGTATGGCCATGGGCACCCTGATCTCGGAGCTG GTGCAAGCCATGCGCTGGGACCAGGCCTCAGACAGACCAAGGAGCTCAGCACGGTCCCCCGGTTCCATC TTCCAGCCTCAGCTGGCAGATGTGAGCCCAGGGCTCCCCGCTGCCCAGGCTCAGCCCTCCTTCAGGAGG TCAAGACGTTTTCGCCCTCGTTCTGAGTTCGCAAGTGGCAATACCTATGCTTTGTATGTGCGGGACACA CTGCAGCCGGGGATGCGAGTGCGGATGCTGGATGATTATGAGGAGATCAGTGCCGGGGATGAGGGCGAG TTTCGGCAGAGCAACAACGGTGTGCCTCCTGTGCAGGTATTTTGGGAGTCAACAGGCCGCACCTATTGG GTGCACTGGCACATGCTGGAGATCTTGGGCTTTGAGGAAGACATTGAGGACATGGTTGAGGCTGATGAG TACCAAGGGGCAGTGGCCAGTAGAGTCCTGGGTAGAGCCCTGCCTGCCTGGCGCTGGAGGCCCATGACA GAACTCTATGCTGTGCCTTATGTGCTGCCTGAGGATGAGGACACTGAGGAGTGTGAACACCTGACCCTG GCTGAGTGGTGGGAACTCCTCTTCTTCATCAAGAAGCTGGATGGACCTGACCATCAGGAGGTTCTCCAG ATCCTCCAGGAGAACCTAGATGGGGAGATTCTGGATGATGAGATCCTAGCTGAACTGGCCGTGCCCATA GAATTGGCCCAGGACTTGCTGCTGACTCTGCCACAGCGACTCAATGACAGTGCCCTCAGGGACCTGATC AACTGCCATGTCTACAAGAAGTATGGGCCTGAAGCCCTAGCAGGGAACCAAGCCTACCCATCCCTTCTA GAAGCCCAAGAAGATGTCCTCCTGCTAGACGCGCAGGCCCAGGCTAAGGACTCAGAAGATGCAGCCAAA GTGGAAGCAAAAGAACCCCCATCTCAGAGTCCCAACACTCCCCTGCAGCGTCTGGTGGAGGGTTATGGT CCAGCTGGGAAAATCCTCCTGGATCTAGAGCAAGCCCTCAGCTCAGAGGGGACCCAGGAGAACAAGGTC AAGCCACTCCTGCTGCAGCTGCAGCGGCAGCCGCAGCCCTTCCTGGCACTGATGCAGAGCCTGGACACT CCGGAGACTAACAGGACCCTGCACCTGACTGTGCTGAGAATCCTGAAGCAGCTGGTGGACTTCCCCGAG GCACTGCTGCTCCCCTGGCACGAGGCCGTGGATGCCTGCATGGCCTGCCTGCGGTCCCCAAACACTGAT CGAGAGGTGCTCCAGGAACTGATTTTCTTCCTGCACCGCCTGACCTCAGTGAGCAGGGACTATGCCGTG GTGCTGAATCAGCTGGGAGCAAGAGACGCTATCTCCAAGGCCCTGGAAAAGCACCTGGGAAAGCTGGAG CTGGCTCAGGAGCTGCGGGACATGGTGTTCAAGTGTGAGAAGCATGCCCACCTCTACCGCAAACTCATC ACCAACATCCTGGGAGGCTGCATCCAGATGGTGCTGGGCCAGATCGAAGACCACAGACGAACCCACCAA CCCATCAACATCCCTTTCTTTGATGTGTTCCTCAGATACCTGTGCCAGGGCTCCAGTGTGGAAGTGAAG GAGGACAAGTGCTGGGAGAAGGTGGAGGTGTCCTCCAACCCGCACCGGGCCAGCAAGCTGACGGACCAC AACCCCAAGACCTATTGGGAGTCCAACGGCAGCGCCGGCTCCCACTACATCACCCTGCACATGCGCCGG GGCATCCTCATCAGGCAACTGACTCTGCTTGTGGCTAGTGAGGACTCGAGTTACATGCCGGCCCGAGTG GTGGTGTGCGGGGGTGATAGCACTAGCTCTCTTCACACGGAACTCAACTCGGTGAATGTGATGCCCTCT GCCAGCCGGGTGATCCTCCTGGAGAACCTGACCCGCTTCTGGCCCATCATCCAGATCCGCATAAAGCGC TGCCAGCAGGGTGGCATTGATACGCGCATTCGGGGGTTAGAGATCCTAGGCCCCAAGCCCACGTTCTGG CCAGTGTTCCGGGAGCAGCTCTGTCGTCACACACGCCTCTTCTACATGGTTCGGGCACAGGCCTGGAGC CAGGACATGGCAGAGGACCGCAGGAGCCTCCTGCACCTGAGTTCTAGACTCAACGGTGCTCTGCGCCAG GAGCAGAATTTTGCTGACCGCTTCCTCCCTGATGACGAGGCTGCCCAAGCTCTGGGCAAGACCTGCTGG GAGGCCCTGGTCAGCCCCGTGGTGCAGAACATCACCTCCCCTGATGAGGATGGCATTAGCCCCCTGGGT TGGCTGCTGGACCAGTACCTGGAGTGTCAGGAAGCTGTCTTCAACCCCCAGAGCCGCGGCCCAGCTTTC TTCTCGCGGGTGCGCCGTCTCACTCACCTGCTGGTGCATGTCGAGCCCTGTGAGGCACCCCCTCCTGTG GTGGCCACTCCTCGGCCCAAAGGCAGAAACAGAAGCCACGACTGGAGCTCCTTGGCTACCCGGGGCCTT CCAAGCAGCATCATGAGAAACCTGACGCGCTGTTGGCGGGCCGTGGTGGAGAAGCAGGTGAACAATTTT CTGACCTCATCCTGGCGGGATGATGACTTTGTGCCACGCTACTGTGAGCACTTTAATATTCTGCAGAAC TCAAGCTCTGAACTGTTTGGGCCTCGGGCAGCCTTCTTGCTGGCGCTGCAAAATGGCTGTGCGGGAGCC TTGCTGAAGCTCCCTTTTCTCAAAGCTGCCCACGTGAGTGAGCAGTTCGCCCGGCACATTGACCAGCAG ATCCAGGGCAGCCGGATCGGTGGAGCCCAGGAAATGGAGAGGCTGGCACAGCTGCAGCAATGCCTGCAA GCTGTCCTGATTTTCTCCGGCTTGGAGATAGCCACCACTTTTGAGCATTATTACCAGCACTACATGGCG GACCGTCTCCTGGGCGTGGTCTCGAGCTGGCTGGAGGGGGCCGTGCTGGAGCAGATCGGTCCCTGCTTC CCCAACCGCCTCCCCCAGCAGATGTTGCAGAGCCTGAGCACCTCTAAGGAGCTGCAGCGCCAGTTCCAC GTCTACCAGCTCCAGCAGCTGGATCAGGAACTCCTGAAGCTGGAGGATACAGAGAAGAAAATACAGGTG GGCCTTGGGGCCAGTGGCAAGGAGCACAAGAGCGAGAAGGAAGAGGAAGCTGGGGCAGCAGCAGTGGTG GATGTGGCGGAGGGAGAGGAGGAAGAGGAGGAGAATGAGGACCTCTACTATGAAGGGGCAATGCCAGAA GTGTCTGTGCTTGTCCTGTCCCGACACTCCTGGCCTGTTGCCTCAATCTGCCACACACTGAACCCCAGA ACCTGCCTGCCCTCCTACCTGAGGGGCACTTTGAACAGATACTCCAACTTCTACAACAAGAGTCAGAGC CACCCTGCCCTTGAGCGAGGCTCACAGAGGCGACTGCAGTGGACGTGGCTGGGCTGGGCTGAGCTGCAG TTTGGGAACCAGACCCTGCATGTGTCCACCGTGCAGATGTGGCTACTGCTGTATCTCAACGACCTGAAG GCGGTCTCTGTGGAGAGTCTGCTGGCGTTCTCAGGGCTCTCCGCAGACATGCTCAATCAGGCGATTGGG CCCCTCACCTCTTCAAGAGGCCCCCTGGACCTTCACGAGCAAAAGGATATACCAGGAGGGGTCCTCAAG ATTCGAGATGGCAGCAAGGAACCCAGGTCGAGATGGGACATTGTGCGGCTCATCCCACCTCAGACGTAC CTGCAAGCTGAGGGTGAAGACGGCCAGAACTTGGAGAAGAGACGGAATCTTCTGAACTGCCTCATCGTC CGAATCCTCAAGGCCCATGGAGATGAGGGGCTGCACATTGACCAGCTTGTCTGTCTGGTGCTGGAGGCT TGGCAGAAGGGCCCGTGTCCTCCCAGGGGTTTGGTCAGCAGCCTTGGTAAGGGGTCTGCATGCAGCAGC ACTGACGTCCTCTCCTGCATCCTACACCTCCTGGGCAAGGGCACGCTGAGACGCCATGACGACCGGCCC CAGGTGCTGTCCTATGCAGTCCCTGTGACTGTCATGGAGCCTCACACTGAGTCCCTGAACCCAGGCTCC TCAGGCCCCAACCCACCCCTCACCTTCCATACCCTACAGATTCGCTCCCGGGGTGTGCCCTATGCCTCC TGCACTGCCACCCAGAGCTTCTCTACCTTCCGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001168370
Insert Size	5349 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001168370.1
RefSeq Size	5527 bp
RefSeq ORF	5349 bp
Locus ID	9820
UniProt ID	Q14999
Protein Pathways	Ubiquitin mediated proteolysis
MW	199.8 kDa
Gene Summary	The protein encoded by this gene is a component of an E3 ubiquitin-protein ligase complex. The encoded protein interacts with TP53, CUL9, and FBXW8 proteins. Defects in this gene are a cause of 3M syndrome type 1 (3M1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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